Details
| Original language | English |
|---|---|
| Title of host publication | Optical Methods for Industrial Processes |
| Pages | 50-57 |
| Number of pages | 8 |
| Volume | 4201 |
| Publication status | Published - 13 Feb 2001 |
Publication series
| Name | Proceedings of SPIE - The International Society for Optical Engineering |
|---|---|
| Publisher | SPIE |
| ISSN (Print) | 0277-786X |
Abstract
Modern bioprocess control requires fast data acquisition and in-time evaluation of bioprocess variables. On-line fluorescence spectroscopy for data acquisition and the use of chemometric methods accomplish these requirements. The presented investigations were performed with fluorescence spectrometers with wide ranges of excitation and emission wavelength. By detection of several biogenic fluorophors (amino acids, coenzyms and vitamins) a large amount of information about the state of the bioprocess are obtained. For the evaluation of the process variables partial least squares regression (PLS) is used. This technique was applied to several bioprocesses: the production of ergotamine by Claviceps purpurea, the production of t-PA (tissue plasminogen activator) by animal cells and brewing processes. The main point of monitoring the brewing processes was to determine the process variables cell count and extract concentration.
Keywords
- Bioprocess monitoring, Brewing process, Fluorescence spectroscopy, Multivariate analysis, On-line
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Condensed Matter Physics
- Computer Science(all)
- Computer Science Applications
- Mathematics(all)
- Applied Mathematics
- Engineering(all)
- Electrical and Electronic Engineering
Cite this
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Optical Methods for Industrial Processes. Vol. 4201 2001. p. 50-57 (Proceedings of SPIE - The International Society for Optical Engineering).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Application of fluorescence spectroscopy for on-line bioprocess monitoring and control
AU - Boehl, D.
AU - Solle, D.
AU - Toussaint, H. J.
AU - Menge, M.
AU - Renemann, G.
AU - Lindemann, C.
AU - Hitzmann, B.
AU - Scheper, T.
PY - 2001/2/13
Y1 - 2001/2/13
N2 - Modern bioprocess control requires fast data acquisition and in-time evaluation of bioprocess variables. On-line fluorescence spectroscopy for data acquisition and the use of chemometric methods accomplish these requirements. The presented investigations were performed with fluorescence spectrometers with wide ranges of excitation and emission wavelength. By detection of several biogenic fluorophors (amino acids, coenzyms and vitamins) a large amount of information about the state of the bioprocess are obtained. For the evaluation of the process variables partial least squares regression (PLS) is used. This technique was applied to several bioprocesses: the production of ergotamine by Claviceps purpurea, the production of t-PA (tissue plasminogen activator) by animal cells and brewing processes. The main point of monitoring the brewing processes was to determine the process variables cell count and extract concentration.
AB - Modern bioprocess control requires fast data acquisition and in-time evaluation of bioprocess variables. On-line fluorescence spectroscopy for data acquisition and the use of chemometric methods accomplish these requirements. The presented investigations were performed with fluorescence spectrometers with wide ranges of excitation and emission wavelength. By detection of several biogenic fluorophors (amino acids, coenzyms and vitamins) a large amount of information about the state of the bioprocess are obtained. For the evaluation of the process variables partial least squares regression (PLS) is used. This technique was applied to several bioprocesses: the production of ergotamine by Claviceps purpurea, the production of t-PA (tissue plasminogen activator) by animal cells and brewing processes. The main point of monitoring the brewing processes was to determine the process variables cell count and extract concentration.
KW - Bioprocess monitoring
KW - Brewing process
KW - Fluorescence spectroscopy
KW - Multivariate analysis
KW - On-line
UR - http://www.scopus.com/inward/record.url?scp=0035030865&partnerID=8YFLogxK
U2 - 10.1117/12.417395
DO - 10.1117/12.417395
M3 - Conference contribution
AN - SCOPUS:0035030865
VL - 4201
T3 - Proceedings of SPIE - The International Society for Optical Engineering
SP - 50
EP - 57
BT - Optical Methods for Industrial Processes
ER -