Details
| Original language | English |
|---|---|
| Article number | 66 |
| Number of pages | 15 |
| Journal | Bioengineering |
| Volume | 9 |
| Issue number | 2 |
| Publication status | Published - 6 Feb 2022 |
| Externally published | Yes |
Abstract
Mesenchymal stem cells (MSCs) are primary candidates in tissue engineering and stem cell therapies due to their intriguing regenerative and immunomodulatory potential. Their ability to self-assemble into three-dimensional (3D) aggregates further improves some of their therapeutic properties, e.g., differentiation potential, secretion of cytokines, and homing capacity after administration. However, high hydrodynamic shear forces and the resulting mechanical stresses within commercially available dynamic cultivation systems can decrease their regenerative properties. Cells embedded within a polymer matrix, however, lack cell-to-cell interactions found in their physiological environment. Here, we present a “semi scaffold-free” approach to protect the cells from high shear forces by a physical barrier, but still allow formation of a 3D structure with in vivo-like cell-to-cell contacts. We highlight a relatively simple method to create core–shell capsules by inverse gelation. The capsules consist of an outer barrier made from sodium alginate, which allows for nutrient and waste diffusion and an inner compartment for direct cell-cell interactions. Next to capsule characterization, a harvesting procedure was established and viability and proliferation of human adipose-derived MSCs were investigated. In the future, this encapsulation and cultivation tech-nique might be used for MSC-expansion in scalable dynamic bioreactor systems, facilitating down-stream procedures, such as cell harvest and differentiation into mature tissue grafts.
Keywords
- 3D cell cultivation technologies, Alginate, Cell expansion, Core–shell capsule, Mesenchymal stem cells
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
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In: Bioengineering, Vol. 9, No. 2, 66, 06.02.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Alginate Core–Shell Capsules for 3D Cultivation of Adipose-Derived Mesenchymal Stem Cells
AU - Nebel, Sabrina
AU - Lux, Manuel
AU - Kuth, Sonja
AU - Bider, Faina
AU - Dietrich, Wolf
AU - Egger, Dominik
AU - Boccaccini, Aldo R.
AU - Kasper, Cornelia
N1 - Funding Information: Parts of this project were funded by the Doctoral School BioMatInt. Acknowledgments: We thank Markus Hofinger, Thomas Dalik, the Department of Food Science and Technology, and the Department of Chemistry for providing thickening agents for the initial testing; Christian Obruca (University Hospital Tulln) for enabling collection of human tissue; as well as Erik Reimhult and Eva-Kathrin Ehmoser from the Department of Nanobiotechnology for their advice and expertise. Further, we thank Monika Debreczeny for her help with the microscope ex-periments. The CLSM was kindly provided by the BOKU Core Facilities Multiscale Imaging. Funding Information: Funding: Parts of this project were funded by the Doctoral School BioMatInt.
PY - 2022/2/6
Y1 - 2022/2/6
N2 - Mesenchymal stem cells (MSCs) are primary candidates in tissue engineering and stem cell therapies due to their intriguing regenerative and immunomodulatory potential. Their ability to self-assemble into three-dimensional (3D) aggregates further improves some of their therapeutic properties, e.g., differentiation potential, secretion of cytokines, and homing capacity after administration. However, high hydrodynamic shear forces and the resulting mechanical stresses within commercially available dynamic cultivation systems can decrease their regenerative properties. Cells embedded within a polymer matrix, however, lack cell-to-cell interactions found in their physiological environment. Here, we present a “semi scaffold-free” approach to protect the cells from high shear forces by a physical barrier, but still allow formation of a 3D structure with in vivo-like cell-to-cell contacts. We highlight a relatively simple method to create core–shell capsules by inverse gelation. The capsules consist of an outer barrier made from sodium alginate, which allows for nutrient and waste diffusion and an inner compartment for direct cell-cell interactions. Next to capsule characterization, a harvesting procedure was established and viability and proliferation of human adipose-derived MSCs were investigated. In the future, this encapsulation and cultivation tech-nique might be used for MSC-expansion in scalable dynamic bioreactor systems, facilitating down-stream procedures, such as cell harvest and differentiation into mature tissue grafts.
AB - Mesenchymal stem cells (MSCs) are primary candidates in tissue engineering and stem cell therapies due to their intriguing regenerative and immunomodulatory potential. Their ability to self-assemble into three-dimensional (3D) aggregates further improves some of their therapeutic properties, e.g., differentiation potential, secretion of cytokines, and homing capacity after administration. However, high hydrodynamic shear forces and the resulting mechanical stresses within commercially available dynamic cultivation systems can decrease their regenerative properties. Cells embedded within a polymer matrix, however, lack cell-to-cell interactions found in their physiological environment. Here, we present a “semi scaffold-free” approach to protect the cells from high shear forces by a physical barrier, but still allow formation of a 3D structure with in vivo-like cell-to-cell contacts. We highlight a relatively simple method to create core–shell capsules by inverse gelation. The capsules consist of an outer barrier made from sodium alginate, which allows for nutrient and waste diffusion and an inner compartment for direct cell-cell interactions. Next to capsule characterization, a harvesting procedure was established and viability and proliferation of human adipose-derived MSCs were investigated. In the future, this encapsulation and cultivation tech-nique might be used for MSC-expansion in scalable dynamic bioreactor systems, facilitating down-stream procedures, such as cell harvest and differentiation into mature tissue grafts.
KW - 3D cell cultivation technologies
KW - Alginate
KW - Cell expansion
KW - Core–shell capsule
KW - Mesenchymal stem cells
UR - http://www.scopus.com/inward/record.url?scp=85124240196&partnerID=8YFLogxK
U2 - 10.3390/bioengineering9020066
DO - 10.3390/bioengineering9020066
M3 - Article
AN - SCOPUS:85124240196
VL - 9
JO - Bioengineering
JF - Bioengineering
IS - 2
M1 - 66
ER -