Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Peihong Fang
  • Paul Arens
  • Xintong Liu
  • Xin Zhang
  • Deepika Lakwani
  • Fabrice Foucher
  • Jérémy Clotault
  • Juliane Geike
  • Helgard Kaufmann
  • Thomas Debener
  • Yuling Bai
  • Zhao Zhang
  • Marinus J.M. Smulders

External Research Organisations

  • Wageningen University and Research
  • China Agricultural University
  • University of Angers
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Details

Original languageEnglish
Pages (from-to)2495-2515
Number of pages21
JournalTheoretical and applied genetics
Volume134
Issue number8
Early online date2 May 2021
Publication statusPublished - Aug 2021

Abstract

Key message: Rose has 19 MLO genes. Of these, RhMLO1 and RhMLO2 were shown to be required for powdery mildew infection, which suggests their potential as susceptibility targets towards disease resistance. Abstract: Powdery mildew, caused by Podosphaera pannosa, is one of the most serious and widespread fungal diseases for roses, especially in greenhouse-grown cut roses. It has been shown that certain MLO genes are involved in powdery mildew susceptibility and that loss of function in these genes in various crops leads to broad-spectrum, long-lasting resistance against this fungal disease. For this reason, these MLO genes are called susceptibility genes. We carried out a genome-wide identification of the MLO gene family in the Rosa chinensis genome, and screened for allelic variants among 22 accessions from seven different Rosa species using re-sequencing and transcriptome data. We identified 19 MLO genes in rose, of which four are candidate genes for functional homologs in clade V, which is the clade containing all dicot MLO susceptibility genes. We detected a total of 198 different allelic variants in the set of Rosa species and accessions, corresponding to 5–15 different alleles for each of the genes. Some diploid Rosa species shared alleles with tetraploid rose cultivars, consistent with the notion that diploid species have contributed to the formation of tetraploid roses. Among the four RhMLO genes in clade V, we demonstrated using expression study, virus-induced gene silencing as well as transient RNAi silencing that two of them, RhMLO1 and RhMLO2, are required for infection by P. pannosa and suggest their potential as susceptibility targets for powdery mildew resistance breeding in rose.

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Cite this

Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs. / Fang, Peihong; Arens, Paul; Liu, Xintong et al.
In: Theoretical and applied genetics, Vol. 134, No. 8, 08.2021, p. 2495-2515.

Research output: Contribution to journalArticleResearchpeer review

Fang, P, Arens, P, Liu, X, Zhang, X, Lakwani, D, Foucher, F, Clotault, J, Geike, J, Kaufmann, H, Debener, T, Bai, Y, Zhang, Z & Smulders, MJM 2021, 'Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs', Theoretical and applied genetics, vol. 134, no. 8, pp. 2495-2515. https://doi.org/10.1007/s00122-021-03838-7
Fang, P., Arens, P., Liu, X., Zhang, X., Lakwani, D., Foucher, F., Clotault, J., Geike, J., Kaufmann, H., Debener, T., Bai, Y., Zhang, Z., & Smulders, M. J. M. (2021). Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs. Theoretical and applied genetics, 134(8), 2495-2515. https://doi.org/10.1007/s00122-021-03838-7
Fang P, Arens P, Liu X, Zhang X, Lakwani D, Foucher F et al. Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs. Theoretical and applied genetics. 2021 Aug;134(8):2495-2515. Epub 2021 May 2. doi: 10.1007/s00122-021-03838-7
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title = "Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs",
abstract = "Key message: Rose has 19 MLO genes. Of these, RhMLO1 and RhMLO2 were shown to be required for powdery mildew infection, which suggests their potential as susceptibility targets towards disease resistance. Abstract: Powdery mildew, caused by Podosphaera pannosa, is one of the most serious and widespread fungal diseases for roses, especially in greenhouse-grown cut roses. It has been shown that certain MLO genes are involved in powdery mildew susceptibility and that loss of function in these genes in various crops leads to broad-spectrum, long-lasting resistance against this fungal disease. For this reason, these MLO genes are called susceptibility genes. We carried out a genome-wide identification of the MLO gene family in the Rosa chinensis genome, and screened for allelic variants among 22 accessions from seven different Rosa species using re-sequencing and transcriptome data. We identified 19 MLO genes in rose, of which four are candidate genes for functional homologs in clade V, which is the clade containing all dicot MLO susceptibility genes. We detected a total of 198 different allelic variants in the set of Rosa species and accessions, corresponding to 5–15 different alleles for each of the genes. Some diploid Rosa species shared alleles with tetraploid rose cultivars, consistent with the notion that diploid species have contributed to the formation of tetraploid roses. Among the four RhMLO genes in clade V, we demonstrated using expression study, virus-induced gene silencing as well as transient RNAi silencing that two of them, RhMLO1 and RhMLO2, are required for infection by P. pannosa and suggest their potential as susceptibility targets for powdery mildew resistance breeding in rose.",
author = "Peihong Fang and Paul Arens and Xintong Liu and Xin Zhang and Deepika Lakwani and Fabrice Foucher and J{\'e}r{\'e}my Clotault and Juliane Geike and Helgard Kaufmann and Thomas Debener and Yuling Bai and Zhao Zhang and Smulders, {Marinus J.M.}",
note = "Funding Information: This work was supported by the National Natural Science Foundation of China (Grants Number 31501791) to Zhao Zhang, by a China Scholarship Council fellowship to Peihong Fang, and by TKI T&U (TU18142). We acknowledge Ms. Zhiyi Yu and Ms. Shuyao Deng for technical assistance and plant care. ",
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TY - JOUR

