Details
| Original language | English |
|---|---|
| Pages (from-to) | 263-269 |
| Number of pages | 7 |
| Journal | Analytica chimica acta |
| Volume | 296 |
| Issue number | 3 |
| Publication status | Published - 20 Oct 1994 |
| Externally published | Yes |
Abstract
Enzymes were (co)immobilized on the pH-sensitive gates of an eight-channel field-effect transistor (FET). Glucose was analysed with a glucose dehydrogenase (GDH) FET, maltose with a coimmobilized maltase (MAL)/GDH FET, sucrose with a coimmobilized invertase (INV)/GDH FET, lactose with a β-galactosidase (β-GAL)/galactose dehydrogenase (GALDH)-fusion protein FET and ethanol with a coimmobilized alcohol dehydrogenase (ADH)/aldehyde dehyrogenase (ALDH) FET. These EnFETs were integrated in FIA systems, calibrated and characterized with respect to buffer capacity, pH value, stirrer speed and NAD concentration and cross sensitivity. On account of the pH and buffer capacity sensitivity of the signals of the EnFETs, the pH value was monitored with a pH-FET of the array and the buffer capacity and substrate concentration were calculated from the shape of the signal of the FIA system. The EnFETs were stored at 4°C for many months without activity loss. They have satisfactory activity for performing a large number of analyses.
Keywords
- EnFET, Enzymatic methods, Field effect transistors, Flow injection, Sensors
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Environmental Science(all)
- Environmental Chemistry
- Chemistry(all)
- Spectroscopy
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In: Analytica chimica acta, Vol. 296, No. 3, 20.10.1994, p. 263-269.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Application of enzyme field-effect transistor sensor arrays as detectors in a flow-injection system for simultaneous monitoring of medium components. Part I. Preparation and calibration.
AU - Kullick, T.
AU - Beyer, M.
AU - Henning, J.
AU - Lerch, T.
AU - Quack, R.
AU - Zeitz, A.
AU - Hitzmann, B.
AU - Scheper, T.
AU - Schürgerl, K.
N1 - Funding information: &GAL/GALDH and thank the Ministry of Re-searcho f Technologyo f the Federal Republic of Germany,B onn, for the financial support.
PY - 1994/10/20
Y1 - 1994/10/20
N2 - Enzymes were (co)immobilized on the pH-sensitive gates of an eight-channel field-effect transistor (FET). Glucose was analysed with a glucose dehydrogenase (GDH) FET, maltose with a coimmobilized maltase (MAL)/GDH FET, sucrose with a coimmobilized invertase (INV)/GDH FET, lactose with a β-galactosidase (β-GAL)/galactose dehydrogenase (GALDH)-fusion protein FET and ethanol with a coimmobilized alcohol dehydrogenase (ADH)/aldehyde dehyrogenase (ALDH) FET. These EnFETs were integrated in FIA systems, calibrated and characterized with respect to buffer capacity, pH value, stirrer speed and NAD concentration and cross sensitivity. On account of the pH and buffer capacity sensitivity of the signals of the EnFETs, the pH value was monitored with a pH-FET of the array and the buffer capacity and substrate concentration were calculated from the shape of the signal of the FIA system. The EnFETs were stored at 4°C for many months without activity loss. They have satisfactory activity for performing a large number of analyses.
AB - Enzymes were (co)immobilized on the pH-sensitive gates of an eight-channel field-effect transistor (FET). Glucose was analysed with a glucose dehydrogenase (GDH) FET, maltose with a coimmobilized maltase (MAL)/GDH FET, sucrose with a coimmobilized invertase (INV)/GDH FET, lactose with a β-galactosidase (β-GAL)/galactose dehydrogenase (GALDH)-fusion protein FET and ethanol with a coimmobilized alcohol dehydrogenase (ADH)/aldehyde dehyrogenase (ALDH) FET. These EnFETs were integrated in FIA systems, calibrated and characterized with respect to buffer capacity, pH value, stirrer speed and NAD concentration and cross sensitivity. On account of the pH and buffer capacity sensitivity of the signals of the EnFETs, the pH value was monitored with a pH-FET of the array and the buffer capacity and substrate concentration were calculated from the shape of the signal of the FIA system. The EnFETs were stored at 4°C for many months without activity loss. They have satisfactory activity for performing a large number of analyses.
KW - EnFET
KW - Enzymatic methods
KW - Field effect transistors
KW - Flow injection
KW - Sensors
UR - http://www.scopus.com/inward/record.url?scp=0028018835&partnerID=8YFLogxK
U2 - 10.1016/0003-2670(94)80245-9
DO - 10.1016/0003-2670(94)80245-9
M3 - Article
AN - SCOPUS:0028018835
VL - 296
SP - 263
EP - 269
JO - Analytica chimica acta
JF - Analytica chimica acta
SN - 0003-2670
IS - 3
ER -