Details
Original language | English |
---|---|
Pages (from-to) | 201-217 |
Number of pages | 17 |
Journal | Journal of biotechnology |
Volume | 38 |
Issue number | 3 |
Publication status | Published - 31 Jan 1995 |
Abstract
A model that was employed in simulations of the development of single fungal pellets was extended in order to describe Penicillium chrysogenum fed-batch cultivations. Spore germination, pre- and main culture of up to 100 pellets were simulated, each of which represents a subpopulation of the entire biomass. Experimentally observed pellet size distributions at the beginning of the main cultivation are explained by different times of spore germination. In the model, penicillin production was coupled to the morphology (active cells not containing tips) and described by a simple first-order rate equation. The experimental data of biomass, glucose, penicillin concentration, and pellet size distribution could only be described if pellet fragmentation was included in the model, which also explains the decreasing pellet size during cultivation in a stirred tank. It was assumed that the growth activity of pellet fragments is impaired because of cell damage, repair activities and other influences. The model was submitted to a sensitivity analysis and compared to a previously presented segregated model.
Keywords
- Fed-batch cultivation, Model, Morphology, Pellet, Shear force
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of biotechnology, Vol. 38, No. 3, 31.01.1995, p. 201-217.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A morphology-based model for fed-batch cultivations of Penicillium chrysogenum growing in pellet form
AU - Meyerhoff, Juliane
AU - Bellgardt, Karl heinz
PY - 1995/1/31
Y1 - 1995/1/31
N2 - A model that was employed in simulations of the development of single fungal pellets was extended in order to describe Penicillium chrysogenum fed-batch cultivations. Spore germination, pre- and main culture of up to 100 pellets were simulated, each of which represents a subpopulation of the entire biomass. Experimentally observed pellet size distributions at the beginning of the main cultivation are explained by different times of spore germination. In the model, penicillin production was coupled to the morphology (active cells not containing tips) and described by a simple first-order rate equation. The experimental data of biomass, glucose, penicillin concentration, and pellet size distribution could only be described if pellet fragmentation was included in the model, which also explains the decreasing pellet size during cultivation in a stirred tank. It was assumed that the growth activity of pellet fragments is impaired because of cell damage, repair activities and other influences. The model was submitted to a sensitivity analysis and compared to a previously presented segregated model.
AB - A model that was employed in simulations of the development of single fungal pellets was extended in order to describe Penicillium chrysogenum fed-batch cultivations. Spore germination, pre- and main culture of up to 100 pellets were simulated, each of which represents a subpopulation of the entire biomass. Experimentally observed pellet size distributions at the beginning of the main cultivation are explained by different times of spore germination. In the model, penicillin production was coupled to the morphology (active cells not containing tips) and described by a simple first-order rate equation. The experimental data of biomass, glucose, penicillin concentration, and pellet size distribution could only be described if pellet fragmentation was included in the model, which also explains the decreasing pellet size during cultivation in a stirred tank. It was assumed that the growth activity of pellet fragments is impaired because of cell damage, repair activities and other influences. The model was submitted to a sensitivity analysis and compared to a previously presented segregated model.
KW - Fed-batch cultivation
KW - Model
KW - Morphology
KW - Pellet
KW - Shear force
UR - http://www.scopus.com/inward/record.url?scp=0028888256&partnerID=8YFLogxK
U2 - 10.1016/0168-1656(94)00121-R
DO - 10.1016/0168-1656(94)00121-R
M3 - Article
AN - SCOPUS:0028888256
VL - 38
SP - 201
EP - 217
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 3
ER -