Details
| Original language | English |
|---|---|
| Pages (from-to) | 4991-5003 |
| Number of pages | 13 |
| Journal | The analyst |
| Volume | 145 |
| Issue number | 14 |
| Publication status | Published - 4 Jun 2020 |
Abstract
Over the past decade aptamers have emerged as a promising class of bioreceptors for biosensing applications with significant advantages over conventional antibodies. However, experimental studies comparing aptasensors and immunosensors, under equivalent conditions, are limited and the results are inconclusive, in terms of benefits and limitations of each bioreceptor type. In the present work, the performance of aptamer and antibody bioreceptors for the detection of a his-tagged protein, used as a model target, is compared. The bioreceptors are immobilized onto a nanostructured porous silicon (PSi) thin film, used as the optical transducer, and the target protein is detected in a real-time and label-free format by reflective interferometric Fourier transform spectroscopy. For the antibodies, random-oriented immobilization onto the PSi nanostructure results in a poor biosensing performance. Contrary, Fc-oriented immobilization of the antibodies shows a similar biosensing performance to that exhibited by the aptamer-based biosensor, in terms of binding rate, dynamic detection range, limit of detection and selectivity. The aptasensor outperforms in terms of its reusability and storability, while the immunosensor could not be regenerated for subsequent experiments.
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Environmental Science(all)
- Environmental Chemistry
- Chemistry(all)
- Spectroscopy
- Chemistry(all)
- Electrochemistry
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In: The analyst, Vol. 145, No. 14, 04.06.2020, p. 4991-5003.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Aptamers vs. antibodies as capture probes in optical porous silicon biosensors
AU - Arshavsky-Graham, Sofia
AU - Urmann, Katharina
AU - Salama, Rachel
AU - Massad-Ivanir, Naama
AU - Walter, Johanna Gabriela
AU - Scheper, Thomas
AU - Segal, Ester
N1 - Funding Information: This work was funded by the German Research Foundation under the grant SCHE 279/32-1. We thank Prof. Ayelet Fishman and her group at the Department for Biotechnology and Food Engineering at the Technion Israel Institute of Technology, for supplying target proteins. ES, SAG, NM and KU acknowledge the core services and support from the Lorry I. Lokey Center for Life Science and Engineering. The authors also wish to thank the student Sharon Nissinmann for her help in some of the biosensing experiments. SAG is most grateful for the Russell Berrie Nanotechnology Excellence Scholarship for Graduate Students.
PY - 2020/6/4
Y1 - 2020/6/4
N2 - Over the past decade aptamers have emerged as a promising class of bioreceptors for biosensing applications with significant advantages over conventional antibodies. However, experimental studies comparing aptasensors and immunosensors, under equivalent conditions, are limited and the results are inconclusive, in terms of benefits and limitations of each bioreceptor type. In the present work, the performance of aptamer and antibody bioreceptors for the detection of a his-tagged protein, used as a model target, is compared. The bioreceptors are immobilized onto a nanostructured porous silicon (PSi) thin film, used as the optical transducer, and the target protein is detected in a real-time and label-free format by reflective interferometric Fourier transform spectroscopy. For the antibodies, random-oriented immobilization onto the PSi nanostructure results in a poor biosensing performance. Contrary, Fc-oriented immobilization of the antibodies shows a similar biosensing performance to that exhibited by the aptamer-based biosensor, in terms of binding rate, dynamic detection range, limit of detection and selectivity. The aptasensor outperforms in terms of its reusability and storability, while the immunosensor could not be regenerated for subsequent experiments.
AB - Over the past decade aptamers have emerged as a promising class of bioreceptors for biosensing applications with significant advantages over conventional antibodies. However, experimental studies comparing aptasensors and immunosensors, under equivalent conditions, are limited and the results are inconclusive, in terms of benefits and limitations of each bioreceptor type. In the present work, the performance of aptamer and antibody bioreceptors for the detection of a his-tagged protein, used as a model target, is compared. The bioreceptors are immobilized onto a nanostructured porous silicon (PSi) thin film, used as the optical transducer, and the target protein is detected in a real-time and label-free format by reflective interferometric Fourier transform spectroscopy. For the antibodies, random-oriented immobilization onto the PSi nanostructure results in a poor biosensing performance. Contrary, Fc-oriented immobilization of the antibodies shows a similar biosensing performance to that exhibited by the aptamer-based biosensor, in terms of binding rate, dynamic detection range, limit of detection and selectivity. The aptasensor outperforms in terms of its reusability and storability, while the immunosensor could not be regenerated for subsequent experiments.
UR - http://www.scopus.com/inward/record.url?scp=85088487464&partnerID=8YFLogxK
U2 - 10.1039/d0an00178c
DO - 10.1039/d0an00178c
M3 - Article
C2 - 32519701
AN - SCOPUS:85088487464
VL - 145
SP - 4991
EP - 5003
JO - The analyst
JF - The analyst
SN - 0003-2654
IS - 14
ER -