Details
| Original language | English |
|---|---|
| Pages (from-to) | 143-154 |
| Number of pages | 12 |
| Journal | Journal of biotechnology |
| Volume | 26 |
| Issue number | 2-3 |
| Publication status | Published - Nov 1992 |
Abstract
The extraction of Penicillin G (PG) from the filtered cultivation medium of Penicillium chrysogenum and its conversion into 6-amino penicillanic acid (6-APA) and phenyl acetic acid (PhA) at pH 8 was performed in a 10 l Kühni extractor during the production by means of penicillin-G-amidase immobilized in a liquid membrane carrier system (LM). 6-APA was enriched in LM, and the PhA returned to the cultivation medium. After electrocoalescence of LM, the 6-APA was converted into ampicillin with the same enzyme at pH 6, while the liquid membrane phase and enzyme were recycled and reused.
Keywords
- 6-APA, Ampicillin, Biotransformation, Enzyme liquid membrane reactor, Penicillin G, Penicillin G-amidase
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of biotechnology, Vol. 26, No. 2-3, 11.1992, p. 143-154.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - An integrated process for the production and biotransformation of penicillin
AU - Barenschee, T.
AU - Scheper, T.
AU - Schügerl, K.
N1 - Funding information: The authors acknowledge Hoechst Co., H6chst, and the Swedish Scientific Foundation for the financial support, and thank Prof. Setterwall as well as Dr. A. Hasler, Dr. S. Hotop, Dr. A. Neumann, Dr. C. Nowottny, and Dr. D. Tsikas for their cooperation.
PY - 1992/11
Y1 - 1992/11
N2 - The extraction of Penicillin G (PG) from the filtered cultivation medium of Penicillium chrysogenum and its conversion into 6-amino penicillanic acid (6-APA) and phenyl acetic acid (PhA) at pH 8 was performed in a 10 l Kühni extractor during the production by means of penicillin-G-amidase immobilized in a liquid membrane carrier system (LM). 6-APA was enriched in LM, and the PhA returned to the cultivation medium. After electrocoalescence of LM, the 6-APA was converted into ampicillin with the same enzyme at pH 6, while the liquid membrane phase and enzyme were recycled and reused.
AB - The extraction of Penicillin G (PG) from the filtered cultivation medium of Penicillium chrysogenum and its conversion into 6-amino penicillanic acid (6-APA) and phenyl acetic acid (PhA) at pH 8 was performed in a 10 l Kühni extractor during the production by means of penicillin-G-amidase immobilized in a liquid membrane carrier system (LM). 6-APA was enriched in LM, and the PhA returned to the cultivation medium. After electrocoalescence of LM, the 6-APA was converted into ampicillin with the same enzyme at pH 6, while the liquid membrane phase and enzyme were recycled and reused.
KW - 6-APA
KW - Ampicillin
KW - Biotransformation
KW - Enzyme liquid membrane reactor
KW - Penicillin G
KW - Penicillin G-amidase
UR - http://www.scopus.com/inward/record.url?scp=0026716790&partnerID=8YFLogxK
U2 - 10.1016/0168-1656(92)90002-Q
DO - 10.1016/0168-1656(92)90002-Q
M3 - Article
C2 - 1369419
AN - SCOPUS:0026716790
VL - 26
SP - 143
EP - 154
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 2-3
ER -