Details
| Original language | English |
|---|---|
| Pages (from-to) | 171-176 |
| Number of pages | 6 |
| Journal | Jurnal Teknologi |
| Volume | 77 |
| Issue number | 24 |
| Publication status | Published - 1 Jan 2015 |
| Externally published | Yes |
Abstract
Mycosphaerella graminicola is an important pathogenic fungus of the wheat that causes Septoria tritici blotch. The genome of M. graminicola has been sequence and each domain of all polyketide synthase enzymes (PKS) was analysed and compared with known sequences from NCBI database. NCBI Basic Local Alignment Search Tool (BLAST) analysis and alignment using Vector NTi software suggested that M. graminicola has 10 PKS and 1 PKSNRPS (nonribosomal peptide synthetase) hybrid, which could be involved in pathogenicity. Out of the 10 polyketide synthase genes, two are non-reducing PKS (NRPKS) and eight are annotated as highly reducing PKS (HRPKS). The NRPKS was labelled as MgPKS1 and MgPKS9. The HR PKS were labelled as MgPKS2, MgPKS3, MgPKS4, MgPKS5, MgPKS6, MgPKS7, and MgPKS8. The PKS-NRPS also have a highly reducing PKS as part of the hybrid.
Keywords
- Mycosphaerella graminicola, Polyketide, Polyketide synthase, Polyketide-nonribosomal peptide synthetase, Septoria leaf blotch
ASJC Scopus subject areas
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In: Jurnal Teknologi, Vol. 77, No. 24, 01.01.2015, p. 171-176.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Annotations and domain analysis of polyketide synthase genes in fungal wheat pathogen, mycosphaerella graminicola
AU - Khalid, Rozida Mohd
AU - Bailey, Andy
AU - Cox, R. J.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Mycosphaerella graminicola is an important pathogenic fungus of the wheat that causes Septoria tritici blotch. The genome of M. graminicola has been sequence and each domain of all polyketide synthase enzymes (PKS) was analysed and compared with known sequences from NCBI database. NCBI Basic Local Alignment Search Tool (BLAST) analysis and alignment using Vector NTi software suggested that M. graminicola has 10 PKS and 1 PKSNRPS (nonribosomal peptide synthetase) hybrid, which could be involved in pathogenicity. Out of the 10 polyketide synthase genes, two are non-reducing PKS (NRPKS) and eight are annotated as highly reducing PKS (HRPKS). The NRPKS was labelled as MgPKS1 and MgPKS9. The HR PKS were labelled as MgPKS2, MgPKS3, MgPKS4, MgPKS5, MgPKS6, MgPKS7, and MgPKS8. The PKS-NRPS also have a highly reducing PKS as part of the hybrid.
AB - Mycosphaerella graminicola is an important pathogenic fungus of the wheat that causes Septoria tritici blotch. The genome of M. graminicola has been sequence and each domain of all polyketide synthase enzymes (PKS) was analysed and compared with known sequences from NCBI database. NCBI Basic Local Alignment Search Tool (BLAST) analysis and alignment using Vector NTi software suggested that M. graminicola has 10 PKS and 1 PKSNRPS (nonribosomal peptide synthetase) hybrid, which could be involved in pathogenicity. Out of the 10 polyketide synthase genes, two are non-reducing PKS (NRPKS) and eight are annotated as highly reducing PKS (HRPKS). The NRPKS was labelled as MgPKS1 and MgPKS9. The HR PKS were labelled as MgPKS2, MgPKS3, MgPKS4, MgPKS5, MgPKS6, MgPKS7, and MgPKS8. The PKS-NRPS also have a highly reducing PKS as part of the hybrid.
KW - Mycosphaerella graminicola
KW - Polyketide
KW - Polyketide synthase
KW - Polyketide-nonribosomal peptide synthetase
KW - Septoria leaf blotch
UR - http://www.scopus.com/inward/record.url?scp=84949810679&partnerID=8YFLogxK
U2 - 10.11113/jt.v77.6727
DO - 10.11113/jt.v77.6727
M3 - Article
AN - SCOPUS:84949810679
VL - 77
SP - 171
EP - 176
JO - Jurnal Teknologi
JF - Jurnal Teknologi
SN - 0127-9696
IS - 24
ER -