Details
| Original language | English |
|---|---|
| Article number | 037001 |
| Journal | Methods and Applications in Fluorescence |
| Volume | 12 |
| Issue number | 3 |
| Publication status | Published - 17 Jun 2024 |
Abstract
Keywords
- device, front-face, fluorescence, scattering, solid sample
ASJC Scopus subject areas
- Physics and Astronomy(all)
- Instrumentation
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Materials Science(all)
- Chemistry(all)
- Spectroscopy
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In: Methods and Applications in Fluorescence, Vol. 12, No. 3, 037001, 17.06.2024.
Research output: Contribution to journal › Article › Transfer › peer review
}
TY - JOUR
T1 - A simple light path modifying device to reduce scattering in front-face fluorescence spectra
AU - Peters, Frank B.
AU - Rapp, Andreas Otto
N1 - Publisher Copyright: © 2024 The Author(s). Published by IOP Publishing Ltd.
PY - 2024/6/17
Y1 - 2024/6/17
N2 - This technical note presents a device to diminish scattering signal in front-face fluorescence spectra while obtaining fluorescence signal. The beam path in a commercial fluorescence spectrometer was modified by two deflecting mirrors, leading reflections away from the sensor. This light path modifying (LPM) device was tested with two fluid and three solid substances, where the scattering-to-fluorescence ratio improved by a factor of 1.7 to 7.6. The spectra obtained with the LPM were much clearer, and distortion of the fluorescence peaks was avoided. Scans of quinine sulphate complied well with reference spectra.
AB - This technical note presents a device to diminish scattering signal in front-face fluorescence spectra while obtaining fluorescence signal. The beam path in a commercial fluorescence spectrometer was modified by two deflecting mirrors, leading reflections away from the sensor. This light path modifying (LPM) device was tested with two fluid and three solid substances, where the scattering-to-fluorescence ratio improved by a factor of 1.7 to 7.6. The spectra obtained with the LPM were much clearer, and distortion of the fluorescence peaks was avoided. Scans of quinine sulphate complied well with reference spectra.
KW - device
KW - front-face
KW - fluorescence
KW - scattering
KW - solid sample
UR - http://www.scopus.com/inward/record.url?scp=85196326115&partnerID=8YFLogxK
U2 - 10.1088/2050-6120/ad5415
DO - 10.1088/2050-6120/ad5415
M3 - Article
VL - 12
JO - Methods and Applications in Fluorescence
JF - Methods and Applications in Fluorescence
SN - 2050-6120
IS - 3
M1 - 037001
ER -