Details
| Original language | English |
|---|---|
| Pages (from-to) | 177-186 |
| Number of pages | 10 |
| Journal | Journal of the Mechanical Behavior of Biomedical Materials |
| Volume | 60 |
| Publication status | Published - 11 Jan 2016 |
Abstract
The relationship between cell viability and adhesion behavior, and micro-deformation mechanisms was investigated on austenitic 316L stainless steel samples, which were subjected to different amounts of plastic strains (5%, 15%, 25%, 35% and 60%) to promote a variety in the slip and twin activities in the microstructure. Confocal laser scanning microscopy (CLSM) and field emission scanning electron microscopy (FESEM) revealed that cells most favored the samples with the largest plastic deformation, such that they spread more and formed significant filopodial extensions. Specifically, brain tumor cells seeded on the 35% deformed samples exhibited the best adhesion performance, where a significant slip activity was prevalent, accompanied by considerable slip-twin interactions. Furthermore, maximum viability was exhibited by the cells seeded on the 60% deformed samples, which were particularly designed in a specific geometry that could endure greater strain values. Overall, the current findings open a new venue for the production of metallic implants with enhanced biocompatibility, such that the adhesion and viability of the cells surrounding an implant can be optimized by tailoring the surface relief of the material, which is dictated by the micro-deformation mechanism activities facilitated by plastic deformation imposed by machining.
Keywords
- Biocompatibility, Cell adhesion, Implant material, Micro-deformation mechanism, Plastic deformation, Viability
ASJC Scopus subject areas
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Biomedical Engineering
- Engineering(all)
- Mechanics of Materials
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In: Journal of the Mechanical Behavior of Biomedical Materials, Vol. 60, 11.01.2016, p. 177-186.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - An exploration of plastic deformation dependence of cell viability and adhesion in metallic implant materials
AU - Uzer, B.
AU - Toker, S. M.
AU - Cingoz, A.
AU - Bagci-Onder, T.
AU - Gerstein, G.
AU - Maier, H. J.
AU - Canadinc, D.
N1 - Funding information: D. Canadinc acknowledges the financial support by the Turkish Academy of Sciences (TÜBA) within the Outstanding Young Scientist Program (GEBl.P). B. Uzer acknowledges the financial support by the Scientific and Technological Research Council of Turkey (TÜBl.TAK) within the National Graduate Student Fellowship Program 2211.
PY - 2016/1/11
Y1 - 2016/1/11
N2 - The relationship between cell viability and adhesion behavior, and micro-deformation mechanisms was investigated on austenitic 316L stainless steel samples, which were subjected to different amounts of plastic strains (5%, 15%, 25%, 35% and 60%) to promote a variety in the slip and twin activities in the microstructure. Confocal laser scanning microscopy (CLSM) and field emission scanning electron microscopy (FESEM) revealed that cells most favored the samples with the largest plastic deformation, such that they spread more and formed significant filopodial extensions. Specifically, brain tumor cells seeded on the 35% deformed samples exhibited the best adhesion performance, where a significant slip activity was prevalent, accompanied by considerable slip-twin interactions. Furthermore, maximum viability was exhibited by the cells seeded on the 60% deformed samples, which were particularly designed in a specific geometry that could endure greater strain values. Overall, the current findings open a new venue for the production of metallic implants with enhanced biocompatibility, such that the adhesion and viability of the cells surrounding an implant can be optimized by tailoring the surface relief of the material, which is dictated by the micro-deformation mechanism activities facilitated by plastic deformation imposed by machining.
AB - The relationship between cell viability and adhesion behavior, and micro-deformation mechanisms was investigated on austenitic 316L stainless steel samples, which were subjected to different amounts of plastic strains (5%, 15%, 25%, 35% and 60%) to promote a variety in the slip and twin activities in the microstructure. Confocal laser scanning microscopy (CLSM) and field emission scanning electron microscopy (FESEM) revealed that cells most favored the samples with the largest plastic deformation, such that they spread more and formed significant filopodial extensions. Specifically, brain tumor cells seeded on the 35% deformed samples exhibited the best adhesion performance, where a significant slip activity was prevalent, accompanied by considerable slip-twin interactions. Furthermore, maximum viability was exhibited by the cells seeded on the 60% deformed samples, which were particularly designed in a specific geometry that could endure greater strain values. Overall, the current findings open a new venue for the production of metallic implants with enhanced biocompatibility, such that the adhesion and viability of the cells surrounding an implant can be optimized by tailoring the surface relief of the material, which is dictated by the micro-deformation mechanism activities facilitated by plastic deformation imposed by machining.
KW - Biocompatibility
KW - Cell adhesion
KW - Implant material
KW - Micro-deformation mechanism
KW - Plastic deformation
KW - Viability
UR - http://www.scopus.com/inward/record.url?scp=84955118639&partnerID=8YFLogxK
U2 - 10.1016/j.jmbbm.2016.01.001
DO - 10.1016/j.jmbbm.2016.01.001
M3 - Article
C2 - 26807771
AN - SCOPUS:84955118639
VL - 60
SP - 177
EP - 186
JO - Journal of the Mechanical Behavior of Biomedical Materials
JF - Journal of the Mechanical Behavior of Biomedical Materials
SN - 1751-6161
ER -