Details
| Original language | English |
|---|---|
| Pages (from-to) | 866-877 |
| Number of pages | 12 |
| Journal | Environmental microbiology |
| Volume | 24 |
| Issue number | 2 |
| Early online date | 18 Oct 2021 |
| Publication status | Published - 25 Feb 2022 |
| Externally published | Yes |
Abstract
Iron is an essential nutrient for bacterial growth but poorly bioavailable. Bacteria scavenge ferric iron by synthesizing and secreting siderophores, small compounds with a high affinity for iron. Pyochelin (PCH) is one of the two siderophores produced by the opportunistic pathogen Pseudomonas aeruginosa. After capturing a ferric iron molecule, PCH-Fe is imported back into bacteria first by the outer membrane transporter FptA and then by the inner membrane permease FptX. Here, using molecular biology, 55Fe uptake assays, and LC–MS/MS quantification, we first find a role for PchHI as the heterodimeric ABC transporter involved in the siderophore-free iron uptake into the bacterial cytoplasm. We also provide the first evidence that PCH is able to reach the bacterial periplasm and cytoplasm when both FptA and FptX are expressed. Finally, we detected an interaction between PchH and FptX, linking the ABC transporter PchHI with the inner permease FptX in the PCH-Fe uptake pathway. These results pave the way for a better understanding of the PCH siderophore pathway, giving future directions to tackle P. aeruginosa infections.
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Agricultural and Biological Sciences(all)
- Ecology, Evolution, Behavior and Systematics
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In: Environmental microbiology, Vol. 24, No. 2, 25.02.2022, p. 866-877.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A role for PchHI as the ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa
AU - Roche, Béatrice
AU - Garcia-Rivera, Mariel A.
AU - Normant, Vincent
AU - Kuhn, Lauriane
AU - Hammann, Philippe
AU - Brönstrup, Mark
AU - Mislin, Gaëtan L.A.
AU - Schalk, Isabelle J.
N1 - Funding Information: Authors acknowledge the Centre National de la Recherche Scientifique (CNRS) for general financial support. BR would like to thank Roche Pharmaceutical Research and Early Development Basel for their financial support via the Roche Postdoctoral Fellowship (RPF) Program. We thank Aurélia Battesti (LCB, Marseille) for kindly providing the empty two‐hybrid vectors. The mass spectrometry instrumentation was funded by the University of Strasbourg, IdEx 'Equipement mi‐lourd' 2015. We also received support by a grant from the Joint Programming Initiative on Antimicrobial Resistance (JPI AMR, grant number: 01K11825).
PY - 2022/2/25
Y1 - 2022/2/25
N2 - Iron is an essential nutrient for bacterial growth but poorly bioavailable. Bacteria scavenge ferric iron by synthesizing and secreting siderophores, small compounds with a high affinity for iron. Pyochelin (PCH) is one of the two siderophores produced by the opportunistic pathogen Pseudomonas aeruginosa. After capturing a ferric iron molecule, PCH-Fe is imported back into bacteria first by the outer membrane transporter FptA and then by the inner membrane permease FptX. Here, using molecular biology, 55Fe uptake assays, and LC–MS/MS quantification, we first find a role for PchHI as the heterodimeric ABC transporter involved in the siderophore-free iron uptake into the bacterial cytoplasm. We also provide the first evidence that PCH is able to reach the bacterial periplasm and cytoplasm when both FptA and FptX are expressed. Finally, we detected an interaction between PchH and FptX, linking the ABC transporter PchHI with the inner permease FptX in the PCH-Fe uptake pathway. These results pave the way for a better understanding of the PCH siderophore pathway, giving future directions to tackle P. aeruginosa infections.
AB - Iron is an essential nutrient for bacterial growth but poorly bioavailable. Bacteria scavenge ferric iron by synthesizing and secreting siderophores, small compounds with a high affinity for iron. Pyochelin (PCH) is one of the two siderophores produced by the opportunistic pathogen Pseudomonas aeruginosa. After capturing a ferric iron molecule, PCH-Fe is imported back into bacteria first by the outer membrane transporter FptA and then by the inner membrane permease FptX. Here, using molecular biology, 55Fe uptake assays, and LC–MS/MS quantification, we first find a role for PchHI as the heterodimeric ABC transporter involved in the siderophore-free iron uptake into the bacterial cytoplasm. We also provide the first evidence that PCH is able to reach the bacterial periplasm and cytoplasm when both FptA and FptX are expressed. Finally, we detected an interaction between PchH and FptX, linking the ABC transporter PchHI with the inner permease FptX in the PCH-Fe uptake pathway. These results pave the way for a better understanding of the PCH siderophore pathway, giving future directions to tackle P. aeruginosa infections.
UR - http://www.scopus.com/inward/record.url?scp=85117180587&partnerID=8YFLogxK
U2 - 10.1111/1462-2920.15811
DO - 10.1111/1462-2920.15811
M3 - Article
AN - SCOPUS:85117180587
VL - 24
SP - 866
EP - 877
JO - Environmental microbiology
JF - Environmental microbiology
SN - 1462-2912
IS - 2
ER -