Details
| Original language | English |
|---|---|
| Pages (from-to) | 2371-2379 |
| Number of pages | 9 |
| Journal | Biotechnology and Bioengineering |
| Volume | 108 |
| Issue number | 10 |
| Publication status | Published - 2 May 2011 |
Abstract
Aptamers are synthetic nucleic acid-based high affinity ligands that are able to capture their corresponding target via molecular recognition. Here, aptamer-based affinity purification for His-tagged proteins was developed. Two different aptamers directed against the His-tag were immobilized on magnetic beads covalently. The resulting aptamer-modified magnetic beads were characterized and successfully applied for purification of different His-tagged proteins from complex E. coli cell lysates. Purification effects comparable to conventional immobilized metal affinity chromatography were achieved in one single purification step. Moreover, we have investigated the possibility to regenerate and reuse the aptamer-modified magnetic beads and have shown their long-term stability over a period of 6 months.
Keywords
- Aptamer, Downstream processing, Magnetic beads
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Biotechnology and Bioengineering, Vol. 108, No. 10, 02.05.2011, p. 2371-2379.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Aptamer-Based Downstream Processing ofHis-Tagged Proteins Utilizing Magnetic Beads
AU - Kökpinar, Öznur
AU - Walter, Johanna Gabriela
AU - Shoham, Yuval
AU - Stahl, Frank
AU - Scheper, Thomas
PY - 2011/5/2
Y1 - 2011/5/2
N2 - Aptamers are synthetic nucleic acid-based high affinity ligands that are able to capture their corresponding target via molecular recognition. Here, aptamer-based affinity purification for His-tagged proteins was developed. Two different aptamers directed against the His-tag were immobilized on magnetic beads covalently. The resulting aptamer-modified magnetic beads were characterized and successfully applied for purification of different His-tagged proteins from complex E. coli cell lysates. Purification effects comparable to conventional immobilized metal affinity chromatography were achieved in one single purification step. Moreover, we have investigated the possibility to regenerate and reuse the aptamer-modified magnetic beads and have shown their long-term stability over a period of 6 months.
AB - Aptamers are synthetic nucleic acid-based high affinity ligands that are able to capture their corresponding target via molecular recognition. Here, aptamer-based affinity purification for His-tagged proteins was developed. Two different aptamers directed against the His-tag were immobilized on magnetic beads covalently. The resulting aptamer-modified magnetic beads were characterized and successfully applied for purification of different His-tagged proteins from complex E. coli cell lysates. Purification effects comparable to conventional immobilized metal affinity chromatography were achieved in one single purification step. Moreover, we have investigated the possibility to regenerate and reuse the aptamer-modified magnetic beads and have shown their long-term stability over a period of 6 months.
KW - Aptamer
KW - Downstream processing
KW - Magnetic beads
UR - http://www.scopus.com/inward/record.url?scp=80051802390&partnerID=8YFLogxK
U2 - 10.1002/bit.23191
DO - 10.1002/bit.23191
M3 - Article
C2 - 21538335
AN - SCOPUS:80051802390
VL - 108
SP - 2371
EP - 2379
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 10
ER -