Details
| Original language | English |
|---|---|
| Article number | 108 |
| Number of pages | 19 |
| Journal | Biosensors |
| Volume | 10 |
| Issue number | 9 |
| Publication status | Published - 26 Aug 2020 |
Abstract
Aptamers, a group of nucleic acids which can specifically bind to a target molecule, have drawn extensive interest over the past few decades. For analytics, aptamers represent a viable alternative to gold-standard antibodies due to their oligonucleic nature combined with advantageous properties, including higher stability in harsh environments and longer shelf-life. Indeed, over the last decade, aptamers have been used in numerous bioanalytical assays and in various point-of-care testing (POCT) platforms. The latter allows for rapid on-site testing and can be performed outside a laboratory by unskilled labor. Aptamer technology for POCT is not limited just to medical diagnostics; it can be used for a range of applications, including environmental monitoring and quality control. In this review, we critically examine the use of aptamers in POCT with an emphasis on their advantages and limitations. We also examine the recent success of aptasensor technology and how these findings pave the way for the analysis of small molecules in POCT and other health-related applications. Finally, the current major limitations of aptamers are discussed, and possible approaches for overcoming these challenges are presented.
Keywords
- Aptamer, Healthcare, POCT, Point-of-care testing, Small molecules
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Clinical Biochemistry
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In: Biosensors, Vol. 10, No. 9, 108, 26.08.2020.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Aptasensors for point-of-care detection of small molecules
AU - Prante, Marc
AU - Segal, Ester
AU - Scheper, Thomas
AU - Bahnemann, Janina
AU - Walter, Johanna
N1 - Funding information: This research received external funding by the DFG with the project number 279/32-2.
PY - 2020/8/26
Y1 - 2020/8/26
N2 - Aptamers, a group of nucleic acids which can specifically bind to a target molecule, have drawn extensive interest over the past few decades. For analytics, aptamers represent a viable alternative to gold-standard antibodies due to their oligonucleic nature combined with advantageous properties, including higher stability in harsh environments and longer shelf-life. Indeed, over the last decade, aptamers have been used in numerous bioanalytical assays and in various point-of-care testing (POCT) platforms. The latter allows for rapid on-site testing and can be performed outside a laboratory by unskilled labor. Aptamer technology for POCT is not limited just to medical diagnostics; it can be used for a range of applications, including environmental monitoring and quality control. In this review, we critically examine the use of aptamers in POCT with an emphasis on their advantages and limitations. We also examine the recent success of aptasensor technology and how these findings pave the way for the analysis of small molecules in POCT and other health-related applications. Finally, the current major limitations of aptamers are discussed, and possible approaches for overcoming these challenges are presented.
AB - Aptamers, a group of nucleic acids which can specifically bind to a target molecule, have drawn extensive interest over the past few decades. For analytics, aptamers represent a viable alternative to gold-standard antibodies due to their oligonucleic nature combined with advantageous properties, including higher stability in harsh environments and longer shelf-life. Indeed, over the last decade, aptamers have been used in numerous bioanalytical assays and in various point-of-care testing (POCT) platforms. The latter allows for rapid on-site testing and can be performed outside a laboratory by unskilled labor. Aptamer technology for POCT is not limited just to medical diagnostics; it can be used for a range of applications, including environmental monitoring and quality control. In this review, we critically examine the use of aptamers in POCT with an emphasis on their advantages and limitations. We also examine the recent success of aptasensor technology and how these findings pave the way for the analysis of small molecules in POCT and other health-related applications. Finally, the current major limitations of aptamers are discussed, and possible approaches for overcoming these challenges are presented.
KW - Aptamer
KW - Healthcare
KW - POCT
KW - Point-of-care testing
KW - Small molecules
UR - http://www.scopus.com/inward/record.url?scp=85090173466&partnerID=8YFLogxK
U2 - 10.3390/bios10090108
DO - 10.3390/bios10090108
M3 - Review article
C2 - 32859075
VL - 10
JO - Biosensors
JF - Biosensors
IS - 9
M1 - 108
ER -