Details
| Original language | English |
|---|---|
| Pages (from-to) | 29-32 |
| Number of pages | 4 |
| Journal | Biosensors and Bioelectronics |
| Volume | 9 |
| Issue number | 1 |
| Publication status | Published - 1994 |
| Externally published | Yes |
Abstract
The analysis of different amino acids is described using a fibre optic biosensor previously described in its principles. This biosensor works according to the measurement principle of flow injection analysis (FIA) and consists of a fluorosensor and a measurement cell operating under pressure. In the measurement cell, enzymes and molecular weight enlarged coenzyme PEG (MW 20000)-N6-(2-aminoethyl)-NAD(H) are confined behind a solid ultrafiltration membrane. Assays for the analysis of L-phenylalanine and L-alanine were developed. The analysis frequency is in the range of 1 to 2 samples per hour, and the sensor stability was found to be sensitive to the stability of each enzyme system used. This effect was studied in detail. The L-alanine assay was found to be especially reliable, sensitive, specific, and highly selective for the L-enantiomer.
Keywords
- enzyme/coenzyme immobilization, FIA, fibre optic biosensor, molecular weight enlarged PEG-NAD(H)., NADH-dependent assays
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Engineering(all)
- Biomedical Engineering
- Chemistry(all)
- Electrochemistry
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In: Biosensors and Bioelectronics, Vol. 9, No. 1, 1994, p. 29-32.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Application of a flow injection fibre optic biosensor for the analysis of different amino acids
AU - Lee, S. J.
AU - Scheper, Th
AU - Bückmann, A. F.
PY - 1994
Y1 - 1994
N2 - The analysis of different amino acids is described using a fibre optic biosensor previously described in its principles. This biosensor works according to the measurement principle of flow injection analysis (FIA) and consists of a fluorosensor and a measurement cell operating under pressure. In the measurement cell, enzymes and molecular weight enlarged coenzyme PEG (MW 20000)-N6-(2-aminoethyl)-NAD(H) are confined behind a solid ultrafiltration membrane. Assays for the analysis of L-phenylalanine and L-alanine were developed. The analysis frequency is in the range of 1 to 2 samples per hour, and the sensor stability was found to be sensitive to the stability of each enzyme system used. This effect was studied in detail. The L-alanine assay was found to be especially reliable, sensitive, specific, and highly selective for the L-enantiomer.
AB - The analysis of different amino acids is described using a fibre optic biosensor previously described in its principles. This biosensor works according to the measurement principle of flow injection analysis (FIA) and consists of a fluorosensor and a measurement cell operating under pressure. In the measurement cell, enzymes and molecular weight enlarged coenzyme PEG (MW 20000)-N6-(2-aminoethyl)-NAD(H) are confined behind a solid ultrafiltration membrane. Assays for the analysis of L-phenylalanine and L-alanine were developed. The analysis frequency is in the range of 1 to 2 samples per hour, and the sensor stability was found to be sensitive to the stability of each enzyme system used. This effect was studied in detail. The L-alanine assay was found to be especially reliable, sensitive, specific, and highly selective for the L-enantiomer.
KW - enzyme/coenzyme immobilization
KW - FIA
KW - fibre optic biosensor
KW - molecular weight enlarged PEG-NAD(H).
KW - NADH-dependent assays
UR - http://www.scopus.com/inward/record.url?scp=0028265598&partnerID=8YFLogxK
U2 - 10.1016/0956-5663(94)80012-X
DO - 10.1016/0956-5663(94)80012-X
M3 - Article
C2 - 8166955
AN - SCOPUS:0028265598
VL - 9
SP - 29
EP - 32
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
IS - 1
ER -