Details
| Original language | English |
|---|---|
| Article number | 567 |
| Journal | Bioengineering |
| Volume | 9 |
| Issue number | 10 |
| Publication status | Published - 17 Oct 2022 |
Abstract
Bacterial adhesion to dental implants is the onset for the development of pathological biofilms. Reliable characterization of this initial process is the basis towards the development of anti-biofilm strategies. In the present study, single-cell force spectroscopy (SCFS), by means of an atomic force microscope connected to a microfluidic pressure control system (FluidFM), was used to comparably measure adhesion forces of different oral bacteria within a similar experimental setup to the common implant material titanium. The bacteria selected belong to different ecological niches in oral biofilms: the commensal pioneers Streptococcus oralis and Actinomyces naeslundii; secondary colonizer Veillonella dispar; and the late colonizing pathogens Porphyromonas gingivalis as well as fimbriated and non-fimbriated Aggregatibacter actinomycetemcomitans. The results showed highest values for early colonizing pioneer species, strengthening the link between adhesion forces and bacteria’s role in oral biofilm development. Additionally, the correlation between biophysical cellular characteristics and SCFS results across species was analyzed. Here, distinct correlations between electrostatically driven maximum adhesion force, bacterial surface elasticity and surface charge as well as single-molecule attachment points, stretching capability and metabolic activity, could be identified. Therefore, this study provides a step towards the detailed understanding of oral bacteria initial adhesion and could support the development of infection-resistant implant materials in future.
Keywords
- atomic force microscopy, bacterial adhesion, cell respiration, cell surface, dental implant, single-cell spectroscopy
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
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In: Bioengineering, Vol. 9, No. 10, 567, 17.10.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Adhesion Forces of Oral Bacteria to Titanium and the Correlation with Biophysical Cellular Characteristics
AU - Doll-Nikutta, Katharina
AU - Winkel, Andreas
AU - Yang, Ines
AU - Grote, Anna Josefine
AU - Meier, Nils
AU - Habib, Mosaieb
AU - Menzel, Henning
AU - Behrens, Peter
AU - Stiesch, Meike
N1 - Funding Information: The work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under the Collaborative Research Center SFB/TRR-298-SIIRI—Project ID 426335750 and partially under Germany’s Excellence Strategy (DFG Cluster of Excellence) EXC 2177/1-Hearing4All—Project ID 390895286.
PY - 2022/10/17
Y1 - 2022/10/17
N2 - Bacterial adhesion to dental implants is the onset for the development of pathological biofilms. Reliable characterization of this initial process is the basis towards the development of anti-biofilm strategies. In the present study, single-cell force spectroscopy (SCFS), by means of an atomic force microscope connected to a microfluidic pressure control system (FluidFM), was used to comparably measure adhesion forces of different oral bacteria within a similar experimental setup to the common implant material titanium. The bacteria selected belong to different ecological niches in oral biofilms: the commensal pioneers Streptococcus oralis and Actinomyces naeslundii; secondary colonizer Veillonella dispar; and the late colonizing pathogens Porphyromonas gingivalis as well as fimbriated and non-fimbriated Aggregatibacter actinomycetemcomitans. The results showed highest values for early colonizing pioneer species, strengthening the link between adhesion forces and bacteria’s role in oral biofilm development. Additionally, the correlation between biophysical cellular characteristics and SCFS results across species was analyzed. Here, distinct correlations between electrostatically driven maximum adhesion force, bacterial surface elasticity and surface charge as well as single-molecule attachment points, stretching capability and metabolic activity, could be identified. Therefore, this study provides a step towards the detailed understanding of oral bacteria initial adhesion and could support the development of infection-resistant implant materials in future.
AB - Bacterial adhesion to dental implants is the onset for the development of pathological biofilms. Reliable characterization of this initial process is the basis towards the development of anti-biofilm strategies. In the present study, single-cell force spectroscopy (SCFS), by means of an atomic force microscope connected to a microfluidic pressure control system (FluidFM), was used to comparably measure adhesion forces of different oral bacteria within a similar experimental setup to the common implant material titanium. The bacteria selected belong to different ecological niches in oral biofilms: the commensal pioneers Streptococcus oralis and Actinomyces naeslundii; secondary colonizer Veillonella dispar; and the late colonizing pathogens Porphyromonas gingivalis as well as fimbriated and non-fimbriated Aggregatibacter actinomycetemcomitans. The results showed highest values for early colonizing pioneer species, strengthening the link between adhesion forces and bacteria’s role in oral biofilm development. Additionally, the correlation between biophysical cellular characteristics and SCFS results across species was analyzed. Here, distinct correlations between electrostatically driven maximum adhesion force, bacterial surface elasticity and surface charge as well as single-molecule attachment points, stretching capability and metabolic activity, could be identified. Therefore, this study provides a step towards the detailed understanding of oral bacteria initial adhesion and could support the development of infection-resistant implant materials in future.
KW - atomic force microscopy
KW - bacterial adhesion
KW - cell respiration
KW - cell surface
KW - dental implant
KW - single-cell spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=85140358409&partnerID=8YFLogxK
U2 - 10.3390/bioengineering9100567
DO - 10.3390/bioengineering9100567
M3 - Article
AN - SCOPUS:85140358409
VL - 9
JO - Bioengineering
JF - Bioengineering
IS - 10
M1 - 567
ER -