Details
| Original language | English |
|---|---|
| Article number | 4097 |
| Pages (from-to) | 4097-4111 |
| Number of pages | 15 |
| Journal | Biomedical optics express |
| Volume | 14 |
| Issue number | 8 |
| Early online date | 12 Jul 2023 |
| Publication status | Published - 1 Aug 2023 |
Abstract
The structure of the SARS-CoV-2 spike RBD and human ACE2 as well as changes in the structure due to binding activities were analysed using surface enhanced Raman spectroscopy. The inhibitor cohaerin C was applied to inhibit the binding between spike RBD and ACE2. Differences and changes in the Raman spectra were determined using deconvolution of the amide bands and principal component analysis. We thus demonstrate a fast and label-free analysis of the protein structures and the differentiation between bound and unbound states. The approach is suitable for sensing and screening and might be relevant to investigate other protein systems as well.
ASJC Scopus subject areas
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
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In: Biomedical optics express, Vol. 14, No. 8, 4097, 01.08.2023, p. 4097-4111.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Analysis of SARS-CoV-2 spike RBD binding to ACE2 and its inhibition by fungal cohaerin C using surface enhanced Raman spectroscopy
AU - Wetzel, Christoph
AU - Jansen-Olliges, Linda
AU - Stadler, Marc
AU - Surup, Frank
AU - Zeilinger, Carsten
AU - Roth, Bernhard
N1 - Publisher Copyright: © 2023 OSA - The Optical Society. All rights reserved.
PY - 2023/8/1
Y1 - 2023/8/1
N2 - The structure of the SARS-CoV-2 spike RBD and human ACE2 as well as changes in the structure due to binding activities were analysed using surface enhanced Raman spectroscopy. The inhibitor cohaerin C was applied to inhibit the binding between spike RBD and ACE2. Differences and changes in the Raman spectra were determined using deconvolution of the amide bands and principal component analysis. We thus demonstrate a fast and label-free analysis of the protein structures and the differentiation between bound and unbound states. The approach is suitable for sensing and screening and might be relevant to investigate other protein systems as well.
AB - The structure of the SARS-CoV-2 spike RBD and human ACE2 as well as changes in the structure due to binding activities were analysed using surface enhanced Raman spectroscopy. The inhibitor cohaerin C was applied to inhibit the binding between spike RBD and ACE2. Differences and changes in the Raman spectra were determined using deconvolution of the amide bands and principal component analysis. We thus demonstrate a fast and label-free analysis of the protein structures and the differentiation between bound and unbound states. The approach is suitable for sensing and screening and might be relevant to investigate other protein systems as well.
UR - http://www.scopus.com/inward/record.url?scp=85165465433&partnerID=8YFLogxK
U2 - 10.1364/BOE.495685
DO - 10.1364/BOE.495685
M3 - Article
VL - 14
SP - 4097
EP - 4111
JO - Biomedical optics express
JF - Biomedical optics express
SN - 2156-7085
IS - 8
M1 - 4097
ER -