Details
| Original language | English |
|---|---|
| Pages (from-to) | 671-678 |
| Number of pages | 8 |
| Journal | Biotechnology progress |
| Volume | 26 |
| Issue number | 3 |
| Publication status | Published - 29 Jan 2010 |
Abstract
The development of bone tissue engineering depends on the availability of suitable biomaterials, a well-defined and controlled bioreactor system, and on the use of adequate cells. The biomaterial must fulfill chemical, biological, and mechanical requirements. Besides biocompatibility, the structural and flow characteristics of the biomaterial are of utmost importance for a successful dynamic cultivation of osteoblasts, since fluid percolation within the microstructure must be assured to supply to cells nutrients and waste removal. Therefore, the biomaterial must consist of a three-dimensional structure, exhibit high porosity and present an interconnected porous network. Sponceram®, a ZrO2 based porous ceramic, is characterized in the presented work with regard to its microstructural design. Intrinsic permeability is obtained through a standard Darcy's experiment, while Young's modulus is derived from a two plates stress-strain test in the linear range. Furthermore, the material is applied for the dynamic cultivation of primary osteoblasts in a newly developed rotating bed bioreactor.
Keywords
- Biomechanics, Primary osteoblasts, Rotating bed system, Sponceram®, Young's modulus
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
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In: Biotechnology progress, Vol. 26, No. 3, 29.01.2010, p. 671-678.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A Rotating Bed System Bioreactor Enables Cultivation of Primary Osteoblasts onWell-Characterized Sponceram® Regarding Structural and Flow Properties
AU - Suck, Kirstin
AU - Roeker, Stefanie
AU - Diederichs, Solvig
AU - Anton, Fabienne
AU - Sanz-Herrera, Jose A.
AU - Ochoa, Ignacio
AU - Doblare, Manuel
AU - Scheper, Thomas
AU - van Griensven, Martijn
AU - Kasper, Cornelia
PY - 2010/1/29
Y1 - 2010/1/29
N2 - The development of bone tissue engineering depends on the availability of suitable biomaterials, a well-defined and controlled bioreactor system, and on the use of adequate cells. The biomaterial must fulfill chemical, biological, and mechanical requirements. Besides biocompatibility, the structural and flow characteristics of the biomaterial are of utmost importance for a successful dynamic cultivation of osteoblasts, since fluid percolation within the microstructure must be assured to supply to cells nutrients and waste removal. Therefore, the biomaterial must consist of a three-dimensional structure, exhibit high porosity and present an interconnected porous network. Sponceram®, a ZrO2 based porous ceramic, is characterized in the presented work with regard to its microstructural design. Intrinsic permeability is obtained through a standard Darcy's experiment, while Young's modulus is derived from a two plates stress-strain test in the linear range. Furthermore, the material is applied for the dynamic cultivation of primary osteoblasts in a newly developed rotating bed bioreactor.
AB - The development of bone tissue engineering depends on the availability of suitable biomaterials, a well-defined and controlled bioreactor system, and on the use of adequate cells. The biomaterial must fulfill chemical, biological, and mechanical requirements. Besides biocompatibility, the structural and flow characteristics of the biomaterial are of utmost importance for a successful dynamic cultivation of osteoblasts, since fluid percolation within the microstructure must be assured to supply to cells nutrients and waste removal. Therefore, the biomaterial must consist of a three-dimensional structure, exhibit high porosity and present an interconnected porous network. Sponceram®, a ZrO2 based porous ceramic, is characterized in the presented work with regard to its microstructural design. Intrinsic permeability is obtained through a standard Darcy's experiment, while Young's modulus is derived from a two plates stress-strain test in the linear range. Furthermore, the material is applied for the dynamic cultivation of primary osteoblasts in a newly developed rotating bed bioreactor.
KW - Biomechanics
KW - Primary osteoblasts
KW - Rotating bed system
KW - Sponceram®
KW - Young's modulus
UR - http://www.scopus.com/inward/record.url?scp=77954702569&partnerID=8YFLogxK
U2 - 10.1002/btpr.386
DO - 10.1002/btpr.386
M3 - Article
C2 - 20196152
AN - SCOPUS:77954702569
VL - 26
SP - 671
EP - 678
JO - Biotechnology progress
JF - Biotechnology progress
SN - 8756-7938
IS - 3
ER -