Details
| Original language | English |
|---|---|
| Pages (from-to) | 60-70 |
| Number of pages | 11 |
| Journal | Journal of biotechnology |
| Volume | 187 |
| Publication status | Published - 12 Jul 2014 |
Abstract
Serial repitching of brewing yeast inoculates is an important economic factor in the brewing industry, as their propagation is time and resource intensive. Here, we investigated whether replicative aging and/or the population distribution status changed during serial repitching in three different breweries with the same brewing yeast strain but different abiotic backgrounds and repitching regimes with varying numbers of reuses. Next to bud scar numbers the DNA content of the Saccharomyces pastorianus HEBRU cells was analyzed. Gene expression patterns were investigated using low-density microarrays with genes for aging, stress, storage compound metabolism and cell cycle.Two breweries showed a stable rejuvenation rate during serial repitching. In a third brewery the fraction of virgin cells varied, which could be explained with differing wort aeration rates. Furthermore, the number of bud scars per cell and cell size correlated in all 3 breweries throughout all runs. Transcriptome analyses revealed that from the 6th run on, mainly for the cells positive gene expression could be seen, for example up-regulation of trehalose and glycogen metabolism genes. Additionally, the cells' settling in the cone was dependent on cell size, with the lowest and the uppermost cone layers showing the highest amount of dead cells.In general, cells do not progressively age during extended serial repitching.
Keywords
- Bud scar counting, DNA content, Replicative aging, Saccharomyces pastorianus HEBRU, Serial repitching
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of biotechnology, Vol. 187, 12.07.2014, p. 60-70.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Analysis of aging in lager brewing yeast during serial repitching
AU - Bühligen, Franziska
AU - Lindner, Patrick
AU - Fetzer, Ingo
AU - Stahl, Frank
AU - Scheper, Thomas
AU - Harms, Hauke
AU - Müller, Susann
N1 - Funding information: F. B. was supported by the Scholarship Program of the German Federal Environmental Foundation (Grant number: AZ 20007/918 ). The work was supported by the Deutsche Brauwissenschaftsförderung (Grant number: R 422 ). The support of the Helmholtz Centre for Environmental Research (UFZ, IP Renewable Energy) is gratefully acknowledged.
PY - 2014/7/12
Y1 - 2014/7/12
N2 - Serial repitching of brewing yeast inoculates is an important economic factor in the brewing industry, as their propagation is time and resource intensive. Here, we investigated whether replicative aging and/or the population distribution status changed during serial repitching in three different breweries with the same brewing yeast strain but different abiotic backgrounds and repitching regimes with varying numbers of reuses. Next to bud scar numbers the DNA content of the Saccharomyces pastorianus HEBRU cells was analyzed. Gene expression patterns were investigated using low-density microarrays with genes for aging, stress, storage compound metabolism and cell cycle.Two breweries showed a stable rejuvenation rate during serial repitching. In a third brewery the fraction of virgin cells varied, which could be explained with differing wort aeration rates. Furthermore, the number of bud scars per cell and cell size correlated in all 3 breweries throughout all runs. Transcriptome analyses revealed that from the 6th run on, mainly for the cells positive gene expression could be seen, for example up-regulation of trehalose and glycogen metabolism genes. Additionally, the cells' settling in the cone was dependent on cell size, with the lowest and the uppermost cone layers showing the highest amount of dead cells.In general, cells do not progressively age during extended serial repitching.
AB - Serial repitching of brewing yeast inoculates is an important economic factor in the brewing industry, as their propagation is time and resource intensive. Here, we investigated whether replicative aging and/or the population distribution status changed during serial repitching in three different breweries with the same brewing yeast strain but different abiotic backgrounds and repitching regimes with varying numbers of reuses. Next to bud scar numbers the DNA content of the Saccharomyces pastorianus HEBRU cells was analyzed. Gene expression patterns were investigated using low-density microarrays with genes for aging, stress, storage compound metabolism and cell cycle.Two breweries showed a stable rejuvenation rate during serial repitching. In a third brewery the fraction of virgin cells varied, which could be explained with differing wort aeration rates. Furthermore, the number of bud scars per cell and cell size correlated in all 3 breweries throughout all runs. Transcriptome analyses revealed that from the 6th run on, mainly for the cells positive gene expression could be seen, for example up-regulation of trehalose and glycogen metabolism genes. Additionally, the cells' settling in the cone was dependent on cell size, with the lowest and the uppermost cone layers showing the highest amount of dead cells.In general, cells do not progressively age during extended serial repitching.
KW - Bud scar counting
KW - DNA content
KW - Replicative aging
KW - Saccharomyces pastorianus HEBRU
KW - Serial repitching
UR - http://www.scopus.com/inward/record.url?scp=84905833850&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2014.07.002
DO - 10.1016/j.jbiotec.2014.07.002
M3 - Article
C2 - 25026460
AN - SCOPUS:84905833850
VL - 187
SP - 60
EP - 70
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
ER -