Details
| Original language | English |
|---|---|
| Title of host publication | Three-Dimensional and Multidimensional Microscopy |
| Subtitle of host publication | Image Acquisition and Processing XVII |
| Publication status | Published - 24 Feb 2010 |
| Externally published | Yes |
| Event | Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII - San Francisco, CA, United States Duration: 25 Jan 2010 → 28 Jan 2010 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 7570 |
| ISSN (Print) | 1605-7422 |
Abstract
Light microscopy is one of the major tools in modern biology. The steady development of new microscopic techniques leads to an correspondent improvement of biological methods. To expand the catalog of biological experiments, we investigate the possibilities of optical projection tomography (OPT). This technique is based on the already established X-Ray computed tomography. In contrast to most other three-dimensional microscopy techniques it is able to create three dimensional data sets of the specimens natural absorption, staining and fluorescence. Unfortunately, these advantages are opposed by a low resolution, reconstruction artifacts, and a relatively big loss of fluorescence light. We reduced the disadvantage in resolution by applying physical filters in the Fourier plane of the image path, which is not possible in X-Ray imaging yet.
Keywords
- Annular aperture, Bessel beam, Focal depth, Focal length, Optical projection tomography, Resolution, Ring aperture, Super resolution
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Materials Science(all)
- Biomaterials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Cite this
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Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII. 2010. 75700F (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7570).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Applying optical Fourier filtering to standard optical projection tomography
AU - Lorbeer, Raoul Amadeus
AU - Meyer, Heiko
AU - Heidrich, Marko
AU - Lubatschowski, Holger
AU - Heisterkamp, Alexander
PY - 2010/2/24
Y1 - 2010/2/24
N2 - Light microscopy is one of the major tools in modern biology. The steady development of new microscopic techniques leads to an correspondent improvement of biological methods. To expand the catalog of biological experiments, we investigate the possibilities of optical projection tomography (OPT). This technique is based on the already established X-Ray computed tomography. In contrast to most other three-dimensional microscopy techniques it is able to create three dimensional data sets of the specimens natural absorption, staining and fluorescence. Unfortunately, these advantages are opposed by a low resolution, reconstruction artifacts, and a relatively big loss of fluorescence light. We reduced the disadvantage in resolution by applying physical filters in the Fourier plane of the image path, which is not possible in X-Ray imaging yet.
AB - Light microscopy is one of the major tools in modern biology. The steady development of new microscopic techniques leads to an correspondent improvement of biological methods. To expand the catalog of biological experiments, we investigate the possibilities of optical projection tomography (OPT). This technique is based on the already established X-Ray computed tomography. In contrast to most other three-dimensional microscopy techniques it is able to create three dimensional data sets of the specimens natural absorption, staining and fluorescence. Unfortunately, these advantages are opposed by a low resolution, reconstruction artifacts, and a relatively big loss of fluorescence light. We reduced the disadvantage in resolution by applying physical filters in the Fourier plane of the image path, which is not possible in X-Ray imaging yet.
KW - Annular aperture
KW - Bessel beam
KW - Focal depth
KW - Focal length
KW - Optical projection tomography
KW - Resolution
KW - Ring aperture
KW - Super resolution
UR - http://www.scopus.com/inward/record.url?scp=77951870913&partnerID=8YFLogxK
U2 - 10.1117/12.841599
DO - 10.1117/12.841599
M3 - Conference contribution
AN - SCOPUS:77951870913
SN - 9780819479662
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Three-Dimensional and Multidimensional Microscopy
T2 - Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII
Y2 - 25 January 2010 through 28 January 2010
ER -