Details
| Original language | English |
|---|---|
| Pages (from-to) | 2560-2566 |
| Number of pages | 7 |
| Journal | ACS infectious diseases |
| Volume | 6 |
| Issue number | 10 |
| Early online date | 15 Sept 2020 |
| Publication status | Published - 9 Oct 2020 |
Abstract
There is a demonstrated and paramount need for rapid, reliable infectious disease diagnostics, particularly those for invasive fungal infections. Current clinical determinations for an appropriate antifungal therapy can take up to 3 days using current antifungal susceptibility testing methods, a time-to-readout that can prove detrimental for immunocompromised patients and promote the spread of antifungal resistant pathogens. Herein, we demonstrate the application of intensity-based reflectometric interference spectroscopic measurements (termed iPRISM) on microstructured silicon sensors for use as a rapid, phenotypic antifungal susceptibility test. This diagnostic platform optically tracks morphological changes of fungi corresponding to conidia growth and hyphal colonization at a solid-liquid interface in real time. Using Aspergillus niger as a model fungal pathogen, we can determine the minimal inhibitory concentration of clinically relevant antifungals within 12 h. This assay allows for expedited detection of fungal growth and provides a label-free alternative to broth microdilution and agar diffusion methods, with the potential to be used for point-of-care diagnostics.
Keywords
- antifungal susceptibility testing, Aspergillus Niger, fungal resistance, optical sensor, sensor
ASJC Scopus subject areas
- Medicine(all)
- Infectious Diseases
Sustainable Development Goals
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In: ACS infectious diseases, Vol. 6, No. 10, 09.10.2020, p. 2560-2566.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Antifungal Susceptibility Testing of Aspergillus Niger on Silicon Microwells by Intensity-Based Reflectometric Interference Spectroscopy
AU - Heuer, Christopher
AU - Leonard, Heidi
AU - Nitzan, Nadav
AU - Lavy-Alperovitch, Ariella
AU - Massad-Ivanir, Naama
AU - Scheper, Thomas
AU - Segal, Ester
N1 - Funding Information: This work was partially supported by the Israel Innovation Authority (Kamin program). C.H. acknowledges financial aid from the Leibniz Universitätsgesellschaft and the Technion. We thank Dima Peselev and Orna Ternyak (MNFPU, Technion) for the microfabrication of the photonic chips and Omer Sabach for his artwork depicting the microfluidic channels (A).
PY - 2020/10/9
Y1 - 2020/10/9
N2 - There is a demonstrated and paramount need for rapid, reliable infectious disease diagnostics, particularly those for invasive fungal infections. Current clinical determinations for an appropriate antifungal therapy can take up to 3 days using current antifungal susceptibility testing methods, a time-to-readout that can prove detrimental for immunocompromised patients and promote the spread of antifungal resistant pathogens. Herein, we demonstrate the application of intensity-based reflectometric interference spectroscopic measurements (termed iPRISM) on microstructured silicon sensors for use as a rapid, phenotypic antifungal susceptibility test. This diagnostic platform optically tracks morphological changes of fungi corresponding to conidia growth and hyphal colonization at a solid-liquid interface in real time. Using Aspergillus niger as a model fungal pathogen, we can determine the minimal inhibitory concentration of clinically relevant antifungals within 12 h. This assay allows for expedited detection of fungal growth and provides a label-free alternative to broth microdilution and agar diffusion methods, with the potential to be used for point-of-care diagnostics.
AB - There is a demonstrated and paramount need for rapid, reliable infectious disease diagnostics, particularly those for invasive fungal infections. Current clinical determinations for an appropriate antifungal therapy can take up to 3 days using current antifungal susceptibility testing methods, a time-to-readout that can prove detrimental for immunocompromised patients and promote the spread of antifungal resistant pathogens. Herein, we demonstrate the application of intensity-based reflectometric interference spectroscopic measurements (termed iPRISM) on microstructured silicon sensors for use as a rapid, phenotypic antifungal susceptibility test. This diagnostic platform optically tracks morphological changes of fungi corresponding to conidia growth and hyphal colonization at a solid-liquid interface in real time. Using Aspergillus niger as a model fungal pathogen, we can determine the minimal inhibitory concentration of clinically relevant antifungals within 12 h. This assay allows for expedited detection of fungal growth and provides a label-free alternative to broth microdilution and agar diffusion methods, with the potential to be used for point-of-care diagnostics.
KW - antifungal susceptibility testing
KW - Aspergillus Niger
KW - fungal resistance
KW - optical sensor
KW - sensor
UR - http://www.scopus.com/inward/record.url?scp=85092750049&partnerID=8YFLogxK
U2 - 10.1021/acsinfecdis.0c00234
DO - 10.1021/acsinfecdis.0c00234
M3 - Article
C2 - 32930571
AN - SCOPUS:85092750049
VL - 6
SP - 2560
EP - 2566
JO - ACS infectious diseases
JF - ACS infectious diseases
SN - 2373-8227
IS - 10
ER -