Details
| Original language | English |
|---|---|
| Journal | AIMS Bioengineering |
| Volume | 5 |
| Issue number | 2 |
| Publication status | Published - 23 Apr 2018 |
Abstract
Aptamer-based lateral flow assays (LFAs) are an emerging field of aptamer applications due to numerous potential applications. When compared to antibodies, potential advantages like cost effectiveness or lower batch to batch variations are evident. The development of LFAs for small molecules, however, is still challenging due to several reasons, primarily linked to target size and accessible interaction sites. In small molecule analysis, however, aptamers in many cases are preferable since immunogenicity is not required and they may exhibit even higher target selectivity. We report the first cross-recognition of a small molecule (ampicillin) and a protein (C-reactive protein), predicted by in-silico analysis, then experimentally confirmed - using two different aptamers. These features can be exploited for developing an aptamer-based LFA for label-free ampicillin detection, functioning also for analysis in milk extract. Most importantly, the principal setup denotes a novel, transferable and versatile general approach for detection of small molecules using competitive LFAs, unlikely to be generally realized by aptamer-DNA-binding otherwise.
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In: AIMS Bioengineering, Vol. 5, No. 2, 23.04.2018.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Aptamer-based lateral flow assays
AU - Schüling, Torsten
AU - Eilers, Alina
AU - Scheper, Thomas
AU - Walter, Johanna
N1 - Funding information: This work was partially supported by the Federal Ministry of Education and Research of Germany (MultiFlow, Grant 03FH046PX4) and by a scholarship for Lars Kaiser from the Federal Ministry of Science, Research and Art of Baden-Württemberg. Text editing by Julien Landre, PhD, Munich, is gratefully acknowledged.
PY - 2018/4/23
Y1 - 2018/4/23
N2 - Aptamer-based lateral flow assays (LFAs) are an emerging field of aptamer applications due to numerous potential applications. When compared to antibodies, potential advantages like cost effectiveness or lower batch to batch variations are evident. The development of LFAs for small molecules, however, is still challenging due to several reasons, primarily linked to target size and accessible interaction sites. In small molecule analysis, however, aptamers in many cases are preferable since immunogenicity is not required and they may exhibit even higher target selectivity. We report the first cross-recognition of a small molecule (ampicillin) and a protein (C-reactive protein), predicted by in-silico analysis, then experimentally confirmed - using two different aptamers. These features can be exploited for developing an aptamer-based LFA for label-free ampicillin detection, functioning also for analysis in milk extract. Most importantly, the principal setup denotes a novel, transferable and versatile general approach for detection of small molecules using competitive LFAs, unlikely to be generally realized by aptamer-DNA-binding otherwise.
AB - Aptamer-based lateral flow assays (LFAs) are an emerging field of aptamer applications due to numerous potential applications. When compared to antibodies, potential advantages like cost effectiveness or lower batch to batch variations are evident. The development of LFAs for small molecules, however, is still challenging due to several reasons, primarily linked to target size and accessible interaction sites. In small molecule analysis, however, aptamers in many cases are preferable since immunogenicity is not required and they may exhibit even higher target selectivity. We report the first cross-recognition of a small molecule (ampicillin) and a protein (C-reactive protein), predicted by in-silico analysis, then experimentally confirmed - using two different aptamers. These features can be exploited for developing an aptamer-based LFA for label-free ampicillin detection, functioning also for analysis in milk extract. Most importantly, the principal setup denotes a novel, transferable and versatile general approach for detection of small molecules using competitive LFAs, unlikely to be generally realized by aptamer-DNA-binding otherwise.
U2 - 10.3934/bioeng.2018.2.78
DO - 10.3934/bioeng.2018.2.78
M3 - Article
VL - 5
JO - AIMS Bioengineering
JF - AIMS Bioengineering
SN - 2375-1495
IS - 2
ER -