Details
| Original language | English |
|---|---|
| Pages (from-to) | 847-861 |
| Number of pages | 15 |
| Journal | Bioprocess and Biosystems Engineering |
| Volume | 33 |
| Issue number | 7 |
| Publication status | Published - 26 Mar 2010 |
Abstract
The adsorption of proteins by a smectitic clay mineral was investigated. The clay used in this study is a mixture of montmorillonite and amorphous SiO2. Due to the high porosity the montmorillonite units are accessible for protein adsorption. The amorphous silica prevents the montmorillonite from swelling and allows column packing. Protein adsorption was performed at different pH under static conditions. Furthermore, static capacities were determined. The material reveals high adsorption capacities for proteins under static conditions (270-408 mg/g), whereby proteins are mainly adsorbed via electrostatic interactions. The Freundlich isotherm is suggested as an adsorption model. For desorption a pH shift was found to be most effective. Binding and elution of human serum albumin and ovalbumin were tested under dynamic conditions. Dynamic capacities of about 40 mg/g for ovalbumin at 764 cm/h were found. The clay mineral provides suitable properties for the application as cost-efficient, alternative separation material.
Keywords
- Clay mineral, Desorption, Montmorillonite, Protein adsorption, Separation, Smectite
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
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In: Bioprocess and Biosystems Engineering, Vol. 33, No. 7, 26.03.2010, p. 847-861.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Adsorption and separation of proteins by a smectitic clay mineral
AU - Ralla, Kathrin
AU - Sohling, Ulrich
AU - Riechers, Daniel
AU - Kasper, Cornelia
AU - Ruf, Friedrich
AU - Scheper, Thomas
PY - 2010/3/26
Y1 - 2010/3/26
N2 - The adsorption of proteins by a smectitic clay mineral was investigated. The clay used in this study is a mixture of montmorillonite and amorphous SiO2. Due to the high porosity the montmorillonite units are accessible for protein adsorption. The amorphous silica prevents the montmorillonite from swelling and allows column packing. Protein adsorption was performed at different pH under static conditions. Furthermore, static capacities were determined. The material reveals high adsorption capacities for proteins under static conditions (270-408 mg/g), whereby proteins are mainly adsorbed via electrostatic interactions. The Freundlich isotherm is suggested as an adsorption model. For desorption a pH shift was found to be most effective. Binding and elution of human serum albumin and ovalbumin were tested under dynamic conditions. Dynamic capacities of about 40 mg/g for ovalbumin at 764 cm/h were found. The clay mineral provides suitable properties for the application as cost-efficient, alternative separation material.
AB - The adsorption of proteins by a smectitic clay mineral was investigated. The clay used in this study is a mixture of montmorillonite and amorphous SiO2. Due to the high porosity the montmorillonite units are accessible for protein adsorption. The amorphous silica prevents the montmorillonite from swelling and allows column packing. Protein adsorption was performed at different pH under static conditions. Furthermore, static capacities were determined. The material reveals high adsorption capacities for proteins under static conditions (270-408 mg/g), whereby proteins are mainly adsorbed via electrostatic interactions. The Freundlich isotherm is suggested as an adsorption model. For desorption a pH shift was found to be most effective. Binding and elution of human serum albumin and ovalbumin were tested under dynamic conditions. Dynamic capacities of about 40 mg/g for ovalbumin at 764 cm/h were found. The clay mineral provides suitable properties for the application as cost-efficient, alternative separation material.
KW - Clay mineral
KW - Desorption
KW - Montmorillonite
KW - Protein adsorption
KW - Separation
KW - Smectite
UR - http://www.scopus.com/inward/record.url?scp=77956059569&partnerID=8YFLogxK
U2 - 10.1007/s00449-010-0408-8
DO - 10.1007/s00449-010-0408-8
M3 - Article
C2 - 20340034
AN - SCOPUS:77956059569
VL - 33
SP - 847
EP - 861
JO - Bioprocess and Biosystems Engineering
JF - Bioprocess and Biosystems Engineering
SN - 1615-7591
IS - 7
ER -