Details
| Original language | English |
|---|---|
| Pages (from-to) | 4532-4537 |
| Number of pages | 6 |
| Journal | Biosensors and Bioelectronics |
| Volume | 26 |
| Issue number | 11 |
| Publication status | Published - 19 May 2011 |
Abstract
A multi-analyte sensing device is described, for simultaneous at-line monitoring of glucose, ethanol, pO 2-value and cell density. It consists of a dual biosensor, a modified microscope and a fiber optical pO 2-sensor that are integrated into a flow analysis (FA) system.The biosensor is based on a conventional thin layer flow-through cell equipped with a gold (Au) dual electrode (serial configuration). The biosensors with no cross-talking were produced by modifying the electrochemical transducers. Each Au surface was initially modified by self-assembled monolayer (SAM) of cysteamine. Alcohol oxidase (AOx) and pyranose oxidase (PyOx) were immobilized each onto a gold surface by means of PAMAM (polyamidoamine) dendrimer via glutaraldehyde cross-linking. The responses for glucose and ethanol were linear up to 0.5. mM. The operational stability of the biosensors was very promising, after 11. h continuous operation, only 6.0% of the initial activity was lost. The potential of the described biosensor was demonstrated by parallel determination of ethanol and glucose in yeast fermentation process.Simultaneously the cell density of the culture was monitored with an in situ microscope (ISM), which was integrated into the FA system. Both the used in situ microscope and the image processing algorithm used for the analysis of the acquired image data are described. Furthermore the pO 2-value was monitored using a fiber optical sensor, which was embedded in a flow cell. The multi-sensor device allows the at-line monitoring of several process values without the need for further sampling or time consuming offline measurements.
Keywords
- Biosensor, In situ microscopy, Multi-analyte sensing, Yeast fermentation
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Engineering(all)
- Biomedical Engineering
- Chemistry(all)
- Electrochemistry
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In: Biosensors and Bioelectronics, Vol. 26, No. 11, 19.05.2011, p. 4532-4537.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A new set up for multi-analyte sensing: At-line bio-process monitoring
AU - Akin, Mehriban
AU - Prediger, Andreas
AU - Yuksel, Merve
AU - Höpfner, Tim
AU - Demirkol, Dilek Odaci
AU - Beutel, Sascha
AU - Timur, Suna
AU - Scheper, Thomas
N1 - Funding information: This work is supported by Scientific and Technological Research Council of Turkey (TUBITAK) under the Research Project number 108T293 and Federal Ministry of Education and Research (BMBF) under the project number TUR08/004. Ege University, Research and Application Center of Science and Technology (EBILTEM) , is acknowledged for the research grants (Project number; 2009 BIL 008).
PY - 2011/5/19
Y1 - 2011/5/19
N2 - A multi-analyte sensing device is described, for simultaneous at-line monitoring of glucose, ethanol, pO 2-value and cell density. It consists of a dual biosensor, a modified microscope and a fiber optical pO 2-sensor that are integrated into a flow analysis (FA) system.The biosensor is based on a conventional thin layer flow-through cell equipped with a gold (Au) dual electrode (serial configuration). The biosensors with no cross-talking were produced by modifying the electrochemical transducers. Each Au surface was initially modified by self-assembled monolayer (SAM) of cysteamine. Alcohol oxidase (AOx) and pyranose oxidase (PyOx) were immobilized each onto a gold surface by means of PAMAM (polyamidoamine) dendrimer via glutaraldehyde cross-linking. The responses for glucose and ethanol were linear up to 0.5. mM. The operational stability of the biosensors was very promising, after 11. h continuous operation, only 6.0% of the initial activity was lost. The potential of the described biosensor was demonstrated by parallel determination of ethanol and glucose in yeast fermentation process.Simultaneously the cell density of the culture was monitored with an in situ microscope (ISM), which was integrated into the FA system. Both the used in situ microscope and the image processing algorithm used for the analysis of the acquired image data are described. Furthermore the pO 2-value was monitored using a fiber optical sensor, which was embedded in a flow cell. The multi-sensor device allows the at-line monitoring of several process values without the need for further sampling or time consuming offline measurements.
AB - A multi-analyte sensing device is described, for simultaneous at-line monitoring of glucose, ethanol, pO 2-value and cell density. It consists of a dual biosensor, a modified microscope and a fiber optical pO 2-sensor that are integrated into a flow analysis (FA) system.The biosensor is based on a conventional thin layer flow-through cell equipped with a gold (Au) dual electrode (serial configuration). The biosensors with no cross-talking were produced by modifying the electrochemical transducers. Each Au surface was initially modified by self-assembled monolayer (SAM) of cysteamine. Alcohol oxidase (AOx) and pyranose oxidase (PyOx) were immobilized each onto a gold surface by means of PAMAM (polyamidoamine) dendrimer via glutaraldehyde cross-linking. The responses for glucose and ethanol were linear up to 0.5. mM. The operational stability of the biosensors was very promising, after 11. h continuous operation, only 6.0% of the initial activity was lost. The potential of the described biosensor was demonstrated by parallel determination of ethanol and glucose in yeast fermentation process.Simultaneously the cell density of the culture was monitored with an in situ microscope (ISM), which was integrated into the FA system. Both the used in situ microscope and the image processing algorithm used for the analysis of the acquired image data are described. Furthermore the pO 2-value was monitored using a fiber optical sensor, which was embedded in a flow cell. The multi-sensor device allows the at-line monitoring of several process values without the need for further sampling or time consuming offline measurements.
KW - Biosensor
KW - In situ microscopy
KW - Multi-analyte sensing
KW - Yeast fermentation
UR - http://www.scopus.com/inward/record.url?scp=79959230564&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2011.05.018
DO - 10.1016/j.bios.2011.05.018
M3 - Article
C2 - 21664121
AN - SCOPUS:79959230564
VL - 26
SP - 4532
EP - 4537
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
IS - 11
ER -