Details
| Original language | English |
|---|---|
| Pages (from-to) | 4193-4198 |
| Number of pages | 6 |
| Journal | Journal of crystal growth |
| Volume | 311 |
| Issue number | 17 |
| Publication status | Published - 9 Jul 2009 |
Abstract
In biotechnological and pharmaceutical engineering, the study of crystallization processes gains importance. An efficient analytical inline sensor could help to improve the knowledge about these processes in order to increase efficiency and yields. The in-situ microscope (ISM) is an optical sensor developed for the monitoring of bioprocesses. A new application for this sensor is the monitoring in downstream processes, e.g. the crystallization of proteins and other organic compounds. This contribution shows new aspects of using in-situ microscopy to monitor crystallization processes. Crystals of different chemical compounds were precipitated from supersaturated solutions and the crystal growth was monitored. Exemplified morphological properties and different forms of crystals could be distinguished on the basis of offline experiments. For inline monitoring of crystallization processes, a special 0.5 L stirred tank reactor was developed and equipped with the in-situ microscope. This reactor was utilized to carry out batch experiments for crystallizations of O-acetylsalicyclic acid (ASS) and hen egg white lysozyme (HEWL). During the whole crystallization process, the in-situ microscope system acquired images directly from the crystallization broth. For the data evaluation, an image analysis algorithm was developed and implemented in the microscope analysis software.
Keywords
- A1. Biocrystallization, A2. Crystal growth, A2. Crystal morphology, B1. ASS, B1. Lysozyme, B3. In-situ microscopy
ASJC Scopus subject areas
- Physics and Astronomy(all)
- Condensed Matter Physics
- Chemistry(all)
- Inorganic Chemistry
- Materials Science(all)
- Materials Chemistry
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In: Journal of crystal growth, Vol. 311, No. 17, 09.07.2009, p. 4193-4198.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Adaptation of in-situ microscopy for crystallization processes
AU - Bluma, A.
AU - Höpfner, T.
AU - Rudolph, G.
AU - Lindner, P.
AU - Beutel, S.
AU - Hitzmann, B.
AU - Scheper, T.
N1 - Funding information: The authors would like to thank the DFG for supporting the Project SCHE279/23-1.
PY - 2009/7/9
Y1 - 2009/7/9
N2 - In biotechnological and pharmaceutical engineering, the study of crystallization processes gains importance. An efficient analytical inline sensor could help to improve the knowledge about these processes in order to increase efficiency and yields. The in-situ microscope (ISM) is an optical sensor developed for the monitoring of bioprocesses. A new application for this sensor is the monitoring in downstream processes, e.g. the crystallization of proteins and other organic compounds. This contribution shows new aspects of using in-situ microscopy to monitor crystallization processes. Crystals of different chemical compounds were precipitated from supersaturated solutions and the crystal growth was monitored. Exemplified morphological properties and different forms of crystals could be distinguished on the basis of offline experiments. For inline monitoring of crystallization processes, a special 0.5 L stirred tank reactor was developed and equipped with the in-situ microscope. This reactor was utilized to carry out batch experiments for crystallizations of O-acetylsalicyclic acid (ASS) and hen egg white lysozyme (HEWL). During the whole crystallization process, the in-situ microscope system acquired images directly from the crystallization broth. For the data evaluation, an image analysis algorithm was developed and implemented in the microscope analysis software.
AB - In biotechnological and pharmaceutical engineering, the study of crystallization processes gains importance. An efficient analytical inline sensor could help to improve the knowledge about these processes in order to increase efficiency and yields. The in-situ microscope (ISM) is an optical sensor developed for the monitoring of bioprocesses. A new application for this sensor is the monitoring in downstream processes, e.g. the crystallization of proteins and other organic compounds. This contribution shows new aspects of using in-situ microscopy to monitor crystallization processes. Crystals of different chemical compounds were precipitated from supersaturated solutions and the crystal growth was monitored. Exemplified morphological properties and different forms of crystals could be distinguished on the basis of offline experiments. For inline monitoring of crystallization processes, a special 0.5 L stirred tank reactor was developed and equipped with the in-situ microscope. This reactor was utilized to carry out batch experiments for crystallizations of O-acetylsalicyclic acid (ASS) and hen egg white lysozyme (HEWL). During the whole crystallization process, the in-situ microscope system acquired images directly from the crystallization broth. For the data evaluation, an image analysis algorithm was developed and implemented in the microscope analysis software.
KW - A1. Biocrystallization
KW - A2. Crystal growth
KW - A2. Crystal morphology
KW - B1. ASS
KW - B1. Lysozyme
KW - B3. In-situ microscopy
UR - http://www.scopus.com/inward/record.url?scp=68549099695&partnerID=8YFLogxK
U2 - 10.1016/j.jcrysgro.2009.06.057
DO - 10.1016/j.jcrysgro.2009.06.057
M3 - Article
AN - SCOPUS:68549099695
VL - 311
SP - 4193
EP - 4198
JO - Journal of crystal growth
JF - Journal of crystal growth
SN - 0022-0248
IS - 17
ER -