Details
| Original language | English |
|---|---|
| Pages (from-to) | 67-82 |
| Number of pages | 16 |
| Journal | Methods in Molecular Biology |
| Volume | 1286 |
| Publication status | Published - 2015 |
Abstract
Aptamers are valuable alternative ligands for affinity separations. Here, we describe the aptamer-based affinity separation of His-tagged proteins using an aptamer directed against the His-tag. The immobilization of the aptamer to magnetic beads is described as well as the aptamer-based purification and proper methods for the characterization of the process. Moreover, indications for the transfer of the process to other aptamers are given.
Keywords
- Affinity separation, Aptamer, His-tag, Magnetic beads, Protein purification
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
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In: Methods in Molecular Biology, Vol. 1286, 2015, p. 67-82.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Aptamer-modified magnetic beads in affinity separation of proteins
AU - Zhu, Guohong
AU - Walter, Johanna Gabriela
PY - 2015
Y1 - 2015
N2 - Aptamers are valuable alternative ligands for affinity separations. Here, we describe the aptamer-based affinity separation of His-tagged proteins using an aptamer directed against the His-tag. The immobilization of the aptamer to magnetic beads is described as well as the aptamer-based purification and proper methods for the characterization of the process. Moreover, indications for the transfer of the process to other aptamers are given.
AB - Aptamers are valuable alternative ligands for affinity separations. Here, we describe the aptamer-based affinity separation of His-tagged proteins using an aptamer directed against the His-tag. The immobilization of the aptamer to magnetic beads is described as well as the aptamer-based purification and proper methods for the characterization of the process. Moreover, indications for the transfer of the process to other aptamers are given.
KW - Affinity separation
KW - Aptamer
KW - His-tag
KW - Magnetic beads
KW - Protein purification
UR - http://www.scopus.com/inward/record.url?scp=84924406226&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-2447-9_7
DO - 10.1007/978-1-4939-2447-9_7
M3 - Article
C2 - 25749947
AN - SCOPUS:84924406226
VL - 1286
SP - 67
EP - 82
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -