Details
| Original language | English |
|---|---|
| Pages (from-to) | 247-256 |
| Number of pages | 10 |
| Journal | Bioprocess and Biosystems Engineering |
| Volume | 33 |
| Issue number | 2 |
| Publication status | Published - 25 Apr 2009 |
Abstract
To observe and control cultivation processes, optical sensors are used increasingly. Important variables for controlling such processes are cell count, cell size distribution and the morphology of cells. Among turbidity measurement methods, imaging procedures are applied for determining these process values. A disadvantage of most previously developed imaging procedures is that they are only available offline, which requires sampling. On the other hand, available imaging inline probes can only deliver a limited number of process values so far. This contribution gives an overview of optical procedures for the inline determination of cell count, cell size distribution and other variables. In particular, by in situ microscopy, an imaging procedure will be described, which allows the determination of direct and non-direct cell variables in real time without sampling.
Keywords
- Cultivation, Focused beam reflectance measurement (FBRM), Growth, In situ-microscopy, Particle size
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Bioprocess and Biosystems Engineering, Vol. 33, No. 2, 25.04.2009, p. 247-256.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - A review of non-invasive optical-based image analysis systems for continuous bioprocess monitoring
AU - Höpfner, Tim
AU - Bluma, Arne
AU - Rudolph, Guido
AU - Lindner, Patrick
AU - Scheper, Thomas
PY - 2009/4/25
Y1 - 2009/4/25
N2 - To observe and control cultivation processes, optical sensors are used increasingly. Important variables for controlling such processes are cell count, cell size distribution and the morphology of cells. Among turbidity measurement methods, imaging procedures are applied for determining these process values. A disadvantage of most previously developed imaging procedures is that they are only available offline, which requires sampling. On the other hand, available imaging inline probes can only deliver a limited number of process values so far. This contribution gives an overview of optical procedures for the inline determination of cell count, cell size distribution and other variables. In particular, by in situ microscopy, an imaging procedure will be described, which allows the determination of direct and non-direct cell variables in real time without sampling.
AB - To observe and control cultivation processes, optical sensors are used increasingly. Important variables for controlling such processes are cell count, cell size distribution and the morphology of cells. Among turbidity measurement methods, imaging procedures are applied for determining these process values. A disadvantage of most previously developed imaging procedures is that they are only available offline, which requires sampling. On the other hand, available imaging inline probes can only deliver a limited number of process values so far. This contribution gives an overview of optical procedures for the inline determination of cell count, cell size distribution and other variables. In particular, by in situ microscopy, an imaging procedure will be described, which allows the determination of direct and non-direct cell variables in real time without sampling.
KW - Cultivation
KW - Focused beam reflectance measurement (FBRM)
KW - Growth
KW - In situ-microscopy
KW - Particle size
UR - http://www.scopus.com/inward/record.url?scp=77649234358&partnerID=8YFLogxK
U2 - 10.1007/s00449-009-0319-8
DO - 10.1007/s00449-009-0319-8
M3 - Review article
C2 - 19396466
AN - SCOPUS:77649234358
VL - 33
SP - 247
EP - 256
JO - Bioprocess and Biosystems Engineering
JF - Bioprocess and Biosystems Engineering
SN - 1615-7591
IS - 2
ER -