Details
| Original language | English |
|---|---|
| Pages (from-to) | 132-138 |
| Number of pages | 7 |
| Journal | FEBS letters |
| Volume | 433 |
| Issue number | 1-2 |
| Publication status | Published - 14 Aug 1998 |
| Externally published | Yes |
Abstract
Acyl derivatives of type II PKS ACPs are required for in vitro studies of polyketide biosynthesis. The presence of an exposed cysteine residue prevented specific chemical acylation of the phosphopantetheine thiol of the actinorhodin PKS holo ACP. Acylation studies were further complicated by intramolecular disulphide formation between cysteine 17 and the phosphopantetheine. The presence of this intramolecular disulphide was confirmed by tryptic digestion of the ACP followed by ESMS analysis of the fragments. An act Cys17Ser ACP was engineered by site-directed mutagenesis. S-Acyl adducts of act C17S, oxytetracycline and griseusin holo ACPs were rapidly formed by reaction with hexanoyl, 5-ketohexanoyl and protected acetoacetyl imidazolides. Comparisons with type II FAS ACPs were made. Copyright (C) 1998 Federation of European Biochemical Societies.
Keywords
- Acyl carrier protein, Acylation, Fatty acid synthase, Polyketide synthase
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Biochemistry, Genetics and Molecular Biology(all)
- Structural Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: FEBS letters, Vol. 433, No. 1-2, 14.08.1998, p. 132-138.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Acylation of Streptomyces type II polyketide synthase acyl carrier proteins
AU - Crosby, John
AU - Byrom, Kate J.
AU - Hitchman, Timothy S.
AU - Cox, Russell J.
AU - Crump, Matthew P.
AU - Findlow, I. Stuart C.
AU - Bibb, Maureen J.
AU - Simpson, Thomas J.
N1 - Funding information: This work was supported by the Engineering and Physical Sciences Research Council (EPSRC), the Biotechnology and Biological Sciences Research Council (BBSRC) and the John Innes Foundation. Studentships from the EPSRC (I.S.C.F., T.S.H. and M.P.C.) and School of Chemistry (K.J.B.) are gratefully acknowledged. R.J.C. gratefully acknowledges the receipt of a Royal Society equipment grant.
PY - 1998/8/14
Y1 - 1998/8/14
N2 - Acyl derivatives of type II PKS ACPs are required for in vitro studies of polyketide biosynthesis. The presence of an exposed cysteine residue prevented specific chemical acylation of the phosphopantetheine thiol of the actinorhodin PKS holo ACP. Acylation studies were further complicated by intramolecular disulphide formation between cysteine 17 and the phosphopantetheine. The presence of this intramolecular disulphide was confirmed by tryptic digestion of the ACP followed by ESMS analysis of the fragments. An act Cys17Ser ACP was engineered by site-directed mutagenesis. S-Acyl adducts of act C17S, oxytetracycline and griseusin holo ACPs were rapidly formed by reaction with hexanoyl, 5-ketohexanoyl and protected acetoacetyl imidazolides. Comparisons with type II FAS ACPs were made. Copyright (C) 1998 Federation of European Biochemical Societies.
AB - Acyl derivatives of type II PKS ACPs are required for in vitro studies of polyketide biosynthesis. The presence of an exposed cysteine residue prevented specific chemical acylation of the phosphopantetheine thiol of the actinorhodin PKS holo ACP. Acylation studies were further complicated by intramolecular disulphide formation between cysteine 17 and the phosphopantetheine. The presence of this intramolecular disulphide was confirmed by tryptic digestion of the ACP followed by ESMS analysis of the fragments. An act Cys17Ser ACP was engineered by site-directed mutagenesis. S-Acyl adducts of act C17S, oxytetracycline and griseusin holo ACPs were rapidly formed by reaction with hexanoyl, 5-ketohexanoyl and protected acetoacetyl imidazolides. Comparisons with type II FAS ACPs were made. Copyright (C) 1998 Federation of European Biochemical Societies.
KW - Acyl carrier protein
KW - Acylation
KW - Fatty acid synthase
KW - Polyketide synthase
UR - http://www.scopus.com/inward/record.url?scp=0031872017&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(98)00840-0
DO - 10.1016/S0014-5793(98)00840-0
M3 - Article
C2 - 9738947
AN - SCOPUS:0031872017
VL - 433
SP - 132
EP - 138
JO - FEBS letters
JF - FEBS letters
SN - 0014-5793
IS - 1-2
ER -