Details
| Original language | English |
|---|---|
| Article number | 1436 |
| Number of pages | 13 |
| Journal | Pharmaceutics |
| Volume | 14 |
| Issue number | 7 |
| Publication status | Published - 8 Jul 2022 |
| Externally published | Yes |
Abstract
Skin equivalents and skin explants are widely used for dermal penetration studies in the pharmacological development of drugs. Environmental parameters, such as the incubation and culture conditions affect cellular responses and thus the relevance of the experimental outcome. However, available systems such as the Franz diffusion chamber, only measure in the receiving culture medium, rather than assessing the actual conditions for cells in the tissue. We developed a sampling design that combines open flow microperfusion (OFM) sampling technology for continuous concentration measurements directly in the tissue with microfluidic biosensors for online monitoring of culture parameters. We tested our design with real-time measurements of oxygen, glucose, lactate, and pH in full-thickness skin equivalent and skin explants. Furthermore, we compared dermal penetration for acyclovir, lidocaine, and diclofenac in skin equivalents and skin explants. We observed differences in oxygen, glucose, and drug concentrations in skin equivalents compared to the respective culture medium and to skin explants.
Keywords
- full-thickness skin equivalent, glucose, lactate, open flow microperfusion, oxygen, penetration tests, pH, pharmacokinetics, sensors, skin explants
ASJC Scopus subject areas
- Pharmacology, Toxicology and Pharmaceutics(all)
- Pharmaceutical Science
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In: Pharmaceutics, Vol. 14, No. 7, 1436, 08.07.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Advanced Online Monitoring of In Vitro Human 3D Full-Thickness Skin Equivalents
AU - Schaller-Ammann, Roland
AU - Kreß, Sebastian
AU - Feiel, Jürgen
AU - Schwagerle, Gerd
AU - Priedl, Joachim
AU - Birngruber, Thomas
AU - Kasper, Cornelia
AU - Egger, Dominik
N1 - Funding Information: Funding: This research was funded by Austrian Research Promotion Agency (FFG) grant number 859279.
PY - 2022/7/8
Y1 - 2022/7/8
N2 - Skin equivalents and skin explants are widely used for dermal penetration studies in the pharmacological development of drugs. Environmental parameters, such as the incubation and culture conditions affect cellular responses and thus the relevance of the experimental outcome. However, available systems such as the Franz diffusion chamber, only measure in the receiving culture medium, rather than assessing the actual conditions for cells in the tissue. We developed a sampling design that combines open flow microperfusion (OFM) sampling technology for continuous concentration measurements directly in the tissue with microfluidic biosensors for online monitoring of culture parameters. We tested our design with real-time measurements of oxygen, glucose, lactate, and pH in full-thickness skin equivalent and skin explants. Furthermore, we compared dermal penetration for acyclovir, lidocaine, and diclofenac in skin equivalents and skin explants. We observed differences in oxygen, glucose, and drug concentrations in skin equivalents compared to the respective culture medium and to skin explants.
AB - Skin equivalents and skin explants are widely used for dermal penetration studies in the pharmacological development of drugs. Environmental parameters, such as the incubation and culture conditions affect cellular responses and thus the relevance of the experimental outcome. However, available systems such as the Franz diffusion chamber, only measure in the receiving culture medium, rather than assessing the actual conditions for cells in the tissue. We developed a sampling design that combines open flow microperfusion (OFM) sampling technology for continuous concentration measurements directly in the tissue with microfluidic biosensors for online monitoring of culture parameters. We tested our design with real-time measurements of oxygen, glucose, lactate, and pH in full-thickness skin equivalent and skin explants. Furthermore, we compared dermal penetration for acyclovir, lidocaine, and diclofenac in skin equivalents and skin explants. We observed differences in oxygen, glucose, and drug concentrations in skin equivalents compared to the respective culture medium and to skin explants.
KW - full-thickness skin equivalent
KW - glucose
KW - lactate
KW - open flow microperfusion
KW - oxygen
KW - penetration tests
KW - pH
KW - pharmacokinetics
KW - sensors
KW - skin explants
UR - http://www.scopus.com/inward/record.url?scp=85134483762&partnerID=8YFLogxK
U2 - 10.3390/pharmaceutics14071436
DO - 10.3390/pharmaceutics14071436
M3 - Article
AN - SCOPUS:85134483762
VL - 14
JO - Pharmaceutics
JF - Pharmaceutics
IS - 7
M1 - 1436
ER -