Details
| Original language | English |
|---|---|
| Article number | 015005 |
| Journal | BIOFABRICATION |
| Volume | 3 |
| Issue number | 1 |
| Publication status | Published - 1 Mar 2011 |
| Externally published | Yes |
Abstract
Laser-assisted bioprinting (LaBP) allows the realization of computer-generated 3D tissue grafts consisting of cells embedded in a hydrogel environment. In this study, human adipose-derived stem cells (hASCs) were printed in a free-scalable 3D grid pattern by means of LaBP. We demonstrate that neither the proliferation ability nor the differentiation behaviour of the stem cells was affected by the LaBP procedure. Furthermore, the 3D grafts were differentiated down the adipogenic lineage pathway for 10 days. We verify by quantitative assessments of adipogenic markers that the 3D grafts resemble cell lineages present in natural adipose tissue. Additionally, we provide the proof that even pre-differentiated hASCs could be utilized for the generation of 3D tissue grafts. These results indicate that the biofabrication of living grafts resembling their complex native origin is within reach.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Biomedical Engineering
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: BIOFABRICATION, Vol. 3, No. 1, 015005, 01.03.2011.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Adipogenic differentiation of laser-printed 3D tissue grafts consisting of human adipose-derived stem cells
AU - Gruene, M.
AU - Pflaum, M.
AU - Deiwick, A.
AU - Koch, L.
AU - Schlie, S.
AU - Unger, C.
AU - Wilhelmi, M.
AU - Haverich, A.
AU - Chichkov, B. N.
PY - 2011/3/1
Y1 - 2011/3/1
N2 - Laser-assisted bioprinting (LaBP) allows the realization of computer-generated 3D tissue grafts consisting of cells embedded in a hydrogel environment. In this study, human adipose-derived stem cells (hASCs) were printed in a free-scalable 3D grid pattern by means of LaBP. We demonstrate that neither the proliferation ability nor the differentiation behaviour of the stem cells was affected by the LaBP procedure. Furthermore, the 3D grafts were differentiated down the adipogenic lineage pathway for 10 days. We verify by quantitative assessments of adipogenic markers that the 3D grafts resemble cell lineages present in natural adipose tissue. Additionally, we provide the proof that even pre-differentiated hASCs could be utilized for the generation of 3D tissue grafts. These results indicate that the biofabrication of living grafts resembling their complex native origin is within reach.
AB - Laser-assisted bioprinting (LaBP) allows the realization of computer-generated 3D tissue grafts consisting of cells embedded in a hydrogel environment. In this study, human adipose-derived stem cells (hASCs) were printed in a free-scalable 3D grid pattern by means of LaBP. We demonstrate that neither the proliferation ability nor the differentiation behaviour of the stem cells was affected by the LaBP procedure. Furthermore, the 3D grafts were differentiated down the adipogenic lineage pathway for 10 days. We verify by quantitative assessments of adipogenic markers that the 3D grafts resemble cell lineages present in natural adipose tissue. Additionally, we provide the proof that even pre-differentiated hASCs could be utilized for the generation of 3D tissue grafts. These results indicate that the biofabrication of living grafts resembling their complex native origin is within reach.
UR - http://www.scopus.com/inward/record.url?scp=79958278727&partnerID=8YFLogxK
U2 - 10.1088/1758-5082/3/1/015005
DO - 10.1088/1758-5082/3/1/015005
M3 - Article
C2 - 21358040
AN - SCOPUS:79958278727
VL - 3
JO - BIOFABRICATION
JF - BIOFABRICATION
SN - 1758-5082
IS - 1
M1 - 015005
ER -