Details
| Original language | English |
|---|---|
| Pages (from-to) | 43-51 |
| Number of pages | 9 |
| Journal | BioNanoMaterials |
| Volume | 17 |
| Issue number | 1-2 |
| Early online date | 27 Apr 2016 |
| Publication status | Published - 1 May 2016 |
Abstract
The purpose of this study was to develop a model system for targeted drug delivery. This system should enable targeted drug release at a certain tissue in the body. In conventional drug delivery systems, drugs are often delivered unspecifically resulting in unwarranted adverse effects. To circumvent this problem, there is an increasing demand for the development of intelligent drug delivery systems allowing a tissue-specific mode of delivery. Within this study, nanoparticles consisting of two biocompatible polymers are used. Because of their small size, nanoparticles are well-suited for effective drug delivery. The small size affects their movement through cell and tissue barriers. Their cellular uptake is easier when compared to larger drug delivery systems. Paclitaxel was encapsulated into the nanoparticles as a model drug, and to achieve specific targeting an aptamer directed against lung cancer cells was coupled to the nanoparticles surface. Nanoparticles were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), and nanotracking analysis (NTA). Also their surface charge was characterized from ζ-potential measurements. Their preparation was optimized and subsequently specificity of drug-loaded and aptamer-functionalized nanoparticles was investigated using lung cancer cells.
Keywords
- aptamer, HOOC-PEG-PCL, mPEG-PCL, nanoparticles
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Engineering(all)
- Biomedical Engineering
Sustainable Development Goals
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In: BioNanoMaterials, Vol. 17, No. 1-2, 01.05.2016, p. 43-51.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Aptamer-modified polymer nanoparticles for targeted drug delivery
AU - Modrejewski, Julia
AU - Walter, Johanna Gabriela
AU - Kretschmer, Imme
AU - Kemal, Evren
AU - Green, Mark
AU - Belhadj, Hamza
AU - Blume, Cornelia
AU - Scheper, Thomas
N1 - Julia Modrejewski would like to acknowledge Hannover School for Biomolecular Drug Research (HSBDR) for financial support.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - The purpose of this study was to develop a model system for targeted drug delivery. This system should enable targeted drug release at a certain tissue in the body. In conventional drug delivery systems, drugs are often delivered unspecifically resulting in unwarranted adverse effects. To circumvent this problem, there is an increasing demand for the development of intelligent drug delivery systems allowing a tissue-specific mode of delivery. Within this study, nanoparticles consisting of two biocompatible polymers are used. Because of their small size, nanoparticles are well-suited for effective drug delivery. The small size affects their movement through cell and tissue barriers. Their cellular uptake is easier when compared to larger drug delivery systems. Paclitaxel was encapsulated into the nanoparticles as a model drug, and to achieve specific targeting an aptamer directed against lung cancer cells was coupled to the nanoparticles surface. Nanoparticles were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), and nanotracking analysis (NTA). Also their surface charge was characterized from ζ-potential measurements. Their preparation was optimized and subsequently specificity of drug-loaded and aptamer-functionalized nanoparticles was investigated using lung cancer cells.
AB - The purpose of this study was to develop a model system for targeted drug delivery. This system should enable targeted drug release at a certain tissue in the body. In conventional drug delivery systems, drugs are often delivered unspecifically resulting in unwarranted adverse effects. To circumvent this problem, there is an increasing demand for the development of intelligent drug delivery systems allowing a tissue-specific mode of delivery. Within this study, nanoparticles consisting of two biocompatible polymers are used. Because of their small size, nanoparticles are well-suited for effective drug delivery. The small size affects their movement through cell and tissue barriers. Their cellular uptake is easier when compared to larger drug delivery systems. Paclitaxel was encapsulated into the nanoparticles as a model drug, and to achieve specific targeting an aptamer directed against lung cancer cells was coupled to the nanoparticles surface. Nanoparticles were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), and nanotracking analysis (NTA). Also their surface charge was characterized from ζ-potential measurements. Their preparation was optimized and subsequently specificity of drug-loaded and aptamer-functionalized nanoparticles was investigated using lung cancer cells.
KW - aptamer
KW - HOOC-PEG-PCL
KW - mPEG-PCL
KW - nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84991518355&partnerID=8YFLogxK
U2 - 10.1515/bnm-2015-0027
DO - 10.1515/bnm-2015-0027
M3 - Article
AN - SCOPUS:84991518355
VL - 17
SP - 43
EP - 51
JO - BioNanoMaterials
JF - BioNanoMaterials
SN - 2193-0651
IS - 1-2
ER -