T1 - Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs

AU - Fang, Peihong

AU - Arens, Paul

AU - Liu, Xintong

AU - Zhang, Xin

AU - Lakwani, Deepika

AU - Foucher, Fabrice

AU - Clotault, Jérémy

AU - Geike, Juliane

AU - Kaufmann, Helgard

AU - Debener, Thomas

AU - Bai, Yuling

AU - Zhang, Zhao

AU - Smulders, Marinus J.M.

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (Grants Number 31501791) to Zhao Zhang, by a China Scholarship Council fellowship to Peihong Fang, and by TKI T&U (TU18142). We acknowledge Ms. Zhiyi Yu and Ms. Shuyao Deng for technical assistance and plant care.

PY - 2021/8

Y1 - 2021/8

N2 - Key message: Rose has 19 MLO genes. Of these, RhMLO1 and RhMLO2 were shown to be required for powdery mildew infection, which suggests their potential as susceptibility targets towards disease resistance. Abstract: Powdery mildew, caused by Podosphaera pannosa, is one of the most serious and widespread fungal diseases for roses, especially in greenhouse-grown cut roses. It has been shown that certain MLO genes are involved in powdery mildew susceptibility and that loss of function in these genes in various crops leads to broad-spectrum, long-lasting resistance against this fungal disease. For this reason, these MLO genes are called susceptibility genes. We carried out a genome-wide identification of the MLO gene family in the Rosa chinensis genome, and screened for allelic variants among 22 accessions from seven different Rosa species using re-sequencing and transcriptome data. We identified 19 MLO genes in rose, of which four are candidate genes for functional homologs in clade V, which is the clade containing all dicot MLO susceptibility genes. We detected a total of 198 different allelic variants in the set of Rosa species and accessions, corresponding to 5–15 different alleles for each of the genes. Some diploid Rosa species shared alleles with tetraploid rose cultivars, consistent with the notion that diploid species have contributed to the formation of tetraploid roses. Among the four RhMLO genes in clade V, we demonstrated using expression study, virus-induced gene silencing as well as transient RNAi silencing that two of them, RhMLO1 and RhMLO2, are required for infection by P. pannosa and suggest their potential as susceptibility targets for powdery mildew resistance breeding in rose.

AB - Key message: Rose has 19 MLO genes. Of these, RhMLO1 and RhMLO2 were shown to be required for powdery mildew infection, which suggests their potential as susceptibility targets towards disease resistance. Abstract: Powdery mildew, caused by Podosphaera pannosa, is one of the most serious and widespread fungal diseases for roses, especially in greenhouse-grown cut roses. It has been shown that certain MLO genes are involved in powdery mildew susceptibility and that loss of function in these genes in various crops leads to broad-spectrum, long-lasting resistance against this fungal disease. For this reason, these MLO genes are called susceptibility genes. We carried out a genome-wide identification of the MLO gene family in the Rosa chinensis genome, and screened for allelic variants among 22 accessions from seven different Rosa species using re-sequencing and transcriptome data. We identified 19 MLO genes in rose, of which four are candidate genes for functional homologs in clade V, which is the clade containing all dicot MLO susceptibility genes. We detected a total of 198 different allelic variants in the set of Rosa species and accessions, corresponding to 5–15 different alleles for each of the genes. Some diploid Rosa species shared alleles with tetraploid rose cultivars, consistent with the notion that diploid species have contributed to the formation of tetraploid roses. Among the four RhMLO genes in clade V, we demonstrated using expression study, virus-induced gene silencing as well as transient RNAi silencing that two of them, RhMLO1 and RhMLO2, are required for infection by P. pannosa and suggest their potential as susceptibility targets for powdery mildew resistance breeding in rose.

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