Details
| Original language | English |
|---|---|
| Article number | #303310 |
| Pages (from-to) | 4756-4771 |
| Number of pages | 16 |
| Journal | Biomedical Optics Express |
| Volume | 8 |
| Issue number | 10 |
| Publication status | Published - Oct 2017 |
Abstract
Laser-exposed plasmonic substrates permeabilize the plasma membrane of cells when in close contact to deliver cell-impermeable cargo. While studies have determined the cargo delivery efficiency and viability of laser-exposed plasmonic substrates, morphological changes in a cell have not been quantified. We porated myoblast C2C12 cells on a plasmonic pyramid array using a 532-nm laser with 850-ps pulse length and time-lapse fluorescence imaging to quantify cellular changes. We obtain a poration efficiency of 80%, viability of 90%, and a pore radius of 20 nm. We quantified area changes in the plasma membrane attached to the substrate (10% decrease), nucleus (5 - 10% decrease), and cytoplasm (5 - 10% decrease) over 1 h after laser treatment. Cytoskeleton fibers show a change of 50% in the alignment, or coherency, of fibers, which stabilizes after 10 mins. We investigate structural and morphological changes due to the poration process to enable the safe development of this technique for therapeutic applications.
Keywords
- (000.1430) biology and medicine, (190.4870) photothermal effects, (350.4855) optical tweezers or optical manipulation
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
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In: Biomedical Optics Express, Vol. 8, No. 10, #303310, 10.2017, p. 4756-4771.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Analysis of poration-induced changes in cells from laser-activated plasmonic substrates
AU - Saklayen, Nabiha
AU - Kalies, Stefan
AU - Madrid, Marinna
AU - Nuzzo, Valeria
AU - Huber, Marinus
AU - Shen, Weilu
AU - Sinanan-Singh, Jasmine
AU - Heinemann, Dag
AU - Heisterkamp, Alexander
AU - Mazur, Eric
N1 - Funding information: NS, MM, WS, JSS, and EM were supported by the National Science Foundation under contracts PHY-1219334 and PHY-1205465. NS was funded by the Howard Hughes Medical Institute’s International Fellowship and the short-term research fellowship from DAAD. MM was funded by the Graduate Prize Fellowship at Harvard University. SK and AH received funding from the German Research Foundation through the Cluster of Excellence REBIRTH (DFG EXC62/3). Parts of this work were carried out as an integral part of the BIOFABRICATION FOR NIFE Initiative, which is financially supported by the ministry of Lower Saxony and the Volkswagen Stiftung (BIOFABRICATION FOR NIFE: VWZN2860). NN, MM, and EM have an interest in Cellino Biotech.
PY - 2017/10
Y1 - 2017/10
N2 - Laser-exposed plasmonic substrates permeabilize the plasma membrane of cells when in close contact to deliver cell-impermeable cargo. While studies have determined the cargo delivery efficiency and viability of laser-exposed plasmonic substrates, morphological changes in a cell have not been quantified. We porated myoblast C2C12 cells on a plasmonic pyramid array using a 532-nm laser with 850-ps pulse length and time-lapse fluorescence imaging to quantify cellular changes. We obtain a poration efficiency of 80%, viability of 90%, and a pore radius of 20 nm. We quantified area changes in the plasma membrane attached to the substrate (10% decrease), nucleus (5 - 10% decrease), and cytoplasm (5 - 10% decrease) over 1 h after laser treatment. Cytoskeleton fibers show a change of 50% in the alignment, or coherency, of fibers, which stabilizes after 10 mins. We investigate structural and morphological changes due to the poration process to enable the safe development of this technique for therapeutic applications.
AB - Laser-exposed plasmonic substrates permeabilize the plasma membrane of cells when in close contact to deliver cell-impermeable cargo. While studies have determined the cargo delivery efficiency and viability of laser-exposed plasmonic substrates, morphological changes in a cell have not been quantified. We porated myoblast C2C12 cells on a plasmonic pyramid array using a 532-nm laser with 850-ps pulse length and time-lapse fluorescence imaging to quantify cellular changes. We obtain a poration efficiency of 80%, viability of 90%, and a pore radius of 20 nm. We quantified area changes in the plasma membrane attached to the substrate (10% decrease), nucleus (5 - 10% decrease), and cytoplasm (5 - 10% decrease) over 1 h after laser treatment. Cytoskeleton fibers show a change of 50% in the alignment, or coherency, of fibers, which stabilizes after 10 mins. We investigate structural and morphological changes due to the poration process to enable the safe development of this technique for therapeutic applications.
KW - (000.1430) biology and medicine
KW - (190.4870) photothermal effects
KW - (350.4855) optical tweezers or optical manipulation
UR - http://www.scopus.com/inward/record.url?scp=85031022540&partnerID=8YFLogxK
U2 - 10.1364/BOE.8.004756
DO - 10.1364/BOE.8.004756
M3 - Article
AN - SCOPUS:85031022540
VL - 8
SP - 4756
EP - 4771
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 10
M1 - #303310
ER -