Details
| Original language | English |
|---|---|
| Pages (from-to) | 71-92 |
| Number of pages | 22 |
| Journal | Annual Review of Heat Transfer |
| Volume | 27 |
| Publication status | Published - 2024 |
Abstract
Cryopreservation is essential for the long-term storage of biological materials at sub-zero temperatures in regenerative medicine, research, and clinical applications. It refers to the process of preserving cells, tissues, and organs at sub-zero temperatures to maintain their viability and functionality. However, cryopreservation faces significant hurdles, particularly in the preservation of sensitive biological materials such as reproductive organs. This review highlights the critical role of cryogenic cooling instrumentation in managing heat transfer at each stage of cryopreservation: cooling, storage and transport, and thawing. Effective heat transfer is critical during the cooling process. Advanced cryogenic cooling instrumentation, such as controlled rate freezers and vitrification devices, is essential to optimize heat removal, thereby minimizing ice formation and cell damage. These instruments allow precise control of cooling rates, which is critical for protecting sensitive biological materials. In addition, recent advances in thawing technologies, such as nano warming and high-intensity focused ultrasound (HIFU), highlight the evolving capabilities of cryogenic instruments. These technologies enable rapid and uniform warming, which is critical for maintaining the structural integrity and functionality of cells. In addition, maintaining stable ultra-low temperatures during storage is essential to prevent molecular movement that could cause damage. This review discusses the role of cryogenic freezers in ensuring consistent storage conditions. In addition, innovations in cryogenic transport technologies ensure that samples are kept at controlled temperatures to prevent thermal degradation during transport. The article concludes with the continued need for advances in cryogenic cooling instrumentation within cryopreservation. Future improvements will likely involve the integration of more biophysical principles to develop sophisticated technologies that improve heat-transfer efficiency. These advancements will expand the practical applications and effectiveness of cryopreservation in the biomedical field.
Keywords
- controlled freezing, cooling rate, cryoprotective agents, ice seeding, thawing
ASJC Scopus subject areas
- Physics and Astronomy(all)
- Condensed Matter Physics
- Energy(all)
- Energy Engineering and Power Technology
- Engineering(all)
- Mechanical Engineering
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In: Annual Review of Heat Transfer, Vol. 27, 2024, p. 71-92.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Advancements in Cryogenic Cooling and Warming Instrumentation for Cryopreservation in Milliliter and Sub-Milliliter Scale
AU - Deeb, Tarek
AU - Leal-Marin, Sara
AU - Hubenia, Oleksandra
AU - Brunotte, Ricarda
AU - Glasmacher, Birgit
N1 - Publisher Copyright: © 2024 by Begell House, Inc.
PY - 2024
Y1 - 2024
N2 - Cryopreservation is essential for the long-term storage of biological materials at sub-zero temperatures in regenerative medicine, research, and clinical applications. It refers to the process of preserving cells, tissues, and organs at sub-zero temperatures to maintain their viability and functionality. However, cryopreservation faces significant hurdles, particularly in the preservation of sensitive biological materials such as reproductive organs. This review highlights the critical role of cryogenic cooling instrumentation in managing heat transfer at each stage of cryopreservation: cooling, storage and transport, and thawing. Effective heat transfer is critical during the cooling process. Advanced cryogenic cooling instrumentation, such as controlled rate freezers and vitrification devices, is essential to optimize heat removal, thereby minimizing ice formation and cell damage. These instruments allow precise control of cooling rates, which is critical for protecting sensitive biological materials. In addition, recent advances in thawing technologies, such as nano warming and high-intensity focused ultrasound (HIFU), highlight the evolving capabilities of cryogenic instruments. These technologies enable rapid and uniform warming, which is critical for maintaining the structural integrity and functionality of cells. In addition, maintaining stable ultra-low temperatures during storage is essential to prevent molecular movement that could cause damage. This review discusses the role of cryogenic freezers in ensuring consistent storage conditions. In addition, innovations in cryogenic transport technologies ensure that samples are kept at controlled temperatures to prevent thermal degradation during transport. The article concludes with the continued need for advances in cryogenic cooling instrumentation within cryopreservation. Future improvements will likely involve the integration of more biophysical principles to develop sophisticated technologies that improve heat-transfer efficiency. These advancements will expand the practical applications and effectiveness of cryopreservation in the biomedical field.
AB - Cryopreservation is essential for the long-term storage of biological materials at sub-zero temperatures in regenerative medicine, research, and clinical applications. It refers to the process of preserving cells, tissues, and organs at sub-zero temperatures to maintain their viability and functionality. However, cryopreservation faces significant hurdles, particularly in the preservation of sensitive biological materials such as reproductive organs. This review highlights the critical role of cryogenic cooling instrumentation in managing heat transfer at each stage of cryopreservation: cooling, storage and transport, and thawing. Effective heat transfer is critical during the cooling process. Advanced cryogenic cooling instrumentation, such as controlled rate freezers and vitrification devices, is essential to optimize heat removal, thereby minimizing ice formation and cell damage. These instruments allow precise control of cooling rates, which is critical for protecting sensitive biological materials. In addition, recent advances in thawing technologies, such as nano warming and high-intensity focused ultrasound (HIFU), highlight the evolving capabilities of cryogenic instruments. These technologies enable rapid and uniform warming, which is critical for maintaining the structural integrity and functionality of cells. In addition, maintaining stable ultra-low temperatures during storage is essential to prevent molecular movement that could cause damage. This review discusses the role of cryogenic freezers in ensuring consistent storage conditions. In addition, innovations in cryogenic transport technologies ensure that samples are kept at controlled temperatures to prevent thermal degradation during transport. The article concludes with the continued need for advances in cryogenic cooling instrumentation within cryopreservation. Future improvements will likely involve the integration of more biophysical principles to develop sophisticated technologies that improve heat-transfer efficiency. These advancements will expand the practical applications and effectiveness of cryopreservation in the biomedical field.
KW - controlled freezing
KW - cooling rate
KW - cryoprotective agents
KW - ice seeding
KW - thawing
UR - http://www.scopus.com/inward/record.url?scp=85215658466&partnerID=8YFLogxK
U2 - 10.1615/AnnualRevHeatTransfer.2024053933
DO - 10.1615/AnnualRevHeatTransfer.2024053933
M3 - Article
AN - SCOPUS:85215658466
VL - 27
SP - 71
EP - 92
JO - Annual Review of Heat Transfer
JF - Annual Review of Heat Transfer
SN - 1049-0787
ER -