Analysis and design of stimulus response curves of E. coli

Research output: Contribution to journal › Article › Research › peer review

Authors

  • Andreas Kremling
  • Anna Goehler
  • Knut Jahreis
  • Markus Nees
  • Benedikt Auerbach
  • Wolfgang Schmidt-Heck
  • Öznur Kökpinar
  • Robert Geffers
  • Ursula Rinas
  • Katja Bettenbrock

Research Organisations

External Research Organisations

  • Technical University of Munich (TUM)
  • Osnabrück University
  • Max Planck Institute for Dynamics of Complex Technical Systems
  • Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute (HKI)
  • Helmholtz Centre for Infection Research (HZI)
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Details

Original languageEnglish
Pages (from-to)844-871
Number of pages28
JournalMetabolites
Volume2
Issue number4
Publication statusPublished - 12 Nov 2012

Abstract

Metabolism and signalling are tightly coupled in bacteria. Combining several theoretical approaches, a core model is presented that describes transcriptional and allosteric control of glycolysis in Escherichia coli. Experimental data based on microarrays, signalling components and extracellular metabolites are used to estimate kinetic parameters. A newly designed strain was used that adjusts the incoming glucose flux into the system and allows a kinetic analysis. Based on the results, prediction for intracelluar metabolite concentrations over a broad range of the growth rate could be performed and compared with data from literature.

Keywords

    Carbohydrate uptake, Escherichia coli, Feedforward control, Gene regulatory network, Network component analysis, Parameter estimation

ASJC Scopus subject areas

Sustainable Development Goals

Cite this

Analysis and design of stimulus response curves of E. coli. / Kremling, Andreas; Goehler, Anna; Jahreis, Knut et al.
In: Metabolites, Vol. 2, No. 4, 12.11.2012, p. 844-871.

Research output: Contribution to journal › Article › Research › peer review

Kremling, A, Goehler, A, Jahreis, K, Nees, M, Auerbach, B, Schmidt-Heck, W, Kökpinar, Ö, Geffers, R, Rinas, U & Bettenbrock, K 2012, 'Analysis and design of stimulus response curves of E. coli', Metabolites, vol. 2, no. 4, pp. 844-871. https://doi.org/10.3390/metabo2040844, https://doi.org/10.15488/1554
Kremling, A., Goehler, A., Jahreis, K., Nees, M., Auerbach, B., Schmidt-Heck, W., Kökpinar, Ö., Geffers, R., Rinas, U., & Bettenbrock, K. (2012). Analysis and design of stimulus response curves of E. coli. Metabolites, 2(4), 844-871. https://doi.org/10.3390/metabo2040844, https://doi.org/10.15488/1554
Kremling A, Goehler A, Jahreis K, Nees M, Auerbach B, Schmidt-Heck W et al. Analysis and design of stimulus response curves of E. coli. Metabolites. 2012 Nov 12;2(4):844-871. doi: 10.3390/metabo2040844, https://doi.org/10.15488/1554
Kremling, Andreas ; Goehler, Anna ; Jahreis, Knut et al. / Analysis and design of stimulus response curves of E. coli. In: Metabolites. 2012 ; Vol. 2, No. 4. pp. 844-871.
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