Details
| Original language | English |
|---|---|
| Pages (from-to) | 896-906 |
| Number of pages | 11 |
| Journal | Biotechnology progress |
| Volume | 26 |
| Issue number | 3 |
| Publication status | Published - 13 Jan 2010 |
Abstract
A highly stable and sensitive amperometric alcohol biosensor was developed by immobilizing alcohol oxidase (AOX) through Polyamidoamine (PAMAM) dendrimers on a cysteamine-modified gold electrode surface. Ethanol determination is based on the consumption of dissolved oxygen content due to the enzymatic reaction. The decrease in oxygen level was monitored at -0.7 V vs. Ag/AgCl and correlated with ethanol concentration. Optimization of variables affecting the system was performed. The optimized ethanol biosensor showed a wide linearity from 0.025 to 1.0 mM with 100 s response time and detection limit of (LOD) 0.016 mM. In the characterization studies, besides linearity some parameters such as operational and storage stability, reproducibility, repeatability, and substrate specificity were studied in detail. Stability studies showed a good preservation of the bioanalytical properties of the sensor, 67% of its initial sensitivity was kept after 1 month storage at 4°C. The analytical characteristics of the system were also evaluated for alcohol determination in flow injection analysis (FIA) mode. Finally, proposed biosensor was applied for ethanol analysis in various alcoholic beverage as well as offline monitoring of alcohol production through the yeast cultivation.
Keywords
- Alcohol oxidase, Dendrimer, Electrochemical enzyme biosensor, Self-assembled monolayers
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
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In: Biotechnology progress, Vol. 26, No. 3, 13.01.2010, p. 896-906.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Alcohol Biosensing by Polyamidoamine (PAMAM)/Cysteamine/AlcoholOxidase-Modified Gold Electrode
AU - Akin, Mehriban
AU - Yuksel, Merve
AU - Geyik, Caner
AU - Odaci, Dilek
AU - Bluma, Arne
AU - Höpfner, Tim
AU - Beutel, Sascha
AU - Scheper, Thomas
AU - Timur, Suna
PY - 2010/1/13
Y1 - 2010/1/13
N2 - A highly stable and sensitive amperometric alcohol biosensor was developed by immobilizing alcohol oxidase (AOX) through Polyamidoamine (PAMAM) dendrimers on a cysteamine-modified gold electrode surface. Ethanol determination is based on the consumption of dissolved oxygen content due to the enzymatic reaction. The decrease in oxygen level was monitored at -0.7 V vs. Ag/AgCl and correlated with ethanol concentration. Optimization of variables affecting the system was performed. The optimized ethanol biosensor showed a wide linearity from 0.025 to 1.0 mM with 100 s response time and detection limit of (LOD) 0.016 mM. In the characterization studies, besides linearity some parameters such as operational and storage stability, reproducibility, repeatability, and substrate specificity were studied in detail. Stability studies showed a good preservation of the bioanalytical properties of the sensor, 67% of its initial sensitivity was kept after 1 month storage at 4°C. The analytical characteristics of the system were also evaluated for alcohol determination in flow injection analysis (FIA) mode. Finally, proposed biosensor was applied for ethanol analysis in various alcoholic beverage as well as offline monitoring of alcohol production through the yeast cultivation.
AB - A highly stable and sensitive amperometric alcohol biosensor was developed by immobilizing alcohol oxidase (AOX) through Polyamidoamine (PAMAM) dendrimers on a cysteamine-modified gold electrode surface. Ethanol determination is based on the consumption of dissolved oxygen content due to the enzymatic reaction. The decrease in oxygen level was monitored at -0.7 V vs. Ag/AgCl and correlated with ethanol concentration. Optimization of variables affecting the system was performed. The optimized ethanol biosensor showed a wide linearity from 0.025 to 1.0 mM with 100 s response time and detection limit of (LOD) 0.016 mM. In the characterization studies, besides linearity some parameters such as operational and storage stability, reproducibility, repeatability, and substrate specificity were studied in detail. Stability studies showed a good preservation of the bioanalytical properties of the sensor, 67% of its initial sensitivity was kept after 1 month storage at 4°C. The analytical characteristics of the system were also evaluated for alcohol determination in flow injection analysis (FIA) mode. Finally, proposed biosensor was applied for ethanol analysis in various alcoholic beverage as well as offline monitoring of alcohol production through the yeast cultivation.
KW - Alcohol oxidase
KW - Dendrimer
KW - Electrochemical enzyme biosensor
KW - Self-assembled monolayers
UR - http://www.scopus.com/inward/record.url?scp=77954732483&partnerID=8YFLogxK
U2 - 10.1002/btpr.372
DO - 10.1002/btpr.372
M3 - Article
C2 - 20073071
AN - SCOPUS:77954732483
VL - 26
SP - 896
EP - 906
JO - Biotechnology progress
JF - Biotechnology progress
SN - 8756-7938
IS - 3
ER -