Details
| Original language | English |
|---|---|
| Pages (from-to) | 7097-7109 |
| Number of pages | 13 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 97 |
| Issue number | 16 |
| Publication status | Published - 10 Jul 2013 |
Abstract
Aptamers are nucleic acid oligomers with distinct conformational shapes that allow them to bind targets with high affinity and specificity. Aptamers are selected from a random oligonucleotide library by their capability to bind a certain molecular target. A variety of targets ranging from small molecules like amino acids to complex targets and whole cells have been used to select aptamers. These characteristics and the ability to create specific aptamers against virtually any cell type in a process termed "systematic evolution by exponential enrichment" make them interesting tools for flow cytometry. In this contribution, we review the application of aptamers as probes for flow cytometry, especially cell-phenotyping and detection of various cancer cell lines and virus-infected cells and pathogens. We also discuss the potential of aptamers combined with nanoparticles such as quantum dots for the generation of new multivalent detector molecules with enhanced affinity and sensitivity. With regard to recent advancements in aptamer selection and the decreasing costs for oligonucleotide synthesis, aptamers may rise as potent competitors for antibodies as molecular probes in flow cytometry.
Keywords
- Aptamer-modified nanoparticles, Aptamer-modified quantum dots, Aptamers, Cell-SELEX, Flow cytometry, Multivalent aptamers
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
Sustainable Development Goals
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In: Applied Microbiology and Biotechnology, Vol. 97, No. 16, 10.07.2013, p. 7097-7109.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Aptamers: versatile probes for flow cytometry
AU - Meyer, Michael
AU - Scheper, Thomas
AU - Walter, Johanna Gabriela
PY - 2013/7/10
Y1 - 2013/7/10
N2 - Aptamers are nucleic acid oligomers with distinct conformational shapes that allow them to bind targets with high affinity and specificity. Aptamers are selected from a random oligonucleotide library by their capability to bind a certain molecular target. A variety of targets ranging from small molecules like amino acids to complex targets and whole cells have been used to select aptamers. These characteristics and the ability to create specific aptamers against virtually any cell type in a process termed "systematic evolution by exponential enrichment" make them interesting tools for flow cytometry. In this contribution, we review the application of aptamers as probes for flow cytometry, especially cell-phenotyping and detection of various cancer cell lines and virus-infected cells and pathogens. We also discuss the potential of aptamers combined with nanoparticles such as quantum dots for the generation of new multivalent detector molecules with enhanced affinity and sensitivity. With regard to recent advancements in aptamer selection and the decreasing costs for oligonucleotide synthesis, aptamers may rise as potent competitors for antibodies as molecular probes in flow cytometry.
AB - Aptamers are nucleic acid oligomers with distinct conformational shapes that allow them to bind targets with high affinity and specificity. Aptamers are selected from a random oligonucleotide library by their capability to bind a certain molecular target. A variety of targets ranging from small molecules like amino acids to complex targets and whole cells have been used to select aptamers. These characteristics and the ability to create specific aptamers against virtually any cell type in a process termed "systematic evolution by exponential enrichment" make them interesting tools for flow cytometry. In this contribution, we review the application of aptamers as probes for flow cytometry, especially cell-phenotyping and detection of various cancer cell lines and virus-infected cells and pathogens. We also discuss the potential of aptamers combined with nanoparticles such as quantum dots for the generation of new multivalent detector molecules with enhanced affinity and sensitivity. With regard to recent advancements in aptamer selection and the decreasing costs for oligonucleotide synthesis, aptamers may rise as potent competitors for antibodies as molecular probes in flow cytometry.
KW - Aptamer-modified nanoparticles
KW - Aptamer-modified quantum dots
KW - Aptamers
KW - Cell-SELEX
KW - Flow cytometry
KW - Multivalent aptamers
UR - http://www.scopus.com/inward/record.url?scp=84881248780&partnerID=8YFLogxK
U2 - 10.1007/s00253-013-5070-z
DO - 10.1007/s00253-013-5070-z
M3 - Review article
C2 - 23838792
AN - SCOPUS:84881248780
VL - 97
SP - 7097
EP - 7109
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 16
ER -