Details
| Original language | English |
|---|---|
| Pages (from-to) | 67-77 |
| Number of pages | 11 |
| Journal | Plant science |
| Volume | 152 |
| Issue number | 1 |
| Publication status | Published - 7 Mar 2000 |
| Externally published | Yes |
Abstract
Five transcripts encoding different members of a nodulin family with conserved cysteine clusters (Cys-X4-Asp-Cys and Cys-X4-Cys) were identified in broad bean root nodules. They displayed homologies to the early nodulins PsENOD3 and PsENOD14 and the late nodulin PsNOD6 from pea. In addition to the occurrence of putative secretory signal peptides, the spatial distribution of the cysteine residues was comparable in both the broad bean and the pea nodulins. Based on tissue print hybridizations, we found that the corresponding broad bean genes VfNOD-CCP1, VfNOD-CCP3 and VfNOD-CCP5 were expressed in the interzone II-III and the nitrogen fixing zone III of mature nodules whereas the gene VfNOD-CCP4 was first induced in the prefixing zone II. A strong expression of the VfNOD-CCP2 gene only could be detected the interzone II-III region. Sequence analysis of a genomic VfNOD-CCP1 clone isolated revealed the presence of one intron seperating a first exon encoding the signal peptide from a second exon encoding the cysteine cluster domain of this nodulin. Apart from the multiple presence of the common nodulin motifs AAAGAT and CTCTT on both DNA strands of the putative VfNOD-CCP1 promoter region a sequence element resembling the organ specific element of the soybean lbc3 gene promoter was identified. (C) 2000 Elsevier Science Ireland Ltd.
Keywords
- Metal binding, Symbiotic nitrogen fixation, Tissue print hybridization, Vicia faba L.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant science, Vol. 152, No. 1, 07.03.2000, p. 67-77.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A small gene family of broad bean codes for late nodulins containing conserved cysteine clusters
AU - Frühling, Martin
AU - Albus, Ulrike
AU - Hohnjec, Natalija
AU - Geise, Gerhard
AU - Pühler, Alfred
AU - Perlick, Andreas M.
PY - 2000/3/7
Y1 - 2000/3/7
N2 - Five transcripts encoding different members of a nodulin family with conserved cysteine clusters (Cys-X4-Asp-Cys and Cys-X4-Cys) were identified in broad bean root nodules. They displayed homologies to the early nodulins PsENOD3 and PsENOD14 and the late nodulin PsNOD6 from pea. In addition to the occurrence of putative secretory signal peptides, the spatial distribution of the cysteine residues was comparable in both the broad bean and the pea nodulins. Based on tissue print hybridizations, we found that the corresponding broad bean genes VfNOD-CCP1, VfNOD-CCP3 and VfNOD-CCP5 were expressed in the interzone II-III and the nitrogen fixing zone III of mature nodules whereas the gene VfNOD-CCP4 was first induced in the prefixing zone II. A strong expression of the VfNOD-CCP2 gene only could be detected the interzone II-III region. Sequence analysis of a genomic VfNOD-CCP1 clone isolated revealed the presence of one intron seperating a first exon encoding the signal peptide from a second exon encoding the cysteine cluster domain of this nodulin. Apart from the multiple presence of the common nodulin motifs AAAGAT and CTCTT on both DNA strands of the putative VfNOD-CCP1 promoter region a sequence element resembling the organ specific element of the soybean lbc3 gene promoter was identified. (C) 2000 Elsevier Science Ireland Ltd.
AB - Five transcripts encoding different members of a nodulin family with conserved cysteine clusters (Cys-X4-Asp-Cys and Cys-X4-Cys) were identified in broad bean root nodules. They displayed homologies to the early nodulins PsENOD3 and PsENOD14 and the late nodulin PsNOD6 from pea. In addition to the occurrence of putative secretory signal peptides, the spatial distribution of the cysteine residues was comparable in both the broad bean and the pea nodulins. Based on tissue print hybridizations, we found that the corresponding broad bean genes VfNOD-CCP1, VfNOD-CCP3 and VfNOD-CCP5 were expressed in the interzone II-III and the nitrogen fixing zone III of mature nodules whereas the gene VfNOD-CCP4 was first induced in the prefixing zone II. A strong expression of the VfNOD-CCP2 gene only could be detected the interzone II-III region. Sequence analysis of a genomic VfNOD-CCP1 clone isolated revealed the presence of one intron seperating a first exon encoding the signal peptide from a second exon encoding the cysteine cluster domain of this nodulin. Apart from the multiple presence of the common nodulin motifs AAAGAT and CTCTT on both DNA strands of the putative VfNOD-CCP1 promoter region a sequence element resembling the organ specific element of the soybean lbc3 gene promoter was identified. (C) 2000 Elsevier Science Ireland Ltd.
KW - Metal binding
KW - Symbiotic nitrogen fixation
KW - Tissue print hybridization
KW - Vicia faba L.
UR - http://www.scopus.com/inward/record.url?scp=0034615515&partnerID=8YFLogxK
U2 - 10.1016/S0168-9452(99)00219-8
DO - 10.1016/S0168-9452(99)00219-8
M3 - Article
AN - SCOPUS:0034615515
VL - 152
SP - 67
EP - 77
JO - Plant science
JF - Plant science
SN - 0168-9452
IS - 1
ER -