Details
| Original language | English |
|---|---|
| Title of host publication | Plant Mitochondria |
| Subtitle of host publication | Methods and Protocols |
| Publisher | Springer New York |
| Pages | 131-138 |
| Number of pages | 8 |
| ISBN (electronic) | 9781493926398 |
| ISBN (print) | 9781493926381 |
| Publication status | Published - 24 Apr 2015 |
Abstract
In-gel activity assays are useful tools to identify and characterize enzymes within gels. Prerequisite are electrophoretic protein separations that are carried out under conditions compatible with enzyme activity. While blue native-polyacrylamide gel electrophoresis (BN-PAGE) is widely used for activity assays of the five enzyme complexes of the oxidative phosphorylation system, the blue background of this electrophoretic system is not compatible with activity assays for some other mitochondrial enzymes. As an alternative system, clear native (CN)-PAGE can be used for visualizing activities of mitochondrial enzymes. Here, we describe enzyme activity assays for mitochondrial enzymes in BN and CN gels.
Keywords
- Activity assays, BN-PAGE, CN-PAGE, In-gel assay
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- General Agricultural and Biological Sciences
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Plant Mitochondria: Methods and Protocols. Springer New York, 2015. p. 131-138.
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - Activity Measurements of Mitochondrial Enzymes in Native Gels
AU - Schertl, Peter
AU - Braun, Hans Peter
PY - 2015/4/24
Y1 - 2015/4/24
N2 - In-gel activity assays are useful tools to identify and characterize enzymes within gels. Prerequisite are electrophoretic protein separations that are carried out under conditions compatible with enzyme activity. While blue native-polyacrylamide gel electrophoresis (BN-PAGE) is widely used for activity assays of the five enzyme complexes of the oxidative phosphorylation system, the blue background of this electrophoretic system is not compatible with activity assays for some other mitochondrial enzymes. As an alternative system, clear native (CN)-PAGE can be used for visualizing activities of mitochondrial enzymes. Here, we describe enzyme activity assays for mitochondrial enzymes in BN and CN gels.
AB - In-gel activity assays are useful tools to identify and characterize enzymes within gels. Prerequisite are electrophoretic protein separations that are carried out under conditions compatible with enzyme activity. While blue native-polyacrylamide gel electrophoresis (BN-PAGE) is widely used for activity assays of the five enzyme complexes of the oxidative phosphorylation system, the blue background of this electrophoretic system is not compatible with activity assays for some other mitochondrial enzymes. As an alternative system, clear native (CN)-PAGE can be used for visualizing activities of mitochondrial enzymes. Here, we describe enzyme activity assays for mitochondrial enzymes in BN and CN gels.
KW - Activity assays
KW - BN-PAGE
KW - CN-PAGE
KW - In-gel assay
UR - http://www.scopus.com/inward/record.url?scp=84958639460&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-2639-8_9
DO - 10.1007/978-1-4939-2639-8_9
M3 - Contribution to book/anthology
AN - SCOPUS:84958639460
SN - 9781493926381
SP - 131
EP - 138
BT - Plant Mitochondria
PB - Springer New York
ER -