Details
| Original language | English |
|---|---|
| Article number | e0258956 |
| Journal | PLOS ONE |
| Volume | 16 |
| Issue number | 10 October |
| Publication status | Published - 22 Oct 2021 |
Abstract
The genus Halophila shows the highest species diversity within the seagrass genera. Southeast Asian countries where several boundary lines exist were considered as the origin of seagrasses. We hypothesize that the boundary lines, such as Wallace’s and Lydekker’s Lines, may act as marine geographic barriers to the population structure of Halophila major. Seagrass samples were collected at three islands in Vietnamese waters and analyzed by the molecular maker ITS. These sequences were compared with published ITS sequences from seagrasses collected in the whole region of interest. In this study, we reveal the haplotype and nucleotide diversity, linking population genetics, phylogeography, phylogenetics and estimation of relative divergence times of H. major and other members of the Halophila genus. The morphological characters show variation. The results of the ITS marker analysis reveal smaller groups of H. major from Myanmar, Shoalwater Bay (Australia) and Okinawa (Japan) with high supporting values. The remaining groups including Sri Lanka, Viet Nam, the Philippines, Thailand, Malaysia, Indonesia, Two Peoples Bay (Australia) and Tokushima (Japan) showed low supporting values. The Wallacea region shows the highest haplotype and also nucleotide diversity. Non-significant differences were found among regions, but significant differences were presented among populations. The relative divergence times between some members of section Halophila were estimated 2.15–6.64 Mya.
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In: PLOS ONE, Vol. 16, No. 10 October, e0258956, 22.10.2021.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Analysis of rDNA reveals a high genetic diversity of Halophila major in the Wallacea region
AU - Nguyen, Xuan Vy
AU - Nguyen-Nhat, Nhu Thuy
AU - Nguyen, Xuan Thuy
AU - Dao, Viet Ha
AU - Liao, Lawrence M.
AU - Papenbrock, Jutta
N1 - Funding Information: This study was funded by the National Foundation for Science & Technology Development (NAFOSTED), Viet Nam to XVN with grant number: 106.02-2018.313. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
PY - 2021/10/22
Y1 - 2021/10/22
N2 - The genus Halophila shows the highest species diversity within the seagrass genera. Southeast Asian countries where several boundary lines exist were considered as the origin of seagrasses. We hypothesize that the boundary lines, such as Wallace’s and Lydekker’s Lines, may act as marine geographic barriers to the population structure of Halophila major. Seagrass samples were collected at three islands in Vietnamese waters and analyzed by the molecular maker ITS. These sequences were compared with published ITS sequences from seagrasses collected in the whole region of interest. In this study, we reveal the haplotype and nucleotide diversity, linking population genetics, phylogeography, phylogenetics and estimation of relative divergence times of H. major and other members of the Halophila genus. The morphological characters show variation. The results of the ITS marker analysis reveal smaller groups of H. major from Myanmar, Shoalwater Bay (Australia) and Okinawa (Japan) with high supporting values. The remaining groups including Sri Lanka, Viet Nam, the Philippines, Thailand, Malaysia, Indonesia, Two Peoples Bay (Australia) and Tokushima (Japan) showed low supporting values. The Wallacea region shows the highest haplotype and also nucleotide diversity. Non-significant differences were found among regions, but significant differences were presented among populations. The relative divergence times between some members of section Halophila were estimated 2.15–6.64 Mya.
AB - The genus Halophila shows the highest species diversity within the seagrass genera. Southeast Asian countries where several boundary lines exist were considered as the origin of seagrasses. We hypothesize that the boundary lines, such as Wallace’s and Lydekker’s Lines, may act as marine geographic barriers to the population structure of Halophila major. Seagrass samples were collected at three islands in Vietnamese waters and analyzed by the molecular maker ITS. These sequences were compared with published ITS sequences from seagrasses collected in the whole region of interest. In this study, we reveal the haplotype and nucleotide diversity, linking population genetics, phylogeography, phylogenetics and estimation of relative divergence times of H. major and other members of the Halophila genus. The morphological characters show variation. The results of the ITS marker analysis reveal smaller groups of H. major from Myanmar, Shoalwater Bay (Australia) and Okinawa (Japan) with high supporting values. The remaining groups including Sri Lanka, Viet Nam, the Philippines, Thailand, Malaysia, Indonesia, Two Peoples Bay (Australia) and Tokushima (Japan) showed low supporting values. The Wallacea region shows the highest haplotype and also nucleotide diversity. Non-significant differences were found among regions, but significant differences were presented among populations. The relative divergence times between some members of section Halophila were estimated 2.15–6.64 Mya.
UR - http://www.scopus.com/inward/record.url?scp=85117913370&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0258956
DO - 10.1371/journal.pone.0258956
M3 - Article
C2 - 34679102
AN - SCOPUS:85117913370
VL - 16
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 10 October
M1 - e0258956
ER -