Details
| Original language | English |
|---|---|
| Article number | 950 |
| Journal | Nature Communications |
| Volume | 12 |
| Issue number | 1 |
| Publication status | Published - 11 Feb 2021 |
| Externally published | Yes |
Abstract
The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g., in point-of-care diagnostic settings, where costly equipment would be prohibitive. Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in NACHOS emit up to 461-fold (average of 89 ± 7-fold) brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia on the road.
ASJC Scopus subject areas
- Chemistry(all)
- General Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- General Biochemistry,Genetics and Molecular Biology
- Physics and Astronomy(all)
- General Physics and Astronomy
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In: Nature Communications, Vol. 12, No. 1, 950, 11.02.2021.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope
AU - Trofymchuk, Kateryna
AU - Glembockyte, Viktorija
AU - Grabenhorst, Lennart
AU - Steiner, Florian
AU - Vietz, Carolin
AU - Close, Cindy
AU - Pfeiffer, Martina
AU - Richter, Lars
AU - Schütte, Max L.
AU - Selbach, Florian
AU - Yaadav, Renukka
AU - Zähringer, Jonas
AU - Wei, Qingshan
AU - Ozcan, Aydogan
AU - Lalkens, Birka
AU - Acuna, Guillermo P.
AU - Tinnefeld, Philip
N1 - Funding information: The authors thank Vivien Behrendt and Benedikt Hauer (Fraunhofer-Institut für Phy-sikalische Messtechnik IPM, Freiburg, Germany) for cooperation on the assay development and Prof. Tim Liedl/Prof. Joachim Rädler (Ludwig-Maximilians-Universität, Department für Physik, Munich, Germany) for providing access to their facilities especially to the transmission electron microscope. The authors thank Tomas Gisicius for manufacturing the portable smartphone microscope. P.T. gratefully acknowledges financial support from the DFG (INST 86/1904-1 FUGG, excellence clusters NIM and e-conversion), BMBF (Grants POCEMON, 13N14336, and SIBOF, 03VP03891), and the European Union’s Horizon 2020 research and innovation program under grant agreement No. 737089 (Chipscope). G.P.A. gratefully acknowledges support by the Swiss National Science Foundation through the National Center of Competence in Research Bio-Inspired Materials and through grant number 200021_184687. V.G. and K.T. acknowledge the support by Humboldt Research Fellowships from the Alexander von Humboldt Foundation. A.O. acknowledges the support of NSF PATHS-UP and HHMI. BL acknowledges funding by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC-2123 QuantumFrontiers – 390837967 and “Niedersächsisches Vorab” through “Quantum-and Nano-Metrology (QUANOMET)” initiative within the project NL-1.
PY - 2021/2/11
Y1 - 2021/2/11
N2 - The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g., in point-of-care diagnostic settings, where costly equipment would be prohibitive. Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in NACHOS emit up to 461-fold (average of 89 ± 7-fold) brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia on the road.
AB - The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g., in point-of-care diagnostic settings, where costly equipment would be prohibitive. Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in NACHOS emit up to 461-fold (average of 89 ± 7-fold) brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia on the road.
UR - http://www.scopus.com/inward/record.url?scp=85100737294&partnerID=8YFLogxK
U2 - 10.1038/s41467-021-21238-9
DO - 10.1038/s41467-021-21238-9
M3 - Article
C2 - 33574261
AN - SCOPUS:85100737294
VL - 12
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 950
ER -