Details
| Original language | English |
|---|---|
| Journal | Plant Genome |
| Volume | 7 |
| Issue number | 3 |
| Publication status | Published - 1 Nov 2014 |
Abstract
In some plant genera that contain species with complex genomes, the level and type of ploidy are still unknown due to a lack of characterized reference species and contradictory results from genetic and cytogenetic studies. Herein, we present the analysis of the genome of garden dahlias using molecular markers; this species is one for which the genome ploidy has remained controversial. We generated simple-sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) data from two segregating populations of garden dahlias. The combined analysis of SSR marker segregation, the ratio of single-dose to multidose markers, the ratio of markers linked in coupling and repulsion, and map construction revealed a predominantly autooctoploid genome with a low degree of preferential pairing. This finding indicates that dahlias are segmental allooctoploids that originated from autotetraploid ancestral genomes. Our results demonstrate that marker analysis is a suitable method for ploidy analysis in nonmodel crops. Novel marker techniques, such as restriction site associated DNA, will make this analysis even more effective before whole genome sequencing can be realized for these crops.
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant Genome, Vol. 7, No. 3, 01.11.2014.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Analysis of a complex polyploid plant genome using molecular markers
T2 - Strong evidence for segmental allooctoploidy in garden dahlias
AU - Schie, Stephan
AU - Chaudhary, Rajiv
AU - Debener, Thomas
N1 - Publisher Copyright: © Crop Science Society of America.
PY - 2014/11/1
Y1 - 2014/11/1
N2 - In some plant genera that contain species with complex genomes, the level and type of ploidy are still unknown due to a lack of characterized reference species and contradictory results from genetic and cytogenetic studies. Herein, we present the analysis of the genome of garden dahlias using molecular markers; this species is one for which the genome ploidy has remained controversial. We generated simple-sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) data from two segregating populations of garden dahlias. The combined analysis of SSR marker segregation, the ratio of single-dose to multidose markers, the ratio of markers linked in coupling and repulsion, and map construction revealed a predominantly autooctoploid genome with a low degree of preferential pairing. This finding indicates that dahlias are segmental allooctoploids that originated from autotetraploid ancestral genomes. Our results demonstrate that marker analysis is a suitable method for ploidy analysis in nonmodel crops. Novel marker techniques, such as restriction site associated DNA, will make this analysis even more effective before whole genome sequencing can be realized for these crops.
AB - In some plant genera that contain species with complex genomes, the level and type of ploidy are still unknown due to a lack of characterized reference species and contradictory results from genetic and cytogenetic studies. Herein, we present the analysis of the genome of garden dahlias using molecular markers; this species is one for which the genome ploidy has remained controversial. We generated simple-sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) data from two segregating populations of garden dahlias. The combined analysis of SSR marker segregation, the ratio of single-dose to multidose markers, the ratio of markers linked in coupling and repulsion, and map construction revealed a predominantly autooctoploid genome with a low degree of preferential pairing. This finding indicates that dahlias are segmental allooctoploids that originated from autotetraploid ancestral genomes. Our results demonstrate that marker analysis is a suitable method for ploidy analysis in nonmodel crops. Novel marker techniques, such as restriction site associated DNA, will make this analysis even more effective before whole genome sequencing can be realized for these crops.
UR - http://www.scopus.com/inward/record.url?scp=84908426074&partnerID=8YFLogxK
U2 - 10.3835/plantgenome2014.01.0002
DO - 10.3835/plantgenome2014.01.0002
M3 - Article
AN - SCOPUS:84908426074
VL - 7
JO - Plant Genome
JF - Plant Genome
SN - 1940-3372
IS - 3
ER -