Details
| Original language | English |
|---|---|
| Article number | 998 |
| Journal | Cells |
| Volume | 10 |
| Issue number | 5 |
| Publication status | Published - 23 Apr 2021 |
Abstract
Aging and overweight increase the risk of developing type 2 diabetes mellitus. In this cross-sectional study, we aimed to investigate the potential mediating role of T-EMRA cells and inflammatory markers in the development of a decreased insulin sensitivity. A total of 134 healthy older volunteers were recruited (age 59.2 (SD 5.6) years). T cell subpopulations were analyzed by flow cytometry. Furthermore, body composition, HOMA-IR, plasma tryptophan (Trp) metabolites, as well as cytokines and adipokines were determined. Using subgroup and covariance analyses, the influence of BMI on the parameters was evaluated. Moreover, correlation, multiple regression, and mediation analyses were performed. In the subgroup of participants with obesity, an increased proportion of CD8+EMRA cells and elevated concentrations of plasma kynurenine (KYN) were found compared to the lower-weight subgroups. Linear regression analysis revealed that an elevated HOMA-IR could be predicted by a higher proportion of CD8+EMRA cells and KYN levels. A mediation analysis showed a robust indirect effect of the Waist-to-hip ratio on HOMA-IR mediated by CD8+EMRA cells. Thus, the deleterious effects of abdominal obesity on glucose metabolism might be mediated by CD8+EMRA cells in the elderly. Longitudinal studies should validate this assumption and analyze the suitability of CD8+EMRA cells as early predictors of incipient prediabetes.
Keywords
- T-EMRA cells, elderly, insulin resistance, kynurenine pathway, obesity, Obesity, T‐EMRA cells, Insulin resistance, Elderly, Kynurenine pathway
ASJC Scopus subject areas
- Medicine(all)
- General Medicine
Sustainable Development Goals
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In: Cells, Vol. 10, No. 5, 998, 23.04.2021.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Abdominal Obesity-Related Disturbance of Insulin Sensitivity Is Associated with CD8+ EMRA Cells in the Elderly
AU - Boßlau, Tim K.
AU - Wasserfurth, Paulina
AU - Krüger, Britta
AU - Reichel, Thomas
AU - Palmowski, Jana
AU - Nebl, Josefine
AU - Weyh, Christopher
AU - Schenk, Alexander
AU - Joisten, Niklas
AU - Stahl, Frank
AU - Thoms, Stefanie
AU - Gebhardt, Kristina
AU - Hahn, Andreas
AU - Krüger, Karsten
PY - 2021/4/23
Y1 - 2021/4/23
N2 - Aging and overweight increase the risk of developing type 2 diabetes mellitus. In this cross-sectional study, we aimed to investigate the potential mediating role of T-EMRA cells and inflammatory markers in the development of a decreased insulin sensitivity. A total of 134 healthy older volunteers were recruited (age 59.2 (SD 5.6) years). T cell subpopulations were analyzed by flow cytometry. Furthermore, body composition, HOMA-IR, plasma tryptophan (Trp) metabolites, as well as cytokines and adipokines were determined. Using subgroup and covariance analyses, the influence of BMI on the parameters was evaluated. Moreover, correlation, multiple regression, and mediation analyses were performed. In the subgroup of participants with obesity, an increased proportion of CD8+EMRA cells and elevated concentrations of plasma kynurenine (KYN) were found compared to the lower-weight subgroups. Linear regression analysis revealed that an elevated HOMA-IR could be predicted by a higher proportion of CD8+EMRA cells and KYN levels. A mediation analysis showed a robust indirect effect of the Waist-to-hip ratio on HOMA-IR mediated by CD8+EMRA cells. Thus, the deleterious effects of abdominal obesity on glucose metabolism might be mediated by CD8+EMRA cells in the elderly. Longitudinal studies should validate this assumption and analyze the suitability of CD8+EMRA cells as early predictors of incipient prediabetes.
AB - Aging and overweight increase the risk of developing type 2 diabetes mellitus. In this cross-sectional study, we aimed to investigate the potential mediating role of T-EMRA cells and inflammatory markers in the development of a decreased insulin sensitivity. A total of 134 healthy older volunteers were recruited (age 59.2 (SD 5.6) years). T cell subpopulations were analyzed by flow cytometry. Furthermore, body composition, HOMA-IR, plasma tryptophan (Trp) metabolites, as well as cytokines and adipokines were determined. Using subgroup and covariance analyses, the influence of BMI on the parameters was evaluated. Moreover, correlation, multiple regression, and mediation analyses were performed. In the subgroup of participants with obesity, an increased proportion of CD8+EMRA cells and elevated concentrations of plasma kynurenine (KYN) were found compared to the lower-weight subgroups. Linear regression analysis revealed that an elevated HOMA-IR could be predicted by a higher proportion of CD8+EMRA cells and KYN levels. A mediation analysis showed a robust indirect effect of the Waist-to-hip ratio on HOMA-IR mediated by CD8+EMRA cells. Thus, the deleterious effects of abdominal obesity on glucose metabolism might be mediated by CD8+EMRA cells in the elderly. Longitudinal studies should validate this assumption and analyze the suitability of CD8+EMRA cells as early predictors of incipient prediabetes.
KW - T-EMRA cells
KW - elderly
KW - insulin resistance
KW - kynurenine pathway
KW - obesity
KW - Obesity
KW - T‐EMRA cells
KW - Insulin resistance
KW - Elderly
KW - Kynurenine pathway
UR - http://www.scopus.com/inward/record.url?scp=85105194436&partnerID=8YFLogxK
U2 - 10.3390/cells10050998
DO - 10.3390/cells10050998
M3 - Article
VL - 10
JO - Cells
JF - Cells
SN - 2073-4409
IS - 5
M1 - 998
ER -