Details
| Original language | English |
|---|---|
| Pages (from-to) | 157-160 |
| Number of pages | 4 |
| Journal | Analytical biochemistry |
| Volume | 176 |
| Issue number | 1 |
| Publication status | Published - Jan 1989 |
| Externally published | Yes |
Abstract
A simple and inexpensive system for equilibrium dialysis-based binding assays has been developed employing 1.5-ml microtubes. The main advantages are small volume of test buffers, no time-consuming assembly of the test vials, and inexpensive test cells. A comparison of the results derived from this equilibrium dialysis system with those of polyethylenimine-filtration assay and those of a commercially available equilibrium dialysis test system (Diachema from Dianorm) shows good agreement.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
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In: Analytical biochemistry, Vol. 176, No. 1, 01.1989, p. 157-160.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - An Inexpensive Small Volume Equilibrium Dialysis System for Protein-Ligand Binding Assays
AU - Reinard, Thomas
AU - Jacobsen, Hans Jörg
PY - 1989/1
Y1 - 1989/1
N2 - A simple and inexpensive system for equilibrium dialysis-based binding assays has been developed employing 1.5-ml microtubes. The main advantages are small volume of test buffers, no time-consuming assembly of the test vials, and inexpensive test cells. A comparison of the results derived from this equilibrium dialysis system with those of polyethylenimine-filtration assay and those of a commercially available equilibrium dialysis test system (Diachema from Dianorm) shows good agreement.
AB - A simple and inexpensive system for equilibrium dialysis-based binding assays has been developed employing 1.5-ml microtubes. The main advantages are small volume of test buffers, no time-consuming assembly of the test vials, and inexpensive test cells. A comparison of the results derived from this equilibrium dialysis system with those of polyethylenimine-filtration assay and those of a commercially available equilibrium dialysis test system (Diachema from Dianorm) shows good agreement.
UR - http://www.scopus.com/inward/record.url?scp=0024551980&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(89)90286-8
DO - 10.1016/0003-2697(89)90286-8
M3 - Article
C2 - 2712285
AN - SCOPUS:0024551980
VL - 176
SP - 157
EP - 160
JO - Analytical biochemistry
JF - Analytical biochemistry
SN - 0003-2697
IS - 1
ER -