Details
| Original language | English |
|---|---|
| Article number | e1800632 |
| Number of pages | 12 |
| Journal | Chemistry and Biodiversity |
| Volume | 16 |
| Issue number | 5 |
| Publication status | Published - May 2019 |
Abstract
In the search of new compounds with biofilm-inhibiting properties, mangroves with their richness of secondary metabolites can be a valuable resource. Crude methanolic leaf extracts from the mangrove Laguncularia racemosa enriched in phenolic substances cause a reduction in initial cell adhesion of Candida glabrata and Candida albicans, but not on Escherichia coli. LC/MS-guided fractionation of the phenolic compounds resulted in 19 fractions, of which ten were analyzed for their bioactivity against cell adhesion. Effects on cell adhesion and planktonic growth of Escherichia coli, Candida glabrata and Candida albicans were measured in 96-well microtiter plates in the presence of 0.2 mg ml−1 of the isolated fractions. Two fractions caused a reduction of cell adhesion of Candida albicans. These fractions containing bioactive compounds were analyzed by LC/MS and NMR spectroscopy. Casuarinin and digalloyl-hexahydroxydiphenoyl-glucose were identified in the active fractions, in addition to three signals of ellagitannins. These results indicate a specific mode of action of hydrolysable tannins against cell adhesion of Candida albicans, which needs to be further analyzed.
Keywords
- bioactive compounds, biological activity, Laguncularia racemosa, mangrove, phenolic compounds, phytochemistry, tannins
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Chemistry(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Medicine
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
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In: Chemistry and Biodiversity, Vol. 16, No. 5, e1800632, 05.2019.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Anti-Adhesion Activity of Tannins Isolated from the Mangrove Laguncularia racemosa
AU - Glasenapp, Yvana
AU - Lucas, Christina
AU - Wöltje, Timo
AU - Fohrer, Jörg
AU - Papenbrock, Jutta
N1 - Funding information: We would like to thank Pamela von Trzebiatowski for her assistance with the execution of cell adhesion and antimicrobial susceptibility tests and Yvonne Leye and Fabian Söffker for taking care of the mangrove plants. The project was partially funded by the Hannover School for Biomolecular Drug Research (HSBDR) financed by the state of Lower Saxony and supported by the German-Italian bilateral project ‘Bioactive secondary compounds from halophyte species inhibit biofilm formation of plant-pathogenic microorganisms on plant surfaces’ (SAB-HAL), MIUR-DAAD Joint Mobility Program, ID-57265315.
PY - 2019/5
Y1 - 2019/5
N2 - In the search of new compounds with biofilm-inhibiting properties, mangroves with their richness of secondary metabolites can be a valuable resource. Crude methanolic leaf extracts from the mangrove Laguncularia racemosa enriched in phenolic substances cause a reduction in initial cell adhesion of Candida glabrata and Candida albicans, but not on Escherichia coli. LC/MS-guided fractionation of the phenolic compounds resulted in 19 fractions, of which ten were analyzed for their bioactivity against cell adhesion. Effects on cell adhesion and planktonic growth of Escherichia coli, Candida glabrata and Candida albicans were measured in 96-well microtiter plates in the presence of 0.2 mg ml−1 of the isolated fractions. Two fractions caused a reduction of cell adhesion of Candida albicans. These fractions containing bioactive compounds were analyzed by LC/MS and NMR spectroscopy. Casuarinin and digalloyl-hexahydroxydiphenoyl-glucose were identified in the active fractions, in addition to three signals of ellagitannins. These results indicate a specific mode of action of hydrolysable tannins against cell adhesion of Candida albicans, which needs to be further analyzed.
AB - In the search of new compounds with biofilm-inhibiting properties, mangroves with their richness of secondary metabolites can be a valuable resource. Crude methanolic leaf extracts from the mangrove Laguncularia racemosa enriched in phenolic substances cause a reduction in initial cell adhesion of Candida glabrata and Candida albicans, but not on Escherichia coli. LC/MS-guided fractionation of the phenolic compounds resulted in 19 fractions, of which ten were analyzed for their bioactivity against cell adhesion. Effects on cell adhesion and planktonic growth of Escherichia coli, Candida glabrata and Candida albicans were measured in 96-well microtiter plates in the presence of 0.2 mg ml−1 of the isolated fractions. Two fractions caused a reduction of cell adhesion of Candida albicans. These fractions containing bioactive compounds were analyzed by LC/MS and NMR spectroscopy. Casuarinin and digalloyl-hexahydroxydiphenoyl-glucose were identified in the active fractions, in addition to three signals of ellagitannins. These results indicate a specific mode of action of hydrolysable tannins against cell adhesion of Candida albicans, which needs to be further analyzed.
KW - bioactive compounds
KW - biological activity
KW - Laguncularia racemosa
KW - mangrove
KW - phenolic compounds
KW - phytochemistry
KW - tannins
UR - http://www.scopus.com/inward/record.url?scp=85064596642&partnerID=8YFLogxK
U2 - 10.1002/cbdv.201800632
DO - 10.1002/cbdv.201800632
M3 - Article
C2 - 30835942
AN - SCOPUS:85064596642
VL - 16
JO - Chemistry and Biodiversity
JF - Chemistry and Biodiversity
SN - 1612-1872
IS - 5
M1 - e1800632
ER -