Details
| Original language | English |
|---|---|
| Pages (from-to) | 126-131 |
| Number of pages | 6 |
| Journal | Biotechnology progress |
| Volume | 12 |
| Issue number | 1 |
| Publication status | Published - 1996 |
Abstract
Conventional observation of a fermentation process by using NAD(P)H-dependent fluorescence provides a mean for cell monitoring, but its practical application has been very limited due to many factors that affect culture fluorescence. In this work we studied correlation of scanning fluorometry signals with various process variables and with cell metabolic states and showed that scanning fluorometry is more informative than conventional NAD(P)H-dependent fluorescence. A stepwise multiple-regression procedure has been used to select meaningful signals from the whole spectrum, and we found that with three or four components of the excitation-emission plane the process variables can be portrayed with a rather good accuracy. The approach is demonstrated on the examples of wild-type or recombinant Escherichia coli and Saccharomyces cerevisiae fermentations.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
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In: Biotechnology progress, Vol. 12, No. 1, 1996, p. 126-131.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Application of Scanning Fluorometry for Monitoring of a Fermentation Process
AU - Tartakovsky, B.
AU - Sheintuch, M.
AU - Hilmer, J. M.
AU - Scheper, T.
PY - 1996
Y1 - 1996
N2 - Conventional observation of a fermentation process by using NAD(P)H-dependent fluorescence provides a mean for cell monitoring, but its practical application has been very limited due to many factors that affect culture fluorescence. In this work we studied correlation of scanning fluorometry signals with various process variables and with cell metabolic states and showed that scanning fluorometry is more informative than conventional NAD(P)H-dependent fluorescence. A stepwise multiple-regression procedure has been used to select meaningful signals from the whole spectrum, and we found that with three or four components of the excitation-emission plane the process variables can be portrayed with a rather good accuracy. The approach is demonstrated on the examples of wild-type or recombinant Escherichia coli and Saccharomyces cerevisiae fermentations.
AB - Conventional observation of a fermentation process by using NAD(P)H-dependent fluorescence provides a mean for cell monitoring, but its practical application has been very limited due to many factors that affect culture fluorescence. In this work we studied correlation of scanning fluorometry signals with various process variables and with cell metabolic states and showed that scanning fluorometry is more informative than conventional NAD(P)H-dependent fluorescence. A stepwise multiple-regression procedure has been used to select meaningful signals from the whole spectrum, and we found that with three or four components of the excitation-emission plane the process variables can be portrayed with a rather good accuracy. The approach is demonstrated on the examples of wild-type or recombinant Escherichia coli and Saccharomyces cerevisiae fermentations.
UR - http://www.scopus.com/inward/record.url?scp=0030019292&partnerID=8YFLogxK
U2 - 10.1021/bp950045h
DO - 10.1021/bp950045h
M3 - Article
C2 - 8845103
AN - SCOPUS:0030019292
VL - 12
SP - 126
EP - 131
JO - Biotechnology progress
JF - Biotechnology progress
SN - 8756-7938
IS - 1
ER -