Details
| Original language | English |
|---|---|
| Article number | 689 |
| Number of pages | 19 |
| Journal | Cells |
| Volume | 10 |
| Issue number | 3 |
| Publication status | Published - 20 Mar 2021 |
Abstract
Nucleotides fulfill many essential functions in plants. Compared to non-plant systems, these hydrophilic metabolites have not been adequately investigated in plants, especially the less abundant nucleotide species such as deoxyribonucleotides and modified or damaged nucleotides. Until recently, this was mainly due to a lack of adequate methods for in-depth analysis of nucleotides and nucleosides in plants. In this review, we focus on the current state-of-the-art of nucleotide analysis in plants with liquid chromatography coupled to mass spectrometry and describe recent major advances. Tissue disruption, quenching, liquid–liquid and solid-phase extraction, chromatographic strategies, and peculiarities of nucleotides and nucleosides in mass spectrometry are covered. We describe how the different steps of the analytical workflow influence each other, highlight the specific challenges of nucleotide analysis, and outline promising future developments. The metabolite matrix of plants is particularly complex. Therefore, it is likely that nucleotide analysis methods that work for plants can be applied to other organisms as well. Although this review focuses on plants, we also discuss advances in nucleotide analysis from non-plant systems to provide an overview of the analytical techniques available for this challenging class of metabolites.
Keywords
- Hydrophilic interaction liquid chromatography (HILIC) chromatography, Liquid chromatography, Mass spectrometry, Nucleosides, Nucleotides, Plant metabolomics, Plant nucleotide metabolism, Quenching, Solid phase extraction
ASJC Scopus subject areas
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In: Cells, Vol. 10, No. 3, 689, 20.03.2021.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Analysis of Nucleosides and Nucleotides in Plants: An Update on Sample Preparation and LC–MS Techniques
AU - Straube, Henryk
AU - Witte, Claus-Peter
AU - Herde, Marco
PY - 2021/3/20
Y1 - 2021/3/20
N2 - Nucleotides fulfill many essential functions in plants. Compared to non-plant systems, these hydrophilic metabolites have not been adequately investigated in plants, especially the less abundant nucleotide species such as deoxyribonucleotides and modified or damaged nucleotides. Until recently, this was mainly due to a lack of adequate methods for in-depth analysis of nucleotides and nucleosides in plants. In this review, we focus on the current state-of-the-art of nucleotide analysis in plants with liquid chromatography coupled to mass spectrometry and describe recent major advances. Tissue disruption, quenching, liquid–liquid and solid-phase extraction, chromatographic strategies, and peculiarities of nucleotides and nucleosides in mass spectrometry are covered. We describe how the different steps of the analytical workflow influence each other, highlight the specific challenges of nucleotide analysis, and outline promising future developments. The metabolite matrix of plants is particularly complex. Therefore, it is likely that nucleotide analysis methods that work for plants can be applied to other organisms as well. Although this review focuses on plants, we also discuss advances in nucleotide analysis from non-plant systems to provide an overview of the analytical techniques available for this challenging class of metabolites.
AB - Nucleotides fulfill many essential functions in plants. Compared to non-plant systems, these hydrophilic metabolites have not been adequately investigated in plants, especially the less abundant nucleotide species such as deoxyribonucleotides and modified or damaged nucleotides. Until recently, this was mainly due to a lack of adequate methods for in-depth analysis of nucleotides and nucleosides in plants. In this review, we focus on the current state-of-the-art of nucleotide analysis in plants with liquid chromatography coupled to mass spectrometry and describe recent major advances. Tissue disruption, quenching, liquid–liquid and solid-phase extraction, chromatographic strategies, and peculiarities of nucleotides and nucleosides in mass spectrometry are covered. We describe how the different steps of the analytical workflow influence each other, highlight the specific challenges of nucleotide analysis, and outline promising future developments. The metabolite matrix of plants is particularly complex. Therefore, it is likely that nucleotide analysis methods that work for plants can be applied to other organisms as well. Although this review focuses on plants, we also discuss advances in nucleotide analysis from non-plant systems to provide an overview of the analytical techniques available for this challenging class of metabolites.
KW - Hydrophilic interaction liquid chromatography (HILIC) chromatography
KW - Liquid chromatography
KW - Mass spectrometry
KW - Nucleosides
KW - Nucleotides
KW - Plant metabolomics
KW - Plant nucleotide metabolism
KW - Quenching
KW - Solid phase extraction
UR - http://www.scopus.com/inward/record.url?scp=85103919885&partnerID=8YFLogxK
U2 - 10.3390/cells10030689
DO - 10.3390/cells10030689
M3 - Review article
VL - 10
JO - Cells
JF - Cells
SN - 2073-4409
IS - 3
M1 - 689
ER -