Details
| Original language | English |
|---|---|
| Pages (from-to) | 6184-6189 |
| Number of pages | 6 |
| Journal | Analytical chemistry |
| Volume | 77 |
| Issue number | 19 |
| Publication status | Published - 23 Aug 2005 |
Abstract
2D-Fluorescence spectroscopy has been shown to be effective for the on-line monitoring of spectroscopic detectable substrates L-phenylalanine-7-amido-4- methylcoumarine (L-PheAMC) and D-phenylalanine-7-amido-4- trifluoromethylcoumarine (D-PheAFC) in supercritical carbon dioxide. Earlier investigations with the coumarine substrates in watery and organic phases showed their potential for on-line enantiomeric evaluations of enzymatic reactions in different reaction media. The solubility of the different substrates and their fluorescence maximums were investigated in SCCO2. The sole hydrolyzations of L-PheAMC and D-PheAFC with α-chymotrypsin and the esterase from porcine liver were tracked on-line in the supercritical medium; however, different solubility characteristics of the methyl- and trifluoromethyl-substituted coumarins influence the simultaneous detection of the L- and D-substrate within the applied high-pressure reactor system.
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
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In: Analytical chemistry, Vol. 77, No. 19, 23.08.2005, p. 6184-6189.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Application of 2D-Fluorescence Spectroscopy for On-Line Monitoring of Pseudoenantiomeric Transformations in Supercritical Carbon Dioxide Systems
AU - Knüttel, Torsten
AU - Meyer, Hartmut
AU - Scheper, Thomas
PY - 2005/8/23
Y1 - 2005/8/23
N2 - 2D-Fluorescence spectroscopy has been shown to be effective for the on-line monitoring of spectroscopic detectable substrates L-phenylalanine-7-amido-4- methylcoumarine (L-PheAMC) and D-phenylalanine-7-amido-4- trifluoromethylcoumarine (D-PheAFC) in supercritical carbon dioxide. Earlier investigations with the coumarine substrates in watery and organic phases showed their potential for on-line enantiomeric evaluations of enzymatic reactions in different reaction media. The solubility of the different substrates and their fluorescence maximums were investigated in SCCO2. The sole hydrolyzations of L-PheAMC and D-PheAFC with α-chymotrypsin and the esterase from porcine liver were tracked on-line in the supercritical medium; however, different solubility characteristics of the methyl- and trifluoromethyl-substituted coumarins influence the simultaneous detection of the L- and D-substrate within the applied high-pressure reactor system.
AB - 2D-Fluorescence spectroscopy has been shown to be effective for the on-line monitoring of spectroscopic detectable substrates L-phenylalanine-7-amido-4- methylcoumarine (L-PheAMC) and D-phenylalanine-7-amido-4- trifluoromethylcoumarine (D-PheAFC) in supercritical carbon dioxide. Earlier investigations with the coumarine substrates in watery and organic phases showed their potential for on-line enantiomeric evaluations of enzymatic reactions in different reaction media. The solubility of the different substrates and their fluorescence maximums were investigated in SCCO2. The sole hydrolyzations of L-PheAMC and D-PheAFC with α-chymotrypsin and the esterase from porcine liver were tracked on-line in the supercritical medium; however, different solubility characteristics of the methyl- and trifluoromethyl-substituted coumarins influence the simultaneous detection of the L- and D-substrate within the applied high-pressure reactor system.
UR - http://www.scopus.com/inward/record.url?scp=26444459962&partnerID=8YFLogxK
U2 - 10.1021/ac050747d
DO - 10.1021/ac050747d
M3 - Article
C2 - 16194077
AN - SCOPUS:26444459962
VL - 77
SP - 6184
EP - 6189
JO - Analytical chemistry
JF - Analytical chemistry
SN - 0003-2700
IS - 19
ER -