Details
| Original language | English |
|---|---|
| Title of host publication | Acta Horticulturae |
| Pages | 149-154 |
| Number of pages | 6 |
| Publication status | Published - 13 Jun 2009 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 829 |
| ISSN (Print) | 0567-7572 |
Abstract
Ornamental orchids Oncidium and Odontoglossum are sensitivite to ethylene, and their senescence process is initiated very fast. Ethylene receptor mutant gene etr1-1 from Arabidopsis is known to prevent ethylene induced senescence, which can occur during transport or/and storage of plants. Therefore, the objective of our project was to transform Oncidium and Odontoglossum with etr1-1 under control of a flower-specific promoter to achieve a prolonged shelf life. Establishment of an efficient protocol for in vitro regeneration via somatic embryogenesis and Agrobacterium-mediated transformation was necessary for achievement of our aim. Starting from in vitro shoot cultures, we established a reliable protocol for the regeneration of protocorm-like-bodies (PLBs). By applying this regeneration protocol we tested different parameters for Agrobacterium-mediated gene transfer using PLBs in liquid culture. Using histochemical GUS assays transformation events were assessed after two, four, and six months. The molecular analyses of the putative transgenic plants are in progress.
Keywords
- Ethylene, In vitro culture, Orchids, PLBs, Senescence, Somatic embryogenesis
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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Acta Horticulturae. 2009. p. 149-154 (Acta Horticulturae; Vol. 829).
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - Agrobacterium tumefaciens-mediated transformation of Oncidium and Odontoglossum with the mutated ethylene receptor etr1-1
AU - Raffeiner, B.
AU - Winkelmann, T.
AU - Serek, M.
PY - 2009/6/13
Y1 - 2009/6/13
N2 - Ornamental orchids Oncidium and Odontoglossum are sensitivite to ethylene, and their senescence process is initiated very fast. Ethylene receptor mutant gene etr1-1 from Arabidopsis is known to prevent ethylene induced senescence, which can occur during transport or/and storage of plants. Therefore, the objective of our project was to transform Oncidium and Odontoglossum with etr1-1 under control of a flower-specific promoter to achieve a prolonged shelf life. Establishment of an efficient protocol for in vitro regeneration via somatic embryogenesis and Agrobacterium-mediated transformation was necessary for achievement of our aim. Starting from in vitro shoot cultures, we established a reliable protocol for the regeneration of protocorm-like-bodies (PLBs). By applying this regeneration protocol we tested different parameters for Agrobacterium-mediated gene transfer using PLBs in liquid culture. Using histochemical GUS assays transformation events were assessed after two, four, and six months. The molecular analyses of the putative transgenic plants are in progress.
AB - Ornamental orchids Oncidium and Odontoglossum are sensitivite to ethylene, and their senescence process is initiated very fast. Ethylene receptor mutant gene etr1-1 from Arabidopsis is known to prevent ethylene induced senescence, which can occur during transport or/and storage of plants. Therefore, the objective of our project was to transform Oncidium and Odontoglossum with etr1-1 under control of a flower-specific promoter to achieve a prolonged shelf life. Establishment of an efficient protocol for in vitro regeneration via somatic embryogenesis and Agrobacterium-mediated transformation was necessary for achievement of our aim. Starting from in vitro shoot cultures, we established a reliable protocol for the regeneration of protocorm-like-bodies (PLBs). By applying this regeneration protocol we tested different parameters for Agrobacterium-mediated gene transfer using PLBs in liquid culture. Using histochemical GUS assays transformation events were assessed after two, four, and six months. The molecular analyses of the putative transgenic plants are in progress.
KW - Ethylene
KW - In vitro culture
KW - Orchids
KW - PLBs
KW - Senescence
KW - Somatic embryogenesis
UR - http://www.scopus.com/inward/record.url?scp=74849127636&partnerID=8YFLogxK
M3 - Contribution to book/anthology
AN - SCOPUS:74849127636
SN - 9789066055223
T3 - Acta Horticulturae
SP - 149
EP - 154
BT - Acta Horticulturae
ER -