Details
| Original language | English |
|---|---|
| Pages (from-to) | 14-16 |
| Number of pages | 3 |
| Journal | Archiv fur Lebensmittelhygiene |
| Volume | 48 |
| Issue number | 1 |
| Publication status | Published - 1997 |
Abstract
In this study, 64 chicken skin samples and 126 breast samples were examined concerning the correlation between measurements of microbial ATP with the bioluminescent method that allows to examine samples with a lower degree of contamination than the common bioluminescent method because it offers the possibility of drawing the 10th quantity of sample through the filter by vacuum and thereby concentrating the number of microorganisms. The samples were purchased from local supermarkets and farmer's markets and prepared for all determinations by swabbing a defined area of their surface. The swab sampling technique was compared with a destructive method. The results indicate that the difference between the values obtained by swabbing and the total number of CFU is relatively constant (r = 0.93). The difference is about a half tenth power. Parallel to the ATP measurement, the microbial level of all samples was determined on plate count. Additionally, the Enterobacteriaceae colony count was determined for the breast samples. The limits of detection were > 102 CFU per cm2 for chicken skin and > 102 CFU per cm2 for the breast samples with correlation coefficients of r = 0.95 and r = 0.89 respectively. Furthermore, a correlation between CFU and the Enterobacteriaceae colony count was found.
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Food Science
- Immunology and Microbiology(all)
- Microbiology
- Environmental Science(all)
- Management, Monitoring, Policy and Law
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In: Archiv fur Lebensmittelhygiene, Vol. 48, No. 1, 1997, p. 14-16.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Applicability of the swab sampling technique in order to determine the microbial quality of poultry by means of ATP Bioluminescence
AU - Werlein, H. D.
AU - Fricke, Regina
PY - 1997
Y1 - 1997
N2 - In this study, 64 chicken skin samples and 126 breast samples were examined concerning the correlation between measurements of microbial ATP with the bioluminescent method that allows to examine samples with a lower degree of contamination than the common bioluminescent method because it offers the possibility of drawing the 10th quantity of sample through the filter by vacuum and thereby concentrating the number of microorganisms. The samples were purchased from local supermarkets and farmer's markets and prepared for all determinations by swabbing a defined area of their surface. The swab sampling technique was compared with a destructive method. The results indicate that the difference between the values obtained by swabbing and the total number of CFU is relatively constant (r = 0.93). The difference is about a half tenth power. Parallel to the ATP measurement, the microbial level of all samples was determined on plate count. Additionally, the Enterobacteriaceae colony count was determined for the breast samples. The limits of detection were > 102 CFU per cm2 for chicken skin and > 102 CFU per cm2 for the breast samples with correlation coefficients of r = 0.95 and r = 0.89 respectively. Furthermore, a correlation between CFU and the Enterobacteriaceae colony count was found.
AB - In this study, 64 chicken skin samples and 126 breast samples were examined concerning the correlation between measurements of microbial ATP with the bioluminescent method that allows to examine samples with a lower degree of contamination than the common bioluminescent method because it offers the possibility of drawing the 10th quantity of sample through the filter by vacuum and thereby concentrating the number of microorganisms. The samples were purchased from local supermarkets and farmer's markets and prepared for all determinations by swabbing a defined area of their surface. The swab sampling technique was compared with a destructive method. The results indicate that the difference between the values obtained by swabbing and the total number of CFU is relatively constant (r = 0.93). The difference is about a half tenth power. Parallel to the ATP measurement, the microbial level of all samples was determined on plate count. Additionally, the Enterobacteriaceae colony count was determined for the breast samples. The limits of detection were > 102 CFU per cm2 for chicken skin and > 102 CFU per cm2 for the breast samples with correlation coefficients of r = 0.95 and r = 0.89 respectively. Furthermore, a correlation between CFU and the Enterobacteriaceae colony count was found.
UR - http://www.scopus.com/inward/record.url?scp=0031475276&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0031475276
VL - 48
SP - 14
EP - 16
JO - Archiv fur Lebensmittelhygiene
JF - Archiv fur Lebensmittelhygiene
SN - 0003-925X
IS - 1
ER -