Details
| Original language | English |
|---|---|
| Pages (from-to) | 496-506 |
| Number of pages | 11 |
| Journal | Engineering in life sciences |
| Volume | 12 |
| Issue number | 5 |
| Publication status | Published - 25 Jun 2012 |
Abstract
Aptamers are single-stranded synthetic oligonucleotides that are able to capture their target molecule with high affinity and specificity. Therefore, they can be thought of as nucleic acid-based alternative to antibodies, which have several advantages over their amino acid-based counterparts. Consequently, aptamers can be used in different applications based on molecular recognition including affinity separations. This review will summarize the state-of-the-art in aptamer-based affinity separations; will discuss the current limitations and will highlight possible future prospects. The first part will point out the advantages of aptamers in downstream processing. Here, the properties of aptamers will be discussed along with their implications on downstream processing from a user's point of view. In the second part, a brief summary of the literature is given with focus on aptamer-based separation of proteins. Finally, some drawbacks of aptamers will be illustrated and possibilities to overcome these limitations will be suggested. New technologies in the fields of aptamer selection and synthesis are expected to further promote the use of aptamers as affinity ligands in downstream processing.
Keywords
- Affinity separation, Aptamer, Chromatography, Downstream processing
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Environmental Science(all)
- Environmental Engineering
- Chemical Engineering(all)
- Bioengineering
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In: Engineering in life sciences, Vol. 12, No. 5, 25.06.2012, p. 496-506.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Aptamers as affinity ligands for downstream processing
AU - Walter, Johanna Gabriela
AU - Stahl, Frank
AU - Scheper, Thomas
PY - 2012/6/25
Y1 - 2012/6/25
N2 - Aptamers are single-stranded synthetic oligonucleotides that are able to capture their target molecule with high affinity and specificity. Therefore, they can be thought of as nucleic acid-based alternative to antibodies, which have several advantages over their amino acid-based counterparts. Consequently, aptamers can be used in different applications based on molecular recognition including affinity separations. This review will summarize the state-of-the-art in aptamer-based affinity separations; will discuss the current limitations and will highlight possible future prospects. The first part will point out the advantages of aptamers in downstream processing. Here, the properties of aptamers will be discussed along with their implications on downstream processing from a user's point of view. In the second part, a brief summary of the literature is given with focus on aptamer-based separation of proteins. Finally, some drawbacks of aptamers will be illustrated and possibilities to overcome these limitations will be suggested. New technologies in the fields of aptamer selection and synthesis are expected to further promote the use of aptamers as affinity ligands in downstream processing.
AB - Aptamers are single-stranded synthetic oligonucleotides that are able to capture their target molecule with high affinity and specificity. Therefore, they can be thought of as nucleic acid-based alternative to antibodies, which have several advantages over their amino acid-based counterparts. Consequently, aptamers can be used in different applications based on molecular recognition including affinity separations. This review will summarize the state-of-the-art in aptamer-based affinity separations; will discuss the current limitations and will highlight possible future prospects. The first part will point out the advantages of aptamers in downstream processing. Here, the properties of aptamers will be discussed along with their implications on downstream processing from a user's point of view. In the second part, a brief summary of the literature is given with focus on aptamer-based separation of proteins. Finally, some drawbacks of aptamers will be illustrated and possibilities to overcome these limitations will be suggested. New technologies in the fields of aptamer selection and synthesis are expected to further promote the use of aptamers as affinity ligands in downstream processing.
KW - Affinity separation
KW - Aptamer
KW - Chromatography
KW - Downstream processing
UR - http://www.scopus.com/inward/record.url?scp=84867908015&partnerID=8YFLogxK
U2 - 10.1002/elsc.201100197
DO - 10.1002/elsc.201100197
M3 - Review article
AN - SCOPUS:84867908015
VL - 12
SP - 496
EP - 506
JO - Engineering in life sciences
JF - Engineering in life sciences
SN - 1618-0240
IS - 5
ER -