Details
| Original language | English |
|---|---|
| Pages (from-to) | 175-180 |
| Number of pages | 6 |
| Journal | Process biochemistry |
| Volume | 33 |
| Issue number | 2 |
| Publication status | Published - 29 May 1998 |
Abstract
A fluoride sensitive (pF), buffer capacity insensitive electrolyte isolator semiconductor capacitor (pF-EIS-CAP) chip was used as a transducer for biosensors with co-immobilized enzymes. Hydrogen peroxide forms fluoride ions in stoichiometric amounts in the presence of p-fluoraniline and peroxidase (POD). This was measured by the pF-EIS-CAP-sensor. The combination of this sensor with various oxidases allows the measurement of the concentration of several analytes (Beyer et al., Biosensors and Bioelectronics, 1994, 9, 17-21; Menzel et al., Analyt. Chim. Acta, 1995, 317, 259-264). Sugar syrup (glucose, maltose, maltotriose and other oligosaccharide) concentrations were analysed with co-immobilized β-amylase, amyloglucosidase, mutarotase, glucose oxidase and peroxidase. The glucose concentration was analysed with co-immobilized glucose oxidase and peroxidase, the ethanol concentration with co-immobilized alcohol oxidase and peroxidase and the phosphate concentration by co-immobilized nucleoside phosphorylase, xanthin oxidase and peroxidase. These (Bio-pF-EIS-CAP) sensors were integrated in flow injection analysis systems. The medium components were monitored on-line during Saccharomyces cerevisiae, Acremonium chrysogenum and recombinant baby hamster kidney (rBHK) cell cultivations. The agreement between the off- and on-line concentrations of the medium components was within 10%.
Keywords
- β- amylase, Alcohol oxidase, Amyloglucosidase, Fluorid sensor, Glucose oxidase, Mutarotase, Nucleotide phosphorylase, Peroxidase, Xanthine oxidase
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Process biochemistry, Vol. 33, No. 2, 29.05.1998, p. 175-180.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Application of biosensors with an electrolyte isolator semiconductor capacitor (EIS-CAP) transducer for process monitoring
AU - Menzel, C.
AU - Lerch, T.
AU - Schneider, K.
AU - Weidemann, R.
AU - Tollnick, C.
AU - Kretzmer, G.
AU - Scheper, T.
AU - Schügerl, K.
N1 - Funding information: The authors gratefully acknowledge the financial support of the German Scientific Foundation (DFG) and the Federal Ministry of Research and Technology (BMFT Project No. 0310087A).
PY - 1998/5/29
Y1 - 1998/5/29
N2 - A fluoride sensitive (pF), buffer capacity insensitive electrolyte isolator semiconductor capacitor (pF-EIS-CAP) chip was used as a transducer for biosensors with co-immobilized enzymes. Hydrogen peroxide forms fluoride ions in stoichiometric amounts in the presence of p-fluoraniline and peroxidase (POD). This was measured by the pF-EIS-CAP-sensor. The combination of this sensor with various oxidases allows the measurement of the concentration of several analytes (Beyer et al., Biosensors and Bioelectronics, 1994, 9, 17-21; Menzel et al., Analyt. Chim. Acta, 1995, 317, 259-264). Sugar syrup (glucose, maltose, maltotriose and other oligosaccharide) concentrations were analysed with co-immobilized β-amylase, amyloglucosidase, mutarotase, glucose oxidase and peroxidase. The glucose concentration was analysed with co-immobilized glucose oxidase and peroxidase, the ethanol concentration with co-immobilized alcohol oxidase and peroxidase and the phosphate concentration by co-immobilized nucleoside phosphorylase, xanthin oxidase and peroxidase. These (Bio-pF-EIS-CAP) sensors were integrated in flow injection analysis systems. The medium components were monitored on-line during Saccharomyces cerevisiae, Acremonium chrysogenum and recombinant baby hamster kidney (rBHK) cell cultivations. The agreement between the off- and on-line concentrations of the medium components was within 10%.
AB - A fluoride sensitive (pF), buffer capacity insensitive electrolyte isolator semiconductor capacitor (pF-EIS-CAP) chip was used as a transducer for biosensors with co-immobilized enzymes. Hydrogen peroxide forms fluoride ions in stoichiometric amounts in the presence of p-fluoraniline and peroxidase (POD). This was measured by the pF-EIS-CAP-sensor. The combination of this sensor with various oxidases allows the measurement of the concentration of several analytes (Beyer et al., Biosensors and Bioelectronics, 1994, 9, 17-21; Menzel et al., Analyt. Chim. Acta, 1995, 317, 259-264). Sugar syrup (glucose, maltose, maltotriose and other oligosaccharide) concentrations were analysed with co-immobilized β-amylase, amyloglucosidase, mutarotase, glucose oxidase and peroxidase. The glucose concentration was analysed with co-immobilized glucose oxidase and peroxidase, the ethanol concentration with co-immobilized alcohol oxidase and peroxidase and the phosphate concentration by co-immobilized nucleoside phosphorylase, xanthin oxidase and peroxidase. These (Bio-pF-EIS-CAP) sensors were integrated in flow injection analysis systems. The medium components were monitored on-line during Saccharomyces cerevisiae, Acremonium chrysogenum and recombinant baby hamster kidney (rBHK) cell cultivations. The agreement between the off- and on-line concentrations of the medium components was within 10%.
KW - β- amylase
KW - Alcohol oxidase
KW - Amyloglucosidase
KW - Fluorid sensor
KW - Glucose oxidase
KW - Mutarotase
KW - Nucleotide phosphorylase
KW - Peroxidase
KW - Xanthine oxidase
UR - http://www.scopus.com/inward/record.url?scp=0032006719&partnerID=8YFLogxK
U2 - 10.1016/S0032-9592(97)00044-7
DO - 10.1016/S0032-9592(97)00044-7
M3 - Article
AN - SCOPUS:0032006719
VL - 33
SP - 175
EP - 180
JO - Process biochemistry
JF - Process biochemistry
SN - 1359-5113
IS - 2
ER